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sCD40L Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Mouse
  • Species:Mouse
Cat. No. Size Price

RAF094R 96 wells (1 kit) $694,28
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


Serum, Cell culture supernatant

Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.31–20 ng/ml

Limit of Detection

0.14 ng/ml

Intra-assay (Within-Run)

CV = 6.5%

Inter-assay (Run-to-Run)

CV = 11.1%

Spiking Recovery


Dilution Linearity



Research topic

Apoptosis, Autoimmunity, Cardiovascular disease, Oncology, Animal studies


CD40 belongs to the TNF receptor superfamily. While the biological role of some of the ligand-receptor pairs in this family still remains obscure, CD40 has proven its importance.
A key role of CD40/CD40 ligand interactions in immune activation, particularly in T-cell dependent B cell responses is anticipated. This molecule as well as the other ligands of the family share the property of co-stimulation of T-cell proliferation and are all expressed by activated T-cells.
The programmed cell death has been suggested to be involved in clonal elimination of self-reactive lymphocytes for the normal function of the immune system. Interaction with membrane bound self antigens may eliminate self-reactive nature B cells by apoptosis. Antigen-receptor mediated B cell apoptosis is blocked when a signal is transduced via the CD40 molecule on the B cell surface.
Because the ligand of CD40 (CD40L) is expressed on activated T helper cells, B cells may escape from apoptosis and are activated when the immune system interacts with foreign antigens, which are normally able to activate T-helper cells. Thus the CD40 - CD40L interaction plays a central role in the various phases of the B cell response to T-dependent antigens.
Taken together, B cells can participate in regulating their own destruction. Protection against Fas-dependent apoptosis afforded by immunoglobulin-receptor engagement may constitute a fail-safe mechanism that eliminates bystander B cells activated by CD40L - expressing T cells, but ensures survival of antigen-specific B cells.
CD40 Ligand is expressed on the surface of activated CD4+ T cells, basophils, and mast cells. Binding of CD40L to its receptor, CD40, on the surface of B cells stimulates B-cell proliferation, adhesion and differentiation. A soluble isoform of CD40L has been shown to exist in the circulation. This soluble molecule is a homotrimer of a 18kDa protein exhibiting full activity in B cell proliferation and differentiation assays, is able to rescue B cells from apoptosis and binds soluble CD40.

Product References (6)


  • Rathmell JC, Townsend SE, Xu JC, Flavell RA, Goodnow CC. Expansion or elimination of B cells in vivo: dual roles for CD40- and Fas (CD95)-ligands modulated by the B cell antigen receptor. Cell. 1996 Oct 18;87(2):319-29. doi: 10.1016/s0092-8674(00)81349-5. PubMed PMID: 8861915. See more on PubMed
  • Rothstein TL, Wang JK, Panka DJ, Foote LC, Wang Z, Stanger B, Cui H, Ju ST, Marshak-Rothstein A. Protection against Fas-dependent Th1-mediated apoptosis by antigen receptor engagement in B cells. Nature. 1995 Mar 9;374(6518):163-5. doi: 10.1038/374163a0. PubMed PMID: 7533263. See more on PubMed
  • Cosman D. A family of ligands for the TNF receptor superfamily. Stem Cells. 1994 Sep;12(5):440-55. doi: 10.1002/stem.5530120501. PubMed PMID: 7528588. See more on PubMed
  • Tsubata T, Murakami M, Nisitani S, Honjo T. Molecular mechanisms for B lymphocyte selection: induction and regulation of antigen-receptor-mediated apoptosis of mature B cells in normal mice and their defect in autoimmunity-prone mice. Philos Trans R Soc Lond B Biol Sci. 1994 Aug 30;345(1313):297-301. doi: 10.1098/rstb.1994.0109. PubMed PMID: 7531346. See more on PubMed
  • Durie FH, Fava RA, Foy TM, Aruffo A, Ledbetter JA, Noelle RJ. Prevention of collagen-induced arthritis with an antibody to gp39, the ligand for CD40. Science. 1993 Sep 3;261(5126):1328-30. doi: 10.1126/science.7689748. PubMed PMID: 7689748. See more on PubMed
  • Lane P, Brocker T, Hubele S, Padovan E, Lanzavecchia A, McConnell F. Soluble CD40 ligand can replace the normal T cell-derived CD40 ligand signal to B cells in T cell-dependent activation. J Exp Med. 1993 Apr 1;177(4):1209-13. doi: 10.1084/jem.177.4.1209. PubMed PMID: 7681469. PubMed CentralPMCID: PMC2190964. See more on PubMed
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