Prolactin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 200 amino acids and having a molecular mass of 23007 Dalton. The Prolactin is purified by proprietary chromatographic techniques.
Amino Acid Sequence
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
The ED50 as determined by the dose-dependant stimulation of the proliferation of rat lymphoma, Nb2–11 was found to be < 0.065 ng/ml.
The protein was lyophilized from a concentrated (1 mg/ml) solution with 10 mM sodium Phosphate buffer pH=8 and 50 mM NaCl.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Lyophilized Prolactin although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution Prolactin should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Sterile filtered white lyophilized (freeze-dried) powder.