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Procalcitonin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:PCT
  • Species:Human
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Cat. No. Size Price


RD191006200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine

Sample Requirements

serum 50 ul/well
urine 75 ul/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

50–3200 pg/ml

Limit of Detection

15 pg/ml

Intra-assay (Within-Run)

n = 8; CV = 2.7 %

Inter-assay (Run-to-Run)

n = 6; CV = 4.8 %

Spiking Recovery

101,80%

Dilution Linearity

98,60%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • dog Yes
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • chicken Not tested
  • human Yes
  • sheep Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 4 hours
  • The kit measures procalcitonin in serum and urine
  • Assay format is 96 wells
  • Standard is recombinant protein based
  • Quality Controls are human serum based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Immune Response, Infection and Inflammation, Sepsis

Summary

Procalcitonin (PCT) the precursor of the hormone calcitonin is a 116 amino acid protein with a
molecular mass of 13 kDa. It undergoes successive cleavages in the neuroendocrine cells of
the thyroid to form three distinct molecules: calcitonin (32 amino acids); katacalcin (21 amino
acids) and N-terminal fragment called aminoprocalcitonin (57 amino acids). Procalcitonin
belongs to a group of related proteins including calcitonin gene-related peptides I and II,
amylin, adrenomodulin and calcitonin (CAPA peptide family). Synthesis of procalcitonin is
regulated gene CALC-1. Under normal metabolic conditions procalcitonin is present in the
C-cells of the thyroid gland. The level of procalcitonin in the blood of healthy individuals is low.
The risk of local bacterial infection occurs when the value of procalcitonin exceeds 0.25 ng/ml.
The risk of systemic bacterial infection occurs when the value of procalcitonin exceeds 0.5
ng/ml. Bacterial lipopolysacharide (LPS) has been shown to be a potent inducer of
procalcitonin release into systemic circulation. This release is not associated with an increase
in calcitonin. Procalcitonin levels increase from 3 to 4 hours, peak at about 6 hours and then
plateau for up to 24 hours. In contrast, C-reactive protein (CRP) levels rise between 12 and 18
hours after bacterial challenge. In blood serum, procalcitonin has a half-life of between 25 and
30 hours. A study showed that hepatocytes produce large amounts of procalcitonin following
stimulation with TNF-α and IL-6. In acute pancreatitis, procalcitonin closely correlates with the
development of pancreatic infections. Since procalcitonin has been reported to be increased in
different non-septic conditions such as major trauma, acute respiratory distress syndrome,
rejection after transplantation, cardiogenic shock, severe burns and heat-stroke, the
discriminative power of procalcitonin could be hampered in these particular patient categories.
A recent study concluded that children with bacterial pneumonia had significantly higher
procalcitonin levels than those with a viral aetiology, but there was a significant degree of
overlap. Procalcitonin has the greatest sensitivity and specificity for differentiating patients with
SIRS from those with sepsis, when compared to IL-2, IL-6, IL-8, CRP and TNF-α. Today
procalcitonin is considered to be one of the earliest and most specific markers of sepsis.
Areas of investigation: Sepsis, Bacterial infection, Septic shock, Inflammation.

Product References (5)

References

  • Iftimie S, García-Heredia A, Pujol I, Ballester F, Fort-Gallifa I, Simó JM,Joven J, Camps J, Castro A. Erratum to: Preliminary study on serum paraoxonase-1 status and chemokine (C-C motif) ligand 2 in hospitalized elderly patients withcatheter-associated asymptomatic bacteriuria. Eur J Clin Microbiol Infect Dis.2016 Sep;35(9):1425. PubMed PMID: 27435069. See more on PubMed
  • Kim SH, Chung JH, Lee JC, Park YH, Joe JH, Hwang HJ. Carboxy-terminalprovasopressin may predict prognosis in nursing home acquired pneumonia. ClinChim Acta. 2013 Jun 5;421:226-9. doi: 10.1016/j.cca.2013.02.022. Epub 2013 Feb27. PubMed PMID: 23454473. See more on PubMed
  • Oncul A, Ates I, Arikan MF, Yilmaz N, Topcuoglu C, Yilmaz FM, Altay M. Therelationship between procalcitonin and thyroid autoantibodies in patients withautoimmune thyroiditis. J Clin Lab Anal. 2017 Nov;31(6). doi: 10.1002/jcla.22123.Epub 2017 Feb 7. PubMed PMID: 28171686. See more on PubMed
  • de Araujo OR, Salomão R, Brunialti MKC, da Silva DCB, Senerchia AA, de Moraes Costa Carlesse FA, Petrilli AS. Cytokine Kinetics in Febrile NeutropenicChildren: Insights on the Usefulness as Sepsis Biomarkers, Influence ofFilgrastim, and Behavior of the IL-23/IL-17 Pathway. Mediators Inflamm.2017;2017:8291316. doi: 10.1155/2017/8291316. Epub 2017 Jul 9. PubMed PMID:28769538; PubMed Central PMCID: PMC5523193. See more on PubMed
  • Tyagi A, Gupta YR, Das S, Rai G, Gupta A. Effect of Segmental ThoracicEpidural Block on Pancreatitisinduced Organ Dysfunction: A Preliminary Study.Indian J Crit Care Med. 2019 Feb;23(2):89-94. doi:10.5005/jp-journals-10071-23123. PubMed PMID: 31086453; PubMed Central PMCID:PMC6487603. See more on PubMed
Summary References (21)

References to Procalcitonin

  • Arkader R, Troster EJ, Lopes MR, Junior RR, Carcillo JA, Leone C, Okay TS. Procalcitonin does discriminate between sepsis and systemic inflammatory response syndrome. Arch Dis Child. 2006 Feb;91 (2):117-20
  • Carrol ED, Thomson AP, Hart CA. Procalcitonin as a marker of sepsis. Int J Antimicrob Agents. 2002 Jul;20 (1):1-9
  • Dumea R, Siriopol D, Hogas S, Mititiuc I, Covic A. Procalcitonin: diagnostic value in systemic infections in chronic kidney disease or renal transplant patients. Int Urol Nephrol. 2014 Feb;46 (2):461-8
  • Floras AN, Holowaychuk MK, Hodgins DC, Marr HS, Birkenheuer A, Sharif S, Bersenas AM, Bienzle D. Investigation of a commercial ELISA for the detection of canine procalcitonin. J Vet Intern Med. 2014 Mar-Apr;28 (2):599-602
  • Giunti M, Peli A, Battilani M, Zacchini S, Militerno G, Otto CM. Evaluation of CALC-I gene (CALCA) expression in tissues of dogs with signs of the systemic inflammatory response syndrome. J Vet Emerg Crit Care (San Ant. 2010 Oct;20 (5):523-7
  • Harbarth S, Holeckova K, Froidevaux C, Pittet D, Ricou B, Grau GE, Vadas L, Pugin J. Diagnostic value of procalcitonin, interleukin-6, and interleukin-8 in critically ill patients admitted with suspected sepsis. Am J Respir Crit Care Med. 2001 Aug 1;164 (3):396-402
  • Kang YA, Kwon SY, Yoon HI, Lee JH, Lee CT. Role of C-reactive protein and procalcitonin in differentiation of tuberculosis from bacterial community acquired pneumonia. Korean J Intern Med. 2009 Dec;24 (4):337-42
  • Kuzi S, Aroch I, Peleg K, Karnieli O, Klement E, Dank G. Canine procalcitonin messenger RNA expression. J Vet Diagn Invest. 2008 Sep;20 (5):629-33
  • Lee H. Procalcitonin as a biomarker of infectious diseases. Korean J Intern Med. 2013 May;28 (3):285-91
  • Lee JY, Hwang SJ, Shim JW, Jung HL, Park MS, Woo HY, Shim JY. Clinical significance of serum procalcitonin in patients with community-acquired lobar pneumonia. Korean J Lab Med. 2010 Aug;30 (4):406-13
  • Maruna P, Nedelnikova K, Gurlich R. Physiology and genetics of procalcitonin. Physiol Res. 2000;49 Suppl 1:S57-61
  • Meisner M. Update on procalcitonin measurements. Ann Lab Med. 2014 Jul;34 (4):263-73
  • Nanda N, Juthani-Mehta M. Novel biomarkers for the diagnosis of urinary tract infection-a systematic review. Biomark Insights. 2009;4:111-21
  • Pecile P, Miorin E, Romanello C, Falleti E, Valent F, Giacomuzzi F, Tenore A. Procalcitonin: a marker of severity of acute pyelonephritis among children. Pediatrics. 2004 Aug;114 (2):e249-54
  • Pourakbari B, Mamishi S, Zafari J, Khairkhah H, Ashtiani MH, Abedini M, Afsharpaiman S, Rad SS. Evaluation of procalcitonin and neopterin level in serum of patients with acute bacterial infection. Braz J Infect Dis. 2010 May-Jun;14 (3):252-5
  • Sand M, Trullen XV, Bechara FG, Pala XF, Sand D, Landgrafe G, Mann B. A prospective bicenter study investigating the diagnostic value of procalcitonin in patients with acute appendicitis. Eur Surg Res. 2009;43 (3):291-7
  • Schneider HG, Lam QT. Procalcitonin for the clinical laboratory: a review. Pathology. 2007 Aug;39 (4):383-90
  • Schuetz P, Albrich W, Mueller B. Procalcitonin for diagnosis of infection and guide to antibiotic decisions: past, present and future. BMC Med. 2011;9:107
  • Schultz MJ, Determann RM. PCT and sTREM-1: the markers of infection in critically ill patients?. Med Sci Monit. 2008 Dec;14 (12):RA241-7
  • Smolkin V, Koren A, Raz R, Colodner R, Sakran W, Halevy R. Procalcitonin as a marker of acute pyelonephritis in infants and children. Pediatr Nephrol. 2002 Jun;17 (6):409-12
  • Summah H, Qu JM. Biomarkers: a definite plus in pneumonia. Mediators Inflamm. 2009;2009:675753
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