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Manufactured by BioVendor

Stem Cell Factor Human

  • Regulatory status:RUO
  • Type:Recombinant protein
  • Source:E. coli
  • Species:Human
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Cat. No. Size Price


RP1762550010 0.01 mg
PubMed Product Details
Technical Data

Type

Recombinant protein

Description

Recombinant Human SCF produced in E.Coli is a single, non-glycosylated polypeptide chain containing 164 amino acids and having a molecular mass of 18.4 kDa. The SCF is purified by proprietary chromatographic techniques.

Amino Acid Sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Gly-Ile-Cys.

Source

E. coli

Purity

Greater than 98.0% as determined by: - (a) Analysis by RP-HPLC. - (b) Anion-exchange FPLC. - © Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel.

Biological Activity

BioVendor´s re­combinant human SCF is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of Human TF-1 cells is < 2 ng/ml, corresponding to a Specific Activity of 5×105 IU/mg.

Endotoxin

Less than 0.1 ng/Cg (IEU/Cg) of rHuSCF.

Formulation

Lyophilized from a concentrated (1mg/ml) solution in water containing 10mM Acetic acid.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Lyophilized rHuSCF although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution rHuSCF should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Physical Appearance

Sterile filtered white lyophilized (freeze-dried) powder.

Summary

Research topic

Cytokines and chemokines and related molecules

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