Description
SophoMer™ F10 is a fully synthetic material that has been developed with an ambitious goal to replace the most commonly used nonspecific binding blocker – Bovine Serum Albumin
Source
Synthetic
Purity
˃ 95 %
Formulation
N-2-(hydroxypropyl)methacrylamide (HPMA)-based copolymer is lyophilized from destilled water.
Reconstitution
Add deionized water or appropriate buffer (pH 4.7 - 7.8) to prepare a working stock solution of 10 - 0.1 mg/mL.
Applications
ELISA, Biosensors, Immunoblotting
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store SophoMer™ F10 at 2 – 8 °C. Under these conditions, all components are stable until the expiration date (see label on the box).
Note
This product is intended for research use only.
Features
- SophoMer™ F10 is developed to support the world-leading developers of IVD immunology diagnosticsIs a more effective non-specific binding blocker.
- SophoMer™ F10 is a chemically defined material with minimal batch-to-batch variability.
- SophoMer™ F10 animal component-free material.
Summary
In vitro diagnostic methods face non-specific interactions increasing their background level and influencing the efficacy and reproducibility. Currently, the most important and employed blocker of non-specific interactions is bovine serum albumin (BSA), an animal product with some disadvantages like its batch-to-batch variability and contamination with RNases.
Amphiphilic water-soluble synthetic copolymers based on the highly biocompatible, non-immunogenic and nontoxic N-2-(hydroxypropyl)methacrylamide (HPMA)-based copolymers or poly(oxazoline)s is highly effective synthetic blockers of non-specific interactions and an effective BSA alternative.
The highest blocking capacity was observed for HPMA-based polymers containing two hydrophobic anchors taking advantage of the combination of two structurally different hydrophobic molecules.
