Cat # changed from RSCYK080R to YK080
Type
Competitive ELISA
Description
This EIA kit is used for quantitative determination of human PYY (both PYY(3-36) and PYY(1-36)) in
serum and plasma samples. The kit is characterized by its sensitive quantification and high specificity. In
addition, it has no influence by other components i n samples. Human PYY (3 -36) standard is highly
purified synthetic product.
Applications
Serum, Plasma
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
0.082–20 ng/ml
Intra-assay (Within-Run)
serum 3.67-5.13 %, plasma 6.08-8.52 %
Inter-assay (Run-to-Run)
serum 2.33-6.55 %, plasma 5.45-10.26 %
Spiking Recovery
Human serum 79,7 - 110,2 %
Human plasma 78,9 - 105,2 %
Specificity
Th
e EIA kit shows 100 % cross reactivity to human PYY (3-36) and human PYY (1-36), and shows less
than 0.003 % cross reactivity to human and rat NPY, which have similar amino acid sequence with human
PYY.
Features
- RUO
- assay range : 0.082-20 ng/ml
- the assay is completed within 16-18 hr.+13 hr.
- With one assay kit, 41 samples can be measured in duplicate
Research topic
Energy metabolism and body weight regulation
Summary
This enzyme immunoassay(EIA) kit is a stable and convenient assay system for human peptide YY
(PYY). PYY was isolated initially by Tatemoto et al. (1980 ) from the extract of pig duodenum and shown
to be a polypeptide consisting of 36 amino acids residues. PYY is homologous to pancreatic polypeptide
(PP) and neuropeptide Y (NPY). PYY is localized mainly in endocrine cells in the intestine (ileum, colon,
and rectum). PYY shows a n inhibitory action on contraction of the gastrointestinal tract and on secretion of
pancreatic and gastric juice. PYY is released by taking diet. The PYY level in human blood decreases after
resection of the intestine, possibly be due to the decrease in n umber of the endocrine cells secreting PYY.
The EIA kit is prepared by using synthetic human PYY (3-36) as standard and biotinylated human PYY(3 -36) as labeled antigen. The kit can be used for measurement of PYY (both PYY(3-36) and PYY(1-36)) in human ser um or plasma with high sensitivity. It will be a specifically useful tool for PYY research.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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