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  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Vascular endothelial growth factor receptor 1, Vascular permeability factor receptor, Tyrosine-protein kinase receptor FLT, Tyrosine-protein kinase FRT, Flt1, Flt-1
  • Species:Human
Cat. No. Size Price

RAF121R 96 wells (1 kit) $721,83
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


The Human sVEGF-R1 ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of Human sVEGF-R1.


Serum, Plasma-EDTA, Plasma-Citrate, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.16–10 ng/ml

Limit of Detection

0.03 ng/ml

Intra-assay (Within-Run)

CV = 5.5%

Inter-assay (Run-to-Run)

CV = 5.1%

Dilution Linearity

serum 128%, EDTA plasma 125%, citrate plasma 102%, cell culture supernatant 116%


The assay detects both natural and recombinant Human sVEGF-R1. The cross reactivity and interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a Human sVEGF-R1 positive sample. Interference was detected for VEGF-A at concentrations > 0.15 ng/ml and for PLGF-1 at concentrations > 2.5 ng/ml.There was no cross reactivity or interference detected for KDR, PDGFAA, PDGF-BB, VEGF-B, VEGF-C, VEGF-D, HGF, EGF.



  • RUO
  • calibration range 0.16-10 ng/ml
  • limit of detection 0.03%
  • intra-assay CV = 5.5%
  • inter-assay CV = 5.1%

Research topic

Cytokines and chemokines and related molecules


Soluble VEGF-R1 (sFLT-1) is a naturally occurring endogenous form of the VEGF-R1 and was originally found in the supernatant of Human vascular endothelial cells. It is generated by differential splicing of the flt-1 gene. In vitro sVEGF-R1 is used to inhibit VEGF-A mediated signals in endothelial cells and in vivo it can be used to block physiological angiogenesis in several organs, e.g. in the ovary or in bones. Tumor cells transfected with the flt-1 gene are growth restricted in vivo because of the limitation in developing tumor blood vessels via VEGF-A signalling. Very recent studies have shown that this molecule is present endogenously at ng/ml concentrations in biologicals fluids of normal Human subjects or in the conditioned media of FLT-1 positive cell types.

Summary References (2)

References to VEGF receptor

  • Imbert A, Rosnet O, Marchetto S, Ollendorff V, Birnbaum D, Pebusque MJ. Characterization of a yeast artificial chromosome from human chromosome band 13q12 containing the FLT1 and FLT3 receptor-type tyrosine kinase genes. Cytogenet Cell Genet. 1994;67 (3):175-7
  • Niida S, Kondo T, Hiratsuka S, Hayashi S, Amizuka N, Noda T, Ikeda K, Shibuya M. VEGF receptor 1 signaling is essential for osteoclast development and bone marrow formation in colony-stimulating factor 1-deficient mice. Proc Natl Acad Sci U S A. 2005 Sep 27;102 (39):14016-21
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