sTfR Human ELISA (soluble Transferrin Receptor)

Cat. No.	Size	Price
RD194011100	96 wells (1 kit)	$488

Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.05–2 µg/ml

Limit of Detection

2 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.8%

Inter-assay (Run-to-Run)

n = 4; CV = 6.3%

Spiking Recovery

100,20%

Dilution Linearity

94,00%

Crossreactivity

bovine Non-detectable
cat Non-detectable
dog Non-detectable
hamster Non-detectable
horse Non-detectable
goat Yes
mouse Non-detectable
rabbit Non-detectable
rat Non-detectable
sheep Non-detectable
chicken Not tested
monkey Yes
pig Yes
human Yes

Note

The kits are CE-IVD certified and intended for professional use.

Summary

Features

European Union: for in vitro diagnostic use
Rest of the world: for research use only!
The total assay time is less than 3 hours
The kit measures total soluble transferrin receptor in serum and plasma (EDTA, citrate, heparin)
Assay format is 96 wells
Quality Controls are human serum based. No animal sera are used
Standard is natural human blood isolated sTfR based
Components of the kit are provided ready to use or concentrated

Research topic

Iron metabolism, Oncology

Summary

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

Product References (23)

References

Summary References (9)

References to Transferrin receptor

Raya G. et al.: Soluble transferrin receptor (sTfR): biological variations and reference limits. Clinical Chemistry and Laboratory Medicine, 39, 1162–1168 (2001)
Cotton F. et al.: Measurement of soluble transferrin receptor by immunoturbidimety and immunonefelometry. Clinical Biochemistry, 33, 263–267 (2000)
Cook J. D.: The measurement of serum transferrin receptor. The American Journal of the Medical Sciences 318, 269–276 (1999)
Olivares M. et al.: Usefulness of serum transferrin receptor and serum ferritin in diagnosis of iron deficiency in infancy. American Journal of Clinical Nutrition 72, 1191–1195 (2000)
Suominen P. et al.: Single values of serum transferrin receptor and transferrin receptor ferritin index can be used to detect true and functional iron deficiency in rheumatoid arthritis patients with anemia. Arthritis & Rheumatism 43, 1016–1020 (2000)
De Block C. E. M. et al.: Soluble transferrin receptor level. A new marker for iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1diabetes. Diabetes Care 23, 1384–1388 (2000)
Kolbe-Busch S. et al. Determination of the soluble transferrin receptor in Serum: Evaluation of two enzyme immunoassays and a particle-enhanced immunonephelometric assay. Clinical Laborators 45, 295–304 (1999)
Hikawa A. et al.: Soluble transferrin receptor-transferrin complex in serum: measurement by latex agglutination nephelometric asssay. Clinica Chimica Acta 254, 159–172 (1996)
Flowers C. H. et al.: The clinical measurement of serum transferrin receptor. Journal of Laboratory and Clinical Medicine 114, 368–377 (1989)

By Research Topic

Iron metabolism
Oncology

By Molecule

Transferrin receptor

By Technical Data

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Product Brochure

CE IVD Assays