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Sex Hormone Binding Globulin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:SHBG, Sex steroid-binding protein, Testis-specific androgen-binding protein, Testosterone-estradiol-binding globulin, Testosterone-estrogen-binding globulin
  • Species:Human
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Cat. No. Size Price


RCD024R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

20 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

3.3–295 nmol/l

Limit of Detection

0.1 nmol/l

Spiking Recovery

100,00%

Dilutation Linearity

103,60%

Summary

Research topic

Reproduction, Steroid hormones

Summary

Sex hormone binding globulin (SHBG) is a glycoprotein composed of 373 amino acid residues and three carbohydrate side chains. SHBG has been known by many other names including Testosterone-estradiol Binding Globulin (TeBG), Sex steroid Binding Protein (sBP) and Sex Steroid Binding Globulin (SSBG). One of the main properties of SHBG is its high affinity for steroids, especially the C18, C19 and 17α-hydroxyl groups. The binding of steroids to SHBG is temperature and pH dependent. The three steroids that have a high avidity for SHBG are Dihydrotestosterone, Testosterone and Estradiol. Very small amounts of these steroids are free in biological fluid; the majority are bound to SHBG and albumin. These two fractions, that is, free and bound exist in a state of dynamic equilibrium. When the level of SHBG concentration changes, a remarkable change occurs in both albumin-bound hormone and also in the free fraction.
Throughout life SHBG increases until the eighties in both sexes. During the menstrual cycle SHBG does not seem to vary appreciably, however, according to some authors the concentration of SHBG is elevated in the luteal phase. During pregnancy the level of SHBG rises rapidly until about the 30th week of gestation.

References to Summary

References to Sex Hormone-Binding Globulin

  • Ahrentsen OD, Jensen HK, Johnsen SG. Sex-hormone-binding globulin deficiency. Lancet. 1982 Aug 14;2 (8294):377
  • Anderson DC. Sex-hormone-binding globulin. Clin Endocrinol (Oxf). 1974 Jan;3 (1):69-96
  • Belgorosky A, Rivarola MA. Progressive increase in non-sex-hormone-binding globulin-bound testosterone from infancy to late prepuberty in boys. J Clin Endocrinol Metab. 1987 Mar;64 (3):482-5
  • Cunningham GR, Tindall DJ, Lobl TJ, Campbell JA, Means AR. Steroid structural requirements for high affinity binding to human sex steroid binding protein (SBP). Steroids. 1981 Sep;38 (3):243-62
  • Dowsett M, Forbes KL, Rose GL, Mudge JE, Jeffcoate SL. A comparison of the effects of danazol and gestrinone on testosterone binding to sex hormone binding globulin in vitro and in vivo. Clin Endocrinol (Oxf). 1986 May;24 (5):555-63
  • Iqbal MJ, Johnson MW. Purification and characterization of human sex hormone binding globulin. J Steroid Biochem. 1979 May;10 (5):535-40
  • Ismail AA, Astley P, Cawood M, Short F, Wakelin K, Wheeler M. Testosterone assays: guidelines for the provision of a clinical biochemistry service. Ann Clin Biochem. 1986 Mar;23 ( Pt 2):135-45
  • Longcope C, Hui SL, Johnston CC Jr. Free estradiol, free testosterone, and sex hormone-binding globulin in perimenopausal women. J Clin Endocrinol Metab. 1987 Mar;64 (3):513-8
  • Moore JW, Bulbrook RD. The epidemiology and function of sex hormone-binding globulin. Oxf Rev Reprod Biol. 1988;10:180-236
  • Odlind V, Elamsson K, Englund DE, Victor A, Johansson ED. Effects of oestradiol on sex hormone binding globulin. Acta Endocrinol (Copenh). 1982 Oct;101 (2):248-53
  • Reed MJ, Cheng RW, Simmonds M, Richmond W, James VH. Dietary lipids: an additional regulator of plasma levels of sex hormone binding globulin. J Clin Endocrinol Metab. 1987 May;64 (5):1083-5
  • Rittmaster RS, Loriaux DL. Hirsutism. Ann Intern Med. 1987 Jan;106 (1):95-107
  • Rosner W. A simplified method for the quantitative determination of testosterone-estradiol-binding globulin activity in human plasma. J Clin Endocrinol Metab. 1972 Jun;34 (6):983-8
  • Rosner W, Smith RN. Isolation and characterization of the testosterone-estradiol-binding globulin from human plasma. Use of a novel affinity column. Biochemistry. 1975 Nov 4;14 (22):4813-20
  • Selby C. Sex hormone binding globulin: origin, function and clinical significance. Ann Clin Biochem. 1990 Nov;27 ( Pt 6):532-41
  • Vermeulen A, Ando S, Verdonck L. Prolactinomas, testosterone-binding globulin, and androgen metabolism. J Clin Endocrinol Metab. 1982 Feb;54 (2):409-12
  • von Schoultz B, Carlstrom K. On the regulation of sex-hormone-binding globulin--a challenge of an old dogma and outlines of an alternative mechanism. J Steroid Biochem. 1989 Feb;32 (2):327-34
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