Sandwich ELISA, HRP-labelled antibody
serum 50 ul/well
urine 75 ul/well
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Limit of Detection
n = 8; CV = 2.7 %
n = 6; CV = 4.8 %
- bovine Non-detectable
- cat Non-detectable
- goat Non-detectable
- hamster Non-detectable
- horse Non-detectable
- dog Yes
- monkey Non-detectable
- mouse Non-detectable
- pig Non-detectable
- rabbit Non-detectable
- rat Non-detectable
- chicken Not tested
- human Yes
- sheep Yes
- European Union: for in vitro diagnostic use
- Rest of the world: for research use only!
- The total assay time is less than 4 hours
- The kit measures procalcitonin in serum and urine
- Assay format is 96 wells
- Standard is recombinant protein based
- Quality Controls are human serum based
- Components of the kit are provided ready to use, concentrated or lyophilized
Immune Response, Infection and Inflammation, Sepsis
Procalcitonin (PCT) the precursor of the hormone calcitonin is a 116 amino acid protein with a
molecular mass of 13 kDa. It undergoes successive cleavages in the neuroendocrine cells of
the thyroid to form three distinct molecules: calcitonin (32 amino acids); katacalcin (21 amino
acids) and N-terminal fragment called aminoprocalcitonin (57 amino acids). Procalcitonin
belongs to a group of related proteins including calcitonin gene-related peptides I and II,
amylin, adrenomodulin and calcitonin (CAPA peptide family). Synthesis of procalcitonin is
regulated gene CALC-1. Under normal metabolic conditions procalcitonin is present in the
C-cells of the thyroid gland. The level of procalcitonin in the blood of healthy individuals is low.
The risk of local bacterial infection occurs when the value of procalcitonin exceeds 0.25 ng/ml.
The risk of systemic bacterial infection occurs when the value of procalcitonin exceeds 0.5
ng/ml. Bacterial lipopolysacharide (LPS) has been shown to be a potent inducer of
procalcitonin release into systemic circulation. This release is not associated with an increase
in calcitonin. Procalcitonin levels increase from 3 to 4 hours, peak at about 6 hours and then
plateau for up to 24 hours. In contrast, C-reactive protein (CRP) levels rise between 12 and 18
hours after bacterial challenge. In blood serum, procalcitonin has a half-life of between 25 and
30 hours. A study showed that hepatocytes produce large amounts of procalcitonin following
stimulation with TNF-α and IL-6. In acute pancreatitis, procalcitonin closely correlates with the
development of pancreatic infections. Since procalcitonin has been reported to be increased in
different non-septic conditions such as major trauma, acute respiratory distress syndrome,
rejection after transplantation, cardiogenic shock, severe burns and heat-stroke, the
discriminative power of procalcitonin could be hampered in these particular patient categories.
A recent study concluded that children with bacterial pneumonia had significantly higher
procalcitonin levels than those with a viral aetiology, but there was a significant degree of
overlap. Procalcitonin has the greatest sensitivity and specificity for differentiating patients with
SIRS from those with sepsis, when compared to IL-2, IL-6, IL-8, CRP and TNF-α. Today
procalcitonin is considered to be one of the earliest and most specific markers of sepsis.
Areas of investigation: Sepsis, Bacterial infection, Septic shock, Inflammation.