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Rat/Mouse PINP EIA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Other names:aminoterminal propeptide of type I procollagen
  • Species:Mouse, Rat
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Cat. No. Size Price


AC-33F1 96 wells
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Description

The Immunodiagnostic Systems Limited Rat/Mouse PINP EIA kit is a competitive enzyme immunoassay (EIA) for the quantitative determination of N-terminal propeptide of type I procollagen (PINP) in rat or mouse serum, plasma (Heparin or EDTA), or cell culture media samples. PINP is a particularly useful marker for monitoring the efficacy of osteoporosis therapy with anabolic agents, but it is also one of the best bone turnover markers for monitoring the efficacy of antiresorptive therapy.

Scientific Description:

The Rat/Mouse PINP EIA assay is a specific method to determine PINP released during rat and mouse bone collagen synthesis, and it has no cross-reactivity for human PINP. The Rat/Mouse PINP EIA assay can be used for determining the bone formation rate from rat and mouse serum samples, and from the culture medium in rat and mouse osteoblast cultures. Previous studies have demonstrated that PINP secretion is increased after stimulation of mouse osteoblasts by BMP-4 and oestrogen in vitro. PINP values are also elevated after treatment with PTH in both young ovariectomised rats and old intact rats.

Applications

Serum, Cell culture medium, Plasma, Plasma (EDTA, citrate, heparin)

Sample Requirements

5 μL, predilution 1:10

Storage/Expiration

Store the complete kit at 2-8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

0.7 – 75 ng/mL

Summary

Features

  • Unique – the first commercially available EIA to quantify type I collagen formation in rat or mouse specimens:
  • Ideal for rodents – only 5μL per well
  • Flexible – serum or plasma samples can be used
  • Fast turnaround time – results in 3 hours
  • Complementary – use in conjunction with IDS RatTRAPTM
  • MouseTRAPTM or RatLapsTM to evaluate both components of bone remodeling
  • A complete assay panel supporting bone research
  • Low sample volume – only 5μL per well
    -Multi-disciplinary use – measuring plasma and serum samples

Research topic

Bone and cartilage metabolism, Animal studies

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