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sTfR Human ELISA (soluble Transferrin Receptor)

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:TfR
  • Species:Human
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Cat. No. Size Price


RD194011100 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.05–2 µg/ml

Limit of Detection

2 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.8%

Inter-assay (Run-to-Run)

n = 4; CV = 6.3%

Spiking Recovery

100,20%

Dilutation Linearity

94,00%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • goat Yes
  • mouse Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • monkey Yes
  • pig Yes
  • human Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 3 hours
  • The kit measures total soluble transferrin receptor in serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Quality Controls are human serum based. No animal sera are used
  • Standard is natural human blood isolated sTfR based
  • Components of the kit are provided ready to use or concentrated

Research topic

Iron metabolism, Oncology

Summary

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

References to Product

References

  • Boshuizen M, Binnekade JM, Nota B, et al. Iron metabolism in critically ill patients developing anemia of inflammation: a case control study. Ann Intensive Care. 2018;8(1):56. Published 2018 May 2. doi:10.1186/s13613-018-0407-5
  • Brzóska K, Bartłomiejczyk T, Sochanowicz B, et al. Carcinogenesis-related changes in iron metabolism in chronic obstructive pulmonary disease subjects with lung cancer. Oncol Lett. 2018;16(5):6831-6837.
  • Dierschke K, Isaxon C, Andersson UB, Assarsson E, Axmon A, Stockfelt L, Gudmundsson A, Jönsson BA, Kåredal M, Löndahl J, Pagels J, Wierzbicka A, Bohgard M, Nielsen J. Acute respiratory effects and biomarkers of inflammation due to welding-derived nanoparticle aggregates. Int Arch Occup Environ Health. 2017 Mar 3. doi: 10.1007/s00420-017-1209-z
  • El-Gendy Fady, El-Hawy MA, Rizk MS, El-Hefnawy SM, Mahmoud MZ. Value of Soluble Transferrin Receptors and sTfR/log Ferritin in the Diagnosis of Iron Deficiency Accompanied by Acute Infection. 25 May 2017. 10.1007/s12288-017-0836-6.
  • Fernandez-Real JM, Izquierdo M, Moreno-Navarrete JM, Gorostiaga E, Ortega F, Martinez C, Idoate F, Ricart W, Ibanez J. Circulating soluble transferrin receptor concentration decreases after exercise-induced improvement of insulin sensitivity in obese individuals. Int J Obes (Lond). 2009 Jul;33 (7):768-74
  • Fernandez-Real JM, Moreno JM, Lopez-Bermejo A, Chico B, Vendrell J, Ricart W. Circulating soluble transferrin receptor according to glucose tolerance status and insulin sensitivity. Diabetes Care . Mar;30(3):604-8 (2007)
  • Jafari SM, Heidari G, Nabipour I, Amirinejad R, Assadi M, Bargahi A, Akbarzadeh S, Tahmasebi R, Sanjdideh Z. Serum retinol levels are positively correlated with hemoglobin concentrations, independent of iron homeostasis: a population-based study. Nutr Res. 2013 Apr;33 (4):279-85
  • Khambalia A, O'Connor DL, Zlotkin S. Periconceptional iron and folate status is inadequate among married, nulliparous women in rural Bangladesh. J Nutr. 2009 Jun;139 (6):1179-84
  • Menon KC, Ferguson EL, Thomson CD, Gray AR, Zodpey S, Saraf A, Das PK, Pandav CS, Skeaff SA. Iron status of pregnant Indian women from an area of active iron supplementation. Nutrition. 2014 Mar;30 (3):291-6
  • Mojiminiyi OA, Marouf R, Abdella NA. Body iron stores in relation to the metabolic syndrome, glycemic control and complications in female patients with type 2 diabetes. Nutr Metab Cardiovasc Dis. 2008 Oct;18 (8):559-66
  • Rybka J, Kedziora-Kornatowska K, Banas-Lezanska P, Majsterek I, Carvalho LA, Cattaneo A, Anacker C, Kedziora J. Interplay between the pro-oxidant and antioxidant systems and proinflammatory cytokine levels, in relation to iron metabolism and the erythron in depression. Free Radic Biol Med. 2013 Oct;63:187-94
  • Sharma JB, Bumma SD,Saxena R,Kumar S,Roy KK,Singh N,Vanamail P. Cross sectional, comparative study of serum erythropoietin, transferrin receptor, ferritin levels and other hematological indices in normal pregnancies and iron deficiency anemia during pregnancy. EJOG. May 2016;:5
  • Shinzato T, Abe K, Furusu A, Harada T, Shinzato K, Miyazaki M, Kohno S. Serum pro-hepcidin level and iron homeostasis in Japanese dialysis patients with erythropoietin (EPO)-resistant anemia. Med Sci Monit. 2008 Sep;14 (9):CR431-7
  • Skarpańska-Stejnborn A, Basta P, Trzeciak J, Michalska A, Kafkas ME, Woitas-Ślubowska D. Effects of cranberry (Vaccinum macrocarpon) supplementation on iron status and inflammatory markers in rowers. J Int Soc Sports Nutr. 2017 Feb 28;14:7. doi: 10.1186/s12970-017-0165-z
  • Stefanova KI, Delcheva GT, Maneva AI, Batalov AZ, Geneva-Popova MG, Karalilova RV, Simitchiev KK. Pathobiochemical Mechanisms Relating Iron Homeostasis to Parameters of Inflammatory Activity and Autoimmune Disorders in Rheumatoid Arthritis. Folia Med (Plovdiv). 2016 Dec 1;58(4):257-263. doi: 10.1515/folmed-2016-0040
  • Tiwari AK, Bhardwaj G, Arora D, Aggarwal G, Pabbi S, Dara RC, Sachdev R, Raizada A, Sethi M. Applying newer parameter Ret-He (reticulocyte haemoglobin equivalent) to assess latent iron deficiency (LID) in blood donors-study at a tertiary care hospital in India. Vox Sang. 2018 Aug 12. doi: 10.1111/vox.12700.
  • Fukao W, Hasuike Y, Yamakawa T, Toyoda K, Aichi M, Masachika S, Kantou M, Takahishi SI, Iwasaki T, Yahiro M, Nanami M, Nagasawa Y, Kuragano T, Nakanishi T. Oral Versus Intravenous Iron Supplementation for the Treatment of Iron Deficiency Anemia in Patients on Maintenance Hemodialysis-Effect on Fibroblast Growth Factor-23 Metabolism. J Ren Nutr. 2018 Jul;28(4):270-277. doi: 10.1053/j.jrn.2017.12.009. Epub 2018 Apr 24.
  • Büyükkaragöz B, Akgun NA, Bulus AD, Durmus Aydogdu S, Bal C. Can soluble transferrin receptor be used in diagnosing iron deficiency anemia and assessing iron response in infants with moderate acute malnutrition? Arch Argent Pediatr. 2017 Apr 1;115(2):125-132. doi: 10.5546/aap.2017.eng.125.
References to Summary

References to Transferrin receptor

  • Raya G. et al.: Soluble transferrin receptor (sTfR): biological variations and reference limits. Clinical Chemistry and Laboratory Medicine, 39, 1162–1168 (2001)
  • Cotton F. et al.: Measurement of soluble transferrin receptor by immunoturbidimety and immunonefelometry. Clinical Biochemistry, 33, 263–267 (2000)
  • Cook J. D.: The measurement of serum transferrin receptor. The American Journal of the Medical Sciences 318, 269–276 (1999)
  • Olivares M. et al.: Usefulness of serum transferrin receptor and serum ferritin in diagnosis of iron deficiency in infancy. American Journal of Clinical Nutrition 72, 1191–1195 (2000)
  • Suominen P. et al.: Single values of serum transferrin receptor and transferrin receptor ferritin index can be used to detect true and functional iron deficiency in rheumatoid arthritis patients with anemia. Arthritis & Rheumatism 43, 1016–1020 (2000)
  • De Block C. E. M. et al.: Soluble transferrin receptor level. A new marker for iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1diabetes. Diabetes Care 23, 1384–1388 (2000)
  • Kolbe-Busch S. et al. Determination of the soluble transferrin receptor in Serum: Evaluation of two enzyme immunoassays and a particle-enhanced immunonephelometric assay. Clinical Laborators 45, 295–304 (1999)
  • Hikawa A. et al.: Soluble transferrin receptor-transferrin complex in serum: measurement by latex agglutination nephelometric asssay. Clinica Chimica Acta 254, 159–172 (1996)
  • Flowers C. H. et al.: The clinical measurement of serum transferrin receptor. Journal of Laboratory and Clinical Medicine 114, 368–377 (1989)
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