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sTfR Human, Mouse Monoclonal Antibody, Clone: TFR-01

  • Regulatory status:RUO
  • Type:Monoclonal Antibody
  • Other names:TfR
  • Species:Human
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Cat. No. Size Price


RD182011110-01 0.1 mg
PubMed Product Details
Technical Data

Type

Monoclonal Antibody

Applications

Western blotting, ELISA

Source of Antigen

Human placenta

Hosts

Mouse

Isotype

IgG1

Clone

TFR-01

Preparation

The antibody is a mouse monoclonal antibody against Human sTfR.

Purification Method

Affinity chromatography on a column with immobilized protein G.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.1 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

SDS PAGE – to determine purity of the antibody.

Note

This product is for research use only.

Summary

Research topic

Iron metabolism, Oncology

Summary

The transferrin receptor (TfR) is the gateway for transferrin-bound-iron entering all body cells. TfR is abundant on the surface of many newly formed cells, but the erythroid marrow cells account for 70 to 80 % of the total body TfR content. The soluble (or serum) transferrin receptor (sTfR) is a circulating truncated form of the membrane receptor protein; it is an 85 kDa glycoprotein forming in serum a 320 kDa complex with diferric transferrin. The serum sTfR concentration reflects the total body mass of cellular transferrin receptor. Anaemias associated with enhanced erythropoiesis and iron deficiency result in an elevation in the sTfR values. Elevation of the soluble transferrin receptor may be also caused by haemolytic anaemia, polycythaemia and thalassemia while aplastic anaemia and chronic renal failure may result in its decrease. The most important clinical use of the sTfR determination is in the differential diagnosis between iron deficiency anaemia and the anaemia of chronic disease.

References to Summary

References to Transferrin receptor

  • Raya G. et al.: Soluble transferrin receptor (sTfR): biological variations and reference limits. Clinical Chemistry and Laboratory Medicine, 39, 1162–1168 (2001)
  • Cotton F. et al.: Measurement of soluble transferrin receptor by immunoturbidimety and immunonefelometry. Clinical Biochemistry, 33, 263–267 (2000)
  • Cook J. D.: The measurement of serum transferrin receptor. The American Journal of the Medical Sciences 318, 269–276 (1999)
  • Olivares M. et al.: Usefulness of serum transferrin receptor and serum ferritin in diagnosis of iron deficiency in infancy. American Journal of Clinical Nutrition 72, 1191–1195 (2000)
  • Suominen P. et al.: Single values of serum transferrin receptor and transferrin receptor ferritin index can be used to detect true and functional iron deficiency in rheumatoid arthritis patients with anemia. Arthritis & Rheumatism 43, 1016–1020 (2000)
  • De Block C. E. M. et al.: Soluble transferrin receptor level. A new marker for iron deficiency anemia, a common manifestation of gastric autoimmunity in type 1diabetes. Diabetes Care 23, 1384–1388 (2000)
  • Kolbe-Busch S. et al. Determination of the soluble transferrin receptor in Serum: Evaluation of two enzyme immunoassays and a particle-enhanced immunonephelometric assay. Clinical Laborators 45, 295–304 (1999)
  • Hikawa A. et al.: Soluble transferrin receptor-transferrin complex in serum: measurement by latex agglutination nephelometric asssay. Clinica Chimica Acta 254, 159–172 (1996)
  • Flowers C. H. et al.: The clinical measurement of serum transferrin receptor. Journal of Laboratory and Clinical Medicine 114, 368–377 (1989)
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