Measure all types of fibrillary collagen in a fast and simple 96-well plate format!
Collagen is one of the main components of extracellular matrix. Dysregulation in collagen production results in pathologies such as fibrosis (too much collagen), or osteoarthritis (too little collagen). Therefore measurement of collagen production is important in many disease related studies.
The QuickZyme collagen assay is based on binding of the dye Sirius Red to collagen. Following binding of the dye, the collagen-dye complex precipitates, resulting in a coloured pellet. This colour can be released in an alkaline solution.
The assay measures various types of fibrillar collagen. The most abundant collagen types are collagen type I (skin, bone, vessels, connective tissue), type II (cartilage) and type III (skin, bone, connective tissue).
The assay is specific for collagen. After heating at 50°C the signal is lost, since the assay does not recognize denatured collagen (gelatin). The assay can be used to analyze soluble collagen or collagen that is solubilized by acetic acid alone or in combination with pepsin. The assay is intended for collagen analysis in conditioned cell culture media and cellular extracts. The assay is not affected by serum up to at least 10%.
Cellular extracts, Tissue homogenates, Tissue culture supernatants
10 - 140 µl
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Unopened kit: Store at 4°C. Do not use kit components past kit expiration date.
Opened kit / reconstituted reagents:
Collagen standard dissolved in 20 mM HAc: store at 4°C up to 1 month
Dye solution: store at 4°C up to 1 month
Dilution buffer: store at -20°C up to 6 months
Wash buffer: store at -20°C up to 6 months
Detection solution: store either at 4°C or room temperature up to 1 month
0 - 10 µg/well
Limit of Detection