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Manufactured by BioVendor

Prolactin Human E. coli

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Species:Human
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Cat. No. Size Price


RP1762670010 10 µg
RP1762670050 50 µg
RP1762671000 1 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Prolactin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton. The Prolactin is purified by proprietary chromatographic techniques.

Amino Acid Sequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeMet-Leu-Pro-Ile-Cys

Source

E. coli

Purity

Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2–11 was found to be < 0.065 ng/ml.

Formulation

The protein was lyophilized from a concentrated (1 mg/ml) solution with 10 mM sodium Phosphate buffer pH=8 and 50 mM NaCl.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution Prolactin should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Summary

Summary

Prolactin (about 24 kDa, 199 amino acids) is a peptide hormone primarily associated with lactation. It is synthesised and secreted by lactotrope cells in the adenohypophysis. It is also produced in other tissues including the breast and the decidua. Prolactin stimulates the mammary glands to produce milk (lactation).

Summary References (25)

References to Prolactin

  • Allolio B, Hoeppener A, Leonhardt U, Deuss U, Winkelmann W. Size heterogeneity of immunoreactive prolactin in patients with prolactinoma. Acta Endocrinol (Copenh). 1987 Apr;114 (4):475-82
  • Aubert ML, Grumbach MM, Kaplan SL. Heterologous radioimmunoassay for plasma human prolactin (hPRL); values in normal subjects, puberty, pregnancy and in pituitary disorders. Acta Endocrinol (Copenh). 1974 Nov;77 (3):460-76
  • Bonhoff A, Vuille JC, Gomez F, Gellersen B. Identification of macroprolactin in a patient with asymptomatic hyperprolactinemia as a stable PRL-IgG complex. Exp Clin Endocrinol Diabetes. 1995;103 (4):252-5
  • Cavaco B, Leite V, Santos MA, Arranhado E, Sobrinho LG. Some forms of big big prolactin behave as a complex of monomeric prolactin with an immunoglobulin G in patients with macroprolactinemia or prolactinoma. J Clin Endocrinol Metab. 1995 Aug;80 (8):2342-6
  • Cowden EA, Ratcliffe WA, Beastall GH, Ratcliffe JG. Laboratory assessment of prolactin status. Ann Clin Biochem. 1979 May;16 (3):113-21
  • Fahie-Wilson MN. Detection of macroprolactin causing hyperprolactinemia in commercial assays for prolactin. Clin Chem. 2000 Dec;46 (12):2022-3
  • Forsyth IA, Myres RP. Human prolactin. Evidence obtained by the bioassay of human plasma. J Endocrinol. 1971 Sep;51 (1):157-68
  • Frantz AG. Prolactin. N Engl J Med. 1978 Jan 26;298 (4):201-7
  • Friesen H, Guyda H, Hardy J. The biosynthesis of human growth hormone and prolactin. J Clin Endocrinol Metab. 1970 Dec;31 (6):611-24
  • Friesen H, Guyda H, Hwang P. 2,3-diphosphoglycerate affects enzymes of glucose metabolism in red blood cells. Nat New Biol. 1971 Jul 7;232 (27):19-20
  • Friesen H, Hwang P. Human prolactin. Annu Rev Med. 1973;24:251-70
  • Hwang P, Guyda H, Friesen H. A radioimmunoassay for human prolactin. Proc Natl Acad Sci U S A. 1971 Aug;68 (8):1902-6
  • Hwang P, Guyda H, Friesen H. Purification of human prolactin. J Biol Chem. 1972 Apr 10;247 (7):1955-8
  • Jacobs LS, Snyder PJ, Wilber JF, Utiger RD, Daughaday WH. Increased serum prolactin after administration of synthetic thyrotropin releasing hormone (TRH) in man. J Clin Endocrinol Metab. 1971 Dec;33 (6):996-8
  • Kelly PA, Djiane J, Katoh M, Ferland LH, Houdebine LM, Teyssot B, Dusanter-Fourt I. The interaction of prolactin with its receptors in target tissues and its mechanism of action. Recent Prog Horm Res. 1984;40:379-439
  • Kelly PA, Posner BI, Tsushima T, Friesen HG. Studies of insulin, growth hormone and prolactin binding: ontogenesis, effects of sex and pregnancy. Endocrinology. 1974 Aug;95 (2):532-9
  • Kleinberg DL, Frantz AG. Human prolactin: measurement in plasma by in vitro bioassay. J Clin Invest. 1971 Aug;50 (8):1557-68
  • Lewis UJ, Singh RN, Seavey BK. Human prolactin: isolation and some properties. Biochem Biophys Res Commun. 1971 Sep;44 (5):1169-76
  • Linzer DI, Nathans D. Nucleotide sequence of a growth-related mRNA encoding a member of the prolactin-growth hormone family. Proc Natl Acad Sci U S A. 1984 Jul;81 (14):4255-9
  • McLeod. RM. Regulation of Prolactin Secretion: In Frontiers in Neuroendocrinology (Martin L. and Garrong N.F. eds). Raven Press. 164,1976;
  • Nikolics K, Mason AJ, Szonyi E, Ramachandran J, Seeburg PH. A prolactin-inhibiting factor within the precursor for human gonadotropin-releasing hormone. Nature. 1985 Aug 8-14;316 (6028):511-7
  • Rasmussen C, Bergh T, Wide L, Brownell J. CV 205-502: a new long-acting drug for inhibition of prolactin hypersecretion. Clin Endocrinol (Oxf). 1987 Mar;26 (3):321-6
  • Reyes FI, Winter JS, Faiman C. Pituitary-ovarian relationships preceding the menopause. I. A cross-sectional study of serum follice-stimulating hormone, luteinizing hormone, prolactin, estradiol, and progesterone levels. Am J Obstet Gynecol. 1977 Nov 1;129 (5):557-64
  • Schneider W, Marcovitz S, Al-Shammari S, Yago S, Chevalier S. Reactivity of macroprolactin in common automated immunoassays. Clin Biochem. 2001 Sep;34 (6):469-73
  • Shiu RP, Iwasiow BM. Prolactin-inducible proteins
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