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Purple-Jelley Hyaluronan Assay (Large Economy Kit)

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Species:Multispecies
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H2000 400 assays (1 kit)
PubMed Product Details
Technical Data

Cat # changed from RBCH2000 to H2000

Type

Colorimetric assay

Applications

Tissue extract

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at RT or 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Limit of Detection

0.2 μg

Summary

Features

  • The Purple-Jelley Hyaluronan Assay is used to measure the quantity of hyaluronan (hyaluronic acid) in mammalian tissue. It is a dye-based method with a detection limit of 0.2μg.

Research topic

Extracellular matrix, Animal studies

Summary

Hyaluronic acid (HA), also known as hyaluronan, is a non-sulphated glycosaminoglycan (GAG) and is composed of repeating polymeric disaccharides of D-glucuronic acid and N-acetyl-D-glucosamine linked by a glucuronidic β (1→3) bond. In aqueous solutions HA forms specific stable tertiary structures. Despite the simplicity in its composition, without variations in its sugar composition or without branching points, HA has a variety of physicochemical properties. HA polymers occur in a vast number of configurations and shapes, depending on their size, salt concentration, pH, and associated cations. Unlike other GAG, HA is not covalently attached to a protein core, but it may form aggregates with proteoglycans. HA encompasses a large volume of water giving solutions high viscosity, even at low concentrations.

HA is widely distributed, from prokaryotic, to eukaryotic cells. In humans, HA is most abundant in the skin, (accounting for 50% of the total body HA), the vitreous of the eye, the umbilical cord, and synovial fluid, but it is also present in all tissues and fluids of the body, such as skeletal tissues, heart valves, the lung, the aorta, the prostate, tunica albuginea, corpora cavernosa and corpus spongiosum of the penis. HA is produced primarily by mesenchymal cells but also by other cell types.

Considerable evidence exists regarding the functional role of HA in molecular mechanisms and, consequently, the potential role of HA for the development of novel therapeutic strategies for many diseases.

Functions of HA include the following: hydration, lubrication of joints, a space filling capacity, and the framework through which cells migrate. The synthesis of HA increases during tissue injury and wound healing and HA regulates several aspects of tissue repair, including activation of inflammatory cells to enhance immune response and the response to injury of fibroblasts and epithelial cells. HA also provides the framework for blood vessel formation and fibroblast migration, that may be involved in tumor progression. The correlation of HA levels on the cell surface of cancer cells with the aggressiveness of tumors has also been reported.

The size of HA appears to be of critical importance for its various functions described above. HA of high molecular size, usually in excess of 1,000 kDa, is present in intact tissues and is antiangiogenic and immunosuppressive, whereas smaller polymers of HA are distress signals and potent inducers of inflammation and angiogenesis.

Summary References (47)

References to Hyaluronan

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