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sPECAM-1 Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Platelet endothelial cell adhesion molecule, EndoCAM, CD31 antigen, Leukocyte surface protein
  • Species:Human
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Cat. No. Size Price


RAF109R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Urine, Plasma, Cell culture supernatant

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.48–30 ng/ml

Limit of Detection

0.06 ng/ml

Intra-assay (Within-Run)

CV = 1.7%

Inter-assay (Run-to-Run)

CV = 7.4%

Spiking Recovery

108,00%

Dilution Linearity

91,40%

Summary

Research topic

Cell adhesion proteins, Oncology

Summary

PECAM-1 (platelet endothelial cell adhesion molecule-1) also called CD31 and EndoCAM is a newly characterized adhesion molecule that belongs to the immunoglobulin superfamily [14]. PECAM-1 is a transmembrane glycoprotein with a molecular weight of approximately 130 kDa, depending on the degree of glycosylation.
PECAM-1 is constitutively expressed on all vascular cells and has provided a useful immunohistochemical marker of blood vessels, particularly in the setting of angiogenesis. It has also been found on platelets, monocytes, neutrophils and CD8+ T cells. Bone marrow stem cells and transformed cell lines of the myeloid and megakaryocytic lineage also express PECAM-1. Interestingly, PECAM-1 was also detected on human, mouse and rat solid tumor lines .
Recent studies suggest a role for PECAM-1 in the inflammatory process and leukocyte-endothelial interaction. The process of leukocyte emigration to the site of inflammation can be dissected into three successive stages: rolling, mediated by the selectins; tight adhesion mediated by ICAMs and their counter-receptors, the integrins; and transmigration of leukocytes through intercellular junctions of vascular endothelial cells which requires PECAM-1. PECAM-1 appears to be able to interact both with itself (homophilic binding) and with other "non-PECAM-1" molecules (heterophilic binding).
PECAM-1 is an early and sensitive marker for tumor-induced angiogenesis. Several data have suggested that PECAM-1 may be involved in the process of angiogenesis in a developing vertebrate embryo as well as during metastases formation.
Besides the membrane-bound form of PECAM-1 a soluble form of the molecule exists, which is 5-10 kDa smaller than cell-associated PECAM-1, and contains the cytoplasmic tail. This form of soluble PECAM-1 is encoded by an alternatively spliced mRNA from which the exon containing the transmembrane domain has been removed. Soluble PECAM-1 was detected in normal human plasma. Antibodies against PECAM-1 have been shown to exhibit a high degree of sensitivity and specificity for endothelial cells in normal, inflammatory and neoplastic tissue. PECAM-1 is a sensitive and specific marker for detection of melanoma-associated angiogenesis. Thus, PECAM-1 may be a useful marker for early detection of tumor metastasis. Compared to normal gut, there is a significant increase of PECAM-1-positive vessels in the mucosa of uninvolved ulcerative colitis. In gingivitis and periodontitis lesion the expression of PECAM-1 on mononuclear infiltrates increases significantly with increasing size of infiltrate.

Product References (1)

References

  • Polus A, Zapala B, Razny U, Gielicz A, Kiec-Wilk B, Malczewska-Malec M, Sanak M, Childs CE, Calder PC, Dembinska-Kiec A. Omega-3 fatty acid supplementationinfluences the whole blood transcriptome in women with obesity, associated withpro-resolving lipid mediator production. Biochim Biophys Acta. 2016Nov;1861(11):1746-1755. doi: 10.1016/j.bbalip.2016.08.005. Epub 2016 Aug 12.PubMed PMID: 27531277. See more on PubMed
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