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Manufactured by BioVendor

Sperm-Antibody Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:Anti-Spermatozoa Antibody, Anti-Sperm Antibody
  • Species:Human
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Cat. No. Size Price


RIS0017R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

31–250 U/ml

Intra-assay (Within-Run)

6.88% (5.90 – 7.81 %)

Inter-assay (Run-to-Run)

6.45% (4.84 – 7.52 %)

Summary

Research topic

Reproduction

Summary

Antibodies directed against spermatozoa antigens may cause infertility in women or men. The application of the Anti-Spermatozoa Antibody ELISA from Biovendor is recommended for the diagnosis of immunologically caused disorders of fertility. Unwanted childlessness is a growing problem with which up to 20% of all couples in the reproductive age are confronted temporarily or long-term. In 20% of these cases the presence of anti-spermatozoa antibodies in the male or the female patient is detectable (Lahteenmaki A et al: Hum Reprod (1995) 10, 2824-28; Nagy ZP et al: Hum Reprod (1995) 10, 1775-80).
The definition of infertility according to the WHO (WHO Laboratory Manual for the Examination of Human Semen and Semen Cervical-Mucus Interaction, 1999) is the absence of a conception within 12 months of unprotected intercourse. The main cause of an immunological fertility disorder is the formation of antibodies directed against
spermatozoa antigens.
Anti-spermatozoa antibodies exert heterogeneous effects on the ability of spermatozoa
to fertilize. The inhibiting effect of anti-spermatozoa antibodies on the motility of spermatozoa
by binding to their surface and by agglutinating processes is well-known (Zouari R et al: Fertil
Steril (1993) 59, 606-12).
The penetration of the spermatozoa into the cervical mucus is impaired by the presence
of anti-spermatozoa antibodies in the seminal plasma and/or in the cervical mucus (Eggert-
Kruse W et al: Hum Reprod (1993) 8, 1025-31). Anti-spermatozoa antibodies negatively
influence the capacitation and the acrosome reaction of spermatozoa and thereby impede
the interaction of the spermatozoa with the oocyte (Francavilla F et al: Front Biosci (1999):
1;4:9-25; Bohring C et al.: Hum Reprod (2001) 7:113-8).
The interaction of the spermatozoon with the oocyte and the subsequent binding to and
penetration of the zona pellucida may be inhibited by anti-spermatozoa antibodies.
The following fusion of the oocyte and a spermatozoon may also be impaired by the presence
of anti-spermatozoa antibodies (Mazumdar S et al.: Fertil Steril (1998) 70, 799-810; Kutteh
WH: Hum Reprod, (1999) 14, 2426-9).
According to Crosignani et al. (Crosignani et al.: PG et al.: Hum Reprod (1998) 13, 2025-32)
the rate of pregnancies in couples with anti-spermatozoa antibodies on the part of the man
or the woman are 38% lower compared to the control groups. Furthermore an influence
on the implantation and on the early embryological development could be confirmed.
An association of anti-spermatozoa antibodies and miscarriages is discussed.
The frequency of anti-spermatozoa antibodies in infertile couples amounts to 20%
(Lahteenmaki A et al.: Hum Reprod (1995) 10, 2824-28; Nagy ZP et al.: Hum Reprod (1995)
10, 1775-80).
Anti-spermatozoa antibodies may occur dissolved in the ejaculate or bound to the surface
of spermatozoa. Anti-spermatozoa antibodies may be found in men and in women (Clarke GN
et al.: Am J Reprod Immunol Microbiol (1985) 7, 143-7). In women anti-spermatozoa
antibodies may be found in cervical mucus, oviduct liquid and follicular liquid. Men having more
than 50% of their spermatozoa coated with anti-spermatozoa antibodies show a conspicuously
reduced rate of fertility (Abshagen K et al.: Fertil Steril (1998) 70, 355-6).

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