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Manufactured by BioVendor

Proteinase 3 (PR3) Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Human
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Cat. No. Size Price


RAI001R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Cell culture medium, Plasma

Sample Requirements

10 µl/sample

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

2–8°C

Calibration Curve

Calibration Range

0.156 – 10 ng/ml

Summary

Research topic

Immune Response, Infection and Inflammation, Oncology

Summary

Proteinase 3 (PR3), also known as myeloblastin, Wegener autoantigen, PRTN3 and NP- 4, is one of the hematopoietic serine proteases localized in the primary granules of polymorphonuclear neutrophils (PMNs). The primary function of PR3 is recognized as to participate in direct intracellular killing of phagocytosed pathogens in phagolysosomes and degradation of extracellular matrix components at inflammatory sites. PR3 has also been proven to be able to process some pro-inflammatory cytokines, such as IL-1β, IL-18 and TNF-α, activate mitogen activated protein kinase (MAPK) signaling through proteinase activated receptor-1 (PAR1), and induce endothelial cell apoptosis through NF-κB signaling pathways. PR3 is identified as the target autoantigen of anti-neutrophil cytoplasmic autoantibodies (ANCA) in Wegener granulomatosis 6. Increased PR3 levels have been reported in patients with acute myocardial infarction, and in subjects with type 1 diabetes.

References to Product

References

  • Korkmaz, B., Moreau, T. & Gauthier, F. Neutrophil elastase, proteinase 3 and cathepsin G: Physicochemical properties, activity and physiopathological functions. Biochimie 90, 227-242 (2008).
  • Mihara, K., Ramachandran, R., Renaux, B., Saifeddine, M. & Hollenberg, M.D. Neutrophil elastase and proteinase-3 trigger G-protein biased signaling through proteinase activated receptor-1 (PAR1). J Biol Chem (2013).
  • Nauseef, W.M. & Borregaard, N. Neutrophils at work. Nature immunology 15, 602-611 (2014).
  • Ng, L.L., et al. Proteinase 3 and prognosis of patients with acute myocardial infarction. Clinical science 120, 231-238 (2011).
  • Pham, C.T. Neutrophil serine proteases: specific regulators of inflammation. Nat Rev Immunol 6, 541-550 (2006).
  • van der Geld, Y.M., Limburg, P.C. & Kallenberg, C.G. Proteinase 3, Wegener's autoantigen: from gene to antigen. J Leukoc Biol 69, 177-190 (2001).
  • Wiedow, O. & Meyer-Hoffert, U. Neutrophil serine proteases: potential key regulators of cell signalling during inflammation. J Intern Med 257, 319-328 (2005).
  • Wang, Y., et al. Increased Neutrophil Elastase and Proteinase 3 and Augmented NETosis Are Closely Associated with beta-cell Autoimmunity in Patients with Type 1 Diabetes. Diabetes (2014).
References to Summary

References to Proteinase-3

  • Korkmaz B, Moreau T, Gauthier F. Neutrophil elastase, proteinase 3 and cathepsin G: physicochemical properties, activity and physiopathological functions. Biochimie. 2008 Feb;90 (2):227-42
  • Mihara K, Ramachandran R, Renaux B, Saifeddine M, Hollenberg MD. Neutrophil elastase and proteinase-3 trigger G protein-biased signaling through proteinase-activated receptor-1 (PAR1). J Biol Chem. 2013 Nov 15;288 (46):32979-90
  • Nauseef WM, Borregaard N. Neutrophils at work. Nat Immunol. 2014 Jul;15 (7):602-11
  • Ng LL, Khan SQ, Narayan H, Quinn P, Squire IB, Davies JE. Proteinase 3 and prognosis of patients with acute myocardial infarction. Clin Sci (Lond). 2011 Mar;120 (6):231-8
  • Pham CT. Neutrophil serine proteases: specific regulators of inflammation. Nat Rev Immunol. 2006 Jul;6 (7):541-50
  • van der Geld YM, Limburg PC, Kallenberg CG. Proteinase 3, Wegener's autoantigen: from gene to antigen. J Leukoc Biol. 2001 Feb;69 (2):177-90
  • Wang Y, Xiao Y, Zhong L, Ye D, Zhang J, Tu Y, Bornstein SR, Zhou Z, Lam KS, Xu A. Increased Neutrophil Elastase and Proteinase 3 and Augmented NETosis Are Closely Associated with beta-cell Autoimmunity in Patients with Type 1 Diabetes. Diabetes. 2014 Aug 4;
  • Wiedow O, Meyer-Hoffert U. Neutrophil serine proteases: potential key regulators of cell signalling during inflammation. J Intern Med. 2005 Apr;257 (4):319-28
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