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sAPO-1/Fas Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:CD95, Fas receptor, APT 1, ALPS 1 A, TNFRSF6
  • Species:Human
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Cat. No. Size Price


RAF085R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cell culture supernatant

Sample Requirements

10 µl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

15.6–1000 pg/ml

Limit of Detection

13.2 pg/ml

Intra-assay (Within-Run)

CV = 4.5%

Inter-assay (Run-to-Run)

CV = 3.1%

Spiking Recovery

100,00%

Dilutation Linearity

102,00%

Summary

Research topic

Apoptosis

Summary

Programmed cell death or apoptosis is the most common form of eukaryotic cell death and is found during tumor regression and embryonic development. In the process of selection and eliminiation of autoreactive B and T cells apoptosis is an important process and therefore a prerequisite for the homeostasis of the immune system. Apoptosis is characterized by changes in cellular morphology (e.g. nuclear condensation, membrane bleeding) and biochemically by rapid induction on DNA fragmentation. APO-1/Fas (CD95), a member of the TNF/NGF receptor superfamily, is a glycosylated 48 kDa surface protein containing a single transmembrane region. APO-1 is expressed on a variety of human B and T cell lines, on many different tumor cells and on various normal human tissues. Triggering of APO-1 by its ligand or by certain anti-APO-1 monoclonal antibodies
results in rapid induction of programmed cell death, apoptosis, in susceptible cells. The tissue distribution of APO-1 and of the APO-1 ligand suggests that the APO-1 receptor/ligand system plays an important role in various aspects of mammalian development and especially in the homeostasis of the immune system.
Expression of the APO-1 cell surface protein is enhanced by IFN-g and TNF and by activation in lymphocytes. APO-1 also occurs in a soluble form (sAPO-1) devoid of a transmembrane region. Elevated sAPO-1 levels have been reported in sera from patients with high-and low-grade malignant B- and T-cell leukemias and systemic lupus erythematosus. sAPO-1 may prevent cells from undergoing APO-1 ligand induced apoptosis. Hence, secretion of sAPO-1 may provide a mechanism for tumor cells to excape immunosurveillance and may be involved
in leukemogenesis.

References to Summary

References to APO-1/Fas

  • Cheng J, Zhou T, Liu C, Shapiro JP, Brauer MJ, Kiefer MC, Barr PJ, Mountz JD. Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule. Science. 1994 Mar 25;263 (5154):1759-62
  • Cheng J, Zhou T, Liu C, Shapiro JP, Brauer MJ, Kiefer MC, Barr PJ, Mountz JD. Protection from Fas-mediated apoptosis by a soluble form of the Fas molecule. Science. 1994 Mar 25;263 (5154):1759-62
  • Daniel PT, Krammer PH. Activation induces sensitivity toward APO-1 (CD95)-mediated apoptosis in human B cells. J Immunol. 1994 Jun 15;152 (12):5624-32
  • Daniel PT, Krammer PH. Activation induces sensitivity toward APO-1 (CD95)-mediated apoptosis in human B cells. J Immunol. 1994 Jun 15;152 (12):5624-32
  • Debatin KM, Goldmann CK, Bamford R, Waldmann TA, Krammer PH. Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia. Lancet. 1990 Mar 3;335 (8688):497-500
  • Debatin KM, Goldmann CK, Bamford R, Waldmann TA, Krammer PH. Monoclonal-antibody-mediated apoptosis in adult T-cell leukaemia. Lancet. 1990 Mar 3;335 (8688):497-500
  • Itoh N, Yonehara S, Ishii A, Yonehara M, Mizushima S, Sameshima M, Hase A, Seto Y, Nagata S. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 1991 Jul 26;66 (2):233-43
  • Itoh N, Yonehara S, Ishii A, Yonehara M, Mizushima S, Sameshima M, Hase A, Seto Y, Nagata S. The polypeptide encoded by the cDNA for human cell surface antigen Fas can mediate apoptosis. Cell. 1991 Jul 26;66 (2):233-43
  • Klas C, Debatin KM, Jonker RR, Krammer PH. Activation interferes with the APO-1 pathway in mature human T cells. Int Immunol. 1993 Jun;5 (6):625-30
  • Klas C, Debatin KM, Jonker RR, Krammer PH. Activation interferes with the APO-1 pathway in mature human T cells. Int Immunol. 1993 Jun;5 (6):625-30
  • Knipping E, Debatin KM, Stricker K, Heilig B, Eder A, Krammer PH. Identification of soluble APO-1 in supernatants of human B- and T-cell lines and increased serum levels in B- and T-cell leukemias. Blood. 1995 Mar 15;85 (6):1562-9
  • Knipping E, Debatin KM, Stricker K, Heilig B, Eder A, Krammer PH. Identification of soluble APO-1 in supernatants of human B- and T-cell lines and increased serum levels in B- and T-cell leukemias. Blood. 1995 Mar 15;85 (6):1562-9
  • Leithauser F, Dhein J, Mechtersheimer G, Koretz K, Bruderlein S, Henne C, Schmidt A, Debatin KM, Krammer PH, Moller P. Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells. Lab Invest. 1993 Oct;69 (4):415-29
  • Leithauser F, Dhein J, Mechtersheimer G, Koretz K, Bruderlein S, Henne C, Schmidt A, Debatin KM, Krammer PH, Moller P. Constitutive and induced expression of APO-1, a new member of the nerve growth factor/tumor necrosis factor receptor superfamily, in normal and neoplastic cells. Lab Invest. 1993 Oct;69 (4):415-29
  • Moller P, Henne C, Leithauser F, Eichelmann A, Schmidt A, Bruderlein S, Dhein J, Krammer PH. Coregulation of the APO-1 antigen with intercellular adhesion molecule-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells and in follicle center and mediastinal B-cell lymphomas. Blood. 1993 Apr 15;81 (8):2067-75
  • Moller P, Henne C, Leithauser F, Eichelmann A, Schmidt A, Bruderlein S, Dhein J, Krammer PH. Coregulation of the APO-1 antigen with intercellular adhesion molecule-1 (CD54) in tonsillar B cells and coordinate expression in follicular center B cells and in follicle center and mediastinal B-cell lymphomas. Blood. 1993 Apr 15;81 (8):2067-75
  • Munker R, Lubbert M, Yonehara S, Tuchnitz A, Mertelsmann R, Wilmanns W. Expression of the Fas antigen on primary human leukemia cells. Ann Hematol. 1995 Jan;70 (1):15-7
  • Munker R, Lubbert M, Yonehara S, Tuchnitz A, Mertelsmann R, Wilmanns W. Expression of the Fas antigen on primary human leukemia cells. Ann Hematol. 1995 Jan;70 (1):15-7
  • Oehm A, Behrmann I, Falk W, Pawlita M, Maier G, Klas C, Li-Weber M, Richar
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