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sP-Selectin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Granule membrane protein 140, PADGEM, CD62P
  • Species:Human
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Cat. No. Size Price


RAF111R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.63–40.00 ng/ml

Limit of Detection

0.20 ng/ml

Intra-assay (Within-Run)

CV = 7.8%

Inter-assay (Run-to-Run)

CV = 5.4%

Dilutation Linearity

91,60%

Summary

Research topic

Cell adhesion proteins

Summary

P-selectin (CD62, GMP-140, PADGEM) belongs to the selectin family of adhesion molecules. P-selectin acts as a receptor that supports binding of leukocytes to activated platelets and endothelium. P-selectin-mediated adhesive interactions operate in conjunction with cell-cell interactions directed by related molecules and are likely to be important in both hemostatic and inflammatory processes.
P-selectin is located in membranes of a granules in unstimulated platelets and redistributed to the cell surface upon platelet activation. P-selectin is also present in endothelial cells in membranes of Weibel-Palade bodies and megakaryocytes. Surface appearance of P-selectin is very rapid, but transient declining to basal level within short time following stimulation.
P-selectin is a 140 kDa protein that is highly glycosylated. The cDNA-derived amino acid sequence predicts a molecule with a series of cysteine-rich domains. Like the other selectins P-selectin contains an N-terminal Ca2+ dependent lectin-like domain and an EGF-like motif which is followed by nine consensus repeats, a transmembrane domain, and a short cytoplasmic tail. The human gene for P-selectin is located on chromosome 1q21-24. P-selectin is a receptor for neutrophils and monocytes, recognizing oligosaccharide structures on the target cells. The physiologic role of P-selectin might be the mediation of initial leukocyte adhesion to activated endothelium during acute inflammation. It may work in concert with E-selectin to direct early, regionally specific adherence of neutrophils and monocytes at sites of acute inflammation. A soluble form of P-selectin found in serum and plasma has been described which might
represent a proteolytic fragment or more likely a soluble splice variant lacking the transmembrane domain.
Soluble P-selectin is a potentially important molecule to provide more detailed insight into pathological situations. Excessive accumulation of neutrophils on the endothelial surface accompanied by high exposure of P-selectin has been implicated in a number of inflammatory disorders, including adult respiratory distress syndrome, acute lung injury, ischemiareperfusion injury, Gram-negative septic shock, thrombotic diseases and rheumatoid arthritis. Malignant cells were shown to express receptors for P-selectin suggesting an important role for P-selectin in tumor formation and metastasis. Platelets have also been shown to promote tumor metastasis.

Summary References (18)

References to P-Selectin

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  • Finn A, Moat N, Rebuck N, Klein N, Strobel S, Elliott M. Changes in neutrophil CD11b/CD18 and L-selectin expression and release of interleukin 8 and elastase in paediatric cardiopulmonary bypass. Agents Actions. 1993;38 Spec No:C44-6
  • Foxall C, Watson SR, Dowbenko D, Fennie C, Lasky LA, Kiso M, Hasegawa A, Asa D, Brandley BK. The three members of the selectin receptor family recognize a common carbohydrate epitope, the sialyl Lewis(x) oligosaccharide. J Cell Biol. 1992 May;117 (4):895-902
  • Hogg N. Roll, roll, roll your leucocyte gently down the vein.... Immunol Today. 1992 Apr;13 (4):113-5
  • Jutila MA, Kishimoto TK, Finken M. Low-dose chymotrypsin treatment inhibits neutrophil migration into sites of inflammation in vivo: effects on Mac-1 and MEL-14 adhesion protein expression and function. Cell Immunol. 1991 Jan;132 (1):201-14
  • Kishimoto TK, Jutila MA, Berg EL, Butcher EC. Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors. Science. 1989 Sep 15;245 (4923):1238-41
  • Klein NJ, Levin M, Strobel S, Finn A. Degradation of glycosaminoglycans and fibronectin on endotoxin-stimulated endothelium by adherent neutrophils: relationship to CD11b/CD18 and L-selectin expression. J Infect Dis. 1993 Apr;167 (4):890-8
  • Lampeter ER, Kishimoto TK, Rothlein R, Mainolfi EA, Bertrams J, Kolb H, Martin S. Elevated levels of circulating adhesion molecules in IDDM patients and in subjects at risk for IDDM. Diabetes. 1992 Dec;41 (12):1668-71
  • Lasky LA. Lectin cell adhesion molecules (LEC-CAMs): a new family of cell adhesion proteins involved with inflammation. J Cell Biochem. 1991 Feb;45 (2):139-46
  • Mengelers HJ, Maikoe T, Hooibrink B, Kuypers TW, Kreukniet J, Lammers JW, Koenderman L. Down modulation of L-Selectin expression on eosinophils recovered from bronchoalveolar lavage fluid after allergen provocation. Clin Exp Allergy. 1993 Mar;23 (3):196-204
  • Moller P, Eichelmann A, Leithauser F, Mechtersheimer G, Otto HF. Venular endothelium binding molecules CD44 and LECAM-1 in normal and malignant B-cell populations. A comparative study. Virchows Arch A Pathol Anat Hi. 1992;421 (4):305-13
  • Schleiffenbaum B, Spertini O, Tedder TF. Soluble L-selectin is present in human plasma at high levels and retains functional activity. J Cell Biol. 1992 Oct;119 (1):229-38
  • Smith CW, Kishimoto TK, Abbassi O, Hughes B, Rothlein R, McIntire LV, Butcher E, Anderson DC. Chemotactic factors regulate lectin adhesion molecule 1 (LECAM-1)-dependent neutrophil adhesion to cytokine-stimulated endothelial cells in vitro. J Clin Invest. 1991 Feb;87 (2):609-18
  • Spertini O, Schleiffenbaum B, White-Owen C, Ruiz P Jr, Tedder TF. ELISA for quantitation of L-selectin shed from leukocytes in vivo. J Immunol Methods. 1992 Nov 25;156 (1):115-23
  • Stewart GJ. Neutrophils and deep venous thrombosis. Haemostasis. 1993 Mar;23 Suppl 1:127-40
  • Tozeren A, Ley K. How do selectins mediate leukocyte rolling in venules?. Biophys J. 1992 Sep;63 (3):700-9
  • Von Andrian UH, Hansell P, Chambers JD, Berger EM, Torres Filho I, Butcher EC, Arfors KE. L-selectin function is required for beta 2-integrin-mediated neutrophil adhesion at physiological shear rates in vivo. Am J Physiol. 1992 Oct;263 (4 Pt 2):H1034-44
  • Zimmerman GA, Prescott SM, McIntyre TM. Endothelial cell interactions with granulocytes: tethering and signaling molecules. Immunol Today. 1992 Mar;13 (3):93-100
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