Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

sTNF-R I Monkey ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:TNFR1, Tumor necrosis factor receptor superfamily member 1A, p60, p55, CD120a antigen
  • Species:Monkey
Please select your region to see available products and prices.
Cat. No. Size Price


RAF116R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Baboon serum

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.09–6 U/ml

Limit of Detection

0.05 U/ml

Intra-assay (Within-Run)

CV = 2.0%

Inter-assay (Run-to-Run)

CV = 9.0%

Spiking Recovery

93,00%

Dilutation Linearity

105,00%

Summary

Research topic

Cytokines and chemokines and related molecules, Animal studies

Summary

Tumor Necrosis Factor (TNF) was originally discovered in sera of animals and was found to cause hemorrhagic necrosis of some transplantable mouse and human tumors and to exhibit primarily cytotoxic activities against tumor but not normal cells in vitro. The TNF family consists of two proteins designated TNFα, also called cachectin, and TNFβ, also called lymphotoxin, which are pleiotropic cytokines that can mediate a wide variety of biological effects.

References to Summary

References to TNF-R (60 kDa)

  • Adolf GR, Apfler I. A monoclonal antibody-based enzyme immunoassay for quantitation of human tumor necrosis factor binding protein I, a soluble fragment of the 60 kDa TNF receptor, in biological fluids. J Immunol Methods. 1991 Sep 20;143 (1):127-36
  • Aggarwal BB, Eessalu TE, Hass PE. Characterization of receptors for human tumour necrosis factor and their regulation by gamma-interferon. Nature. 1985 Dec 19-1986 Jan 1;318 (6047):665-7
  • Baglioni C, McCandless S, Tavernier J, Fiers W. Binding of human tumor necrosis factor to high affinity receptors on HeLa and lymphoblastoid cells sensitive to growth inhibition. J Biol Chem. 1985 Nov 5;260 (25):13395-7
  • Beutler B, Cerami A. Cachectin: more than a tumor necrosis factor. N Engl J Med. 1987 Feb 12;316 (7):379-85
  • Beutler B, Mahoney J, Le Trang N, Pekala P, Cerami A. Purification of cachectin, a lipoprotein lipase-suppressing hormone secreted by endotoxin-induced RAW 264.7 cells. J Exp Med. 1985 May 1;161 (5):984-95
  • Brockhaus M, Schoenfeld HJ, Schlaeger EJ, Hunziker W, Lesslauer W, Loetscher H. Identification of two types of tumor necrosis factor receptors on human cell lines by monoclonal antibodies. Proc Natl Acad Sci U S A. 1990 Apr;87 (8):3127-31
  • Carswell EA, Old LJ, Kassel RL, Green S, Fiore N, Williamson B. An endotoxin-induced serum factor that causes necrosis of tumors. Proc Natl Acad Sci U S A. 1975 Sep;72 (9):3666-70
  • Creasey AA, Yamamoto R, Vitt CR. A high molecular weight component of the human tumor necrosis factor receptor is associated with cytotoxicity. Proc Natl Acad Sci U S A. 1987 May;84 (10):3293-7
  • Himmler A, Maurer-Fogy I, Kronke M, Scheurich P, Pfizenmaier K, Lantz M, Olsson I, Hauptmann R, Stratowa C, Adolf GR. Molecular cloning and expression of human and rat tumor necrosis factor receptor chain (p60) and its soluble derivative, tumor necrosis factor-binding protein. DNA Cell Biol. 1990 Dec;9 (10):705-15
  • Hohmann HP, Remy R, Brockhaus M, van Loon AP. Two different cell types have different major receptors for human tumor necrosis factor (TNF alpha). J Biol Chem. 1989 Sep 5;264 (25):14927-34
  • Kull FC Jr, Jacobs S, Cuatrecasas P. Cellular receptor for 125I-labeled tumor necrosis factor: specific binding, affinity labeling, and relationship to sensitivity. Proc Natl Acad Sci U S A. 1985 Sep;82 (17):5756-60
  • Loetscher H, Pan YC, Lahm HW, Gentz R, Brockhaus M, Tabuchi H, Lesslauer W. Molecular cloning and expression of the human 55 kd tumor necrosis factor receptor. Cell. 1990 Apr 20;61 (2):351-9
  • Munker R, DiPersio J, Koeffler HP. Tumor necrosis factor: receptors on hematopoietic cells. Blood. 1987 Dec;70 (6):1730-4
  • Old LJ. Tumor necrosis factor (TNF). Science. 1985 Nov 8;230 (4726):630-2
  • Olsson I, Lantz M, Nilsson E, Peetre C, Thysell H, Grubb A, Adolf G. Isolation and characterization of a tumor necrosis factor binding protein from urine. Eur J Haematol. 1989 Mar;42 (3):270-5
  • Paul NL, Ruddle NH. Lymphotoxin. Annu Rev Immunol. 1988;6:407-38
  • Rubin BY, Anderson SL, Sullivan SA, Williamson BD, Carswell EA, Old LJ. High affinity binding of 125I-labeled human tumor necrosis factor (LuKII) to specific cell surface receptors. J Exp Med. 1985 Sep 1;162 (3):1099-104
  • Schall TJ, Lewis M, Koller KJ, Lee A, Rice GC, Wong GH, Gatanaga T, Granger GA, Lentz R, Raab H, et al. Molecular cloning and expression of a receptor for human tumor necrosis factor. Cell. 1990 Apr 20;61 (2):361-70
  • Scheurich P, Thoma B, Ucer U, Pfizenmaier K. Immunoregulatory activity of recombinant human tumor necrosis factor (TNF)-alpha: induction of TNF receptors on human T cells and TNF-alpha-mediated enhancement of T cell responses. J Immunol. 1987 Mar 15;138 (6):1786-90
  • Scheurich P, Ucer U, Kronke M, Pfizenmaier K. Quantification and characterization of high-affinity membrane receptors for tumor necrosis factor on human leukemic cell lines. Int J Cancer. 1986 Jul 15;38 (1):127-33
  • Smith CA, Davis T, Anderson D, Solam L, Beckmann MP, Jerzy R, Dower SK,
Related Products Documents