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RatLaps™ ELISA (CTX-I)

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Other names:C-terminal telopeptide I, carboxy-terminal collagen I crosslinks
  • Species:Mouse, Rat
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Cat. No. Size Price


AC-06F1 96 wells
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Description

RatLaps™ (CTX-I) EIA is an enzyme-linked immunosorbent assay for the quantitative determination of bone-related degradation products from C-terminal telopeptides of type I collagen in rat/mouse serum or urine, and from rat/mouse bone released into cell culture supernatants by osteoclasts. The assay is for research use only.

Scientific Description:

Type I collagen accounts for more than 90% of the organic matrix of bone and is synthesised primary in bone. During renewal of the skeleton bone matrix is degraded and consequently fragments of type I collagen are released into circulation. The RatLaps™ (CTX-I) EIA is based on the observation that certain C-telopeptide degradation products from type I collagen released during osteoclastic bone resorption. The RatLaps™ (CTX-I) EIA is based upon the competitive binding of a polyclonal antibody to soluble RatLaps antigens EKSQDGGR or to immobilised RatLaps antigens. Briefly, the polyclonal antibody is raised against a synthetic peptide having a sequence (EKSQDGGR) specific for a part of the C-terminal telopeptide α1 chain of rat type I collagen. For standardisation of the RatLaps™ (CTX-I) EIA a synthetic peptide (EKSQDGGR) that is specific for the C-terminal telopeptide α1 chain of type I collagen in rats has been used.

Applications

Serum, Urine, Plasma, Cell culture supernatant

Sample Requirements

20 μL/well

Storage/Expiration

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

0-200 ng/mL

Limit of Detection

2.0 ng/mL

Summary

Features

  • Assessment of Bone Resorption in Rodents
  • Ovariectomised (OVX) rats and mice
  • Thyroidparathyroidectomised (TPTX) rats
  • Rat and mouse models of bone metastases
  • Knock-out and transgenic mice with effect in bone resorption
  • Assessment of Bone Resorption in Tissue Culture
  • Calvariae, tibiae, or metatarsals from rats and mice
  • Multi-disciplinary use -measuring urine and serum samples
  • All reagents ready for use

Research topic

Bone and cartilage metabolism, Animal studies

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