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sCD40L Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:CD40-L, CD40 ligand, Tumor necrosis factor ligand superfamily member 5, TNF related activation protein, T cell antigen Gp39, CD154 antigen
  • Species:Human
Cat. No. Size Price

RAF093R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


Serum, Plasma, Cell culture supernatant

Sample Requirements

20 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Calibration Curve

Calibration Range

0.16–10 ng/ml

Limit of Detection

0.06 ng/ml

Intra-assay (Within-Run)

CV = 4.0%

Inter-assay (Run-to-Run)

CV = 6.8%

Spiking Recovery


Dilution Linearity



Research topic

Apoptosis, Cardiovascular disease, Cell surface proteins (sCD), Cytokines and chemokines and related molecules, Transplantation


CD40 belongs to the TNF receptor superfamily. A key role of CD40/CD40ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. This molecule as well as the other ligands of the family share the property of co-stimulation of T-cell proliferation and are all expressed by activated T-cells.Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T-helper cells. Thus the CD40/CD40L interaction plays a central role in the various phases of the B cell response to T-dependent antigens.

Product References (10)


  • Rathmell JC, Townsend SE, Xu JC, Flavell RA, Goodnow CC. Expansion or elimination of B cells in vivo: dual roles for CD40- and Fas (CD95)-ligands modulated by the B cell antigen receptor. Cell. 1996 Oct 18;87(2):319-29. doi: 10.1016/s0092-8674(00)81349-5. PubMed PMID: 8861915. See more on PubMed
  • Bergman MC, Attrep JF, Grammer AC, Lipsky PE. Ligation of CD40 influences the function of human Ig-secreting B cell hybridomas both positively and negatively. J Immunol. 1996 May 1;156(9):3118-32. PubMed PMID: 8617932. See more on PubMed
  • Brugnoni D, Rossi G, Tucci A, Cattaneo R, Airò P. Study of CD40 ligand expression in B-cell chronic lymphocytic leukemia. Haematologica. 1995 Sep-Oct;80(5):440-2. PubMed PMID: 8566887. See more on PubMed
  • Mazzei GJ, Edgerton MD, Losberger C, Lecoanet-Henchoz S, Graber P, Durandy A, Gauchat JF, Bernard A, Allet B, Bonnefoy JY. Recombinant soluble trimeric CD40 ligand is biologically active. J Biol Chem. 1995 Mar 31;270(13):7025-8. doi: 10.1074/jbc.270.13.7025. PubMed PMID: 7706236. See more on PubMed
  • Rothstein TL, Wang JK, Panka DJ, Foote LC, Wang Z, Stanger B, Cui H, Ju ST, Marshak-Rothstein A. Protection against Fas-dependent Th1-mediated apoptosis by antigen receptor engagement in B cells. Nature. 1995 Mar 9;374(6518):163-5. doi: 10.1038/374163a0. PubMed PMID: 7533263. See more on PubMed
  • Cosman D. A family of ligands for the TNF receptor superfamily. Stem Cells. 1994 Sep;12(5):440-55. doi: 10.1002/stem.5530120501. PubMed PMID: 7528588. See more on PubMed
  • Tsubata T, Murakami M, Nisitani S, Honjo T. Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice. Philos Trans R Soc Lond B Biol Sci. 1994 Aug 30;345(1313):297-301. doi: 10.1098/rstb.1994.0109. PubMed PMID: 7531346. See more on PubMed
  • Durie FH, Fava RA, Foy TM, Aruffo A, Ledbetter JA, Noelle RJ. Prevention of collagen-induced arthritis with an antibody to gp39, the ligand for CD40. Science. 1993 Sep 3;261(5126):1328-30. doi: 10.1126/science.7689748. PubMed PMID: 7689748. See more on PubMed
  • Holder MJ, Wang H, Milner AE, Casamayor M, Armitage R, Spriggs MK, Fanslow WC, MacLennan IC, Gregory CD, Gordon J, et al.. Suppression of apoptosis in normal and neoplastic human B lymphocytes by CD40 ligand is independent of Bc1-2 induction. Eur J Immunol. 1993 Sep;23(9):2368-71. doi: 10.1002/eji.1830230948. PubMed PMID: 8103750. See more on PubMed
  • Lane P, Brocker T, Hubele S, Padovan E, Lanzavecchia A, McConnell F. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J Exp Med. 1993 Apr 1;177(4):1209-13. doi: 10.1084/jem.177.4.1209. PubMed PMID: 7681469. PubMed CentralPMCID: PMC2190964. See more on PubMed
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