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Technical Specification

Microscope configuration transmission inverted microscope
Microscopy techniques holography (quantitative phase imaging), epifluorescence, simulated DIC, brightfield
Objectives magnification 4× to 60×
Objective turret 6-position, motorized exchange
Light source halogen lamp
Operating wavelength 650 nm
Sample stage motorized, 130 mm × 90 mm travel range
Focusing motorized objective turret, 8 mm travel range
Piezo-focusing optional, multiple travel ranges available
Lateral resolution 3.3 μm with 4× NA 0.1 objective 0.57 μm with 60× NA 1.4 objective
Field of view objective dependent, up to 950 μm × 950 μm with 4× objective
Acquisition framerate 5.5 fps at full frame (option: higher framerates possible)
Reconstructed phase image size 600 px × 600 px
Illumination power at sample plane down to 0.2 μW/cm2
Phase detection sensitivity down to 0.0035 rad (0.7 nm at Δn = 0.5) Δn - difference between refractive indexes of sample and surrounding media
Power 230 V/50 Hz (120 V/60 Hz optional), 2300 VA
Dimensions (W × L × H) 1100 mm × 950 mm × 1620 mm microscope with incubator 2515 mm × 974 mm × 1620 mm total with operator table
Weight 350 kg (including microscope table, epi-fluorescence attachment and microscope incubator)

 

Field and aperture diaphragms
Side port available for fluorescence module or other additional techniques
Microscope table with anti-vibration suspension
Control panel with multifunctional touchscreen, sample stage joystick and rotary knobs
Microscope incubator with computer temperature setting and temperature data logging
Incubation chamber for precise and long-term control of temperature, humidity and CO2 concentrations.

 

microRNA IN A NEW LIGHT!

microRNA IN A NEW LIGHT!

MicroRNAs (miRNAs) are new and promising potential biomarkers for diagnosis and prognosis of many diseases, which opens up a new field of goals and challenges for the BioVendor R&D team. Development of miRNA diagnostic assays is just the first step; our mission is to provide validated kits to measure individual miRNAs or diagnostically relevant miRNA “groups” supported with tools for standardized miRNA isolation, sample collection, and pretreatment.

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MicroRNAs (miRNAs) are small, non-coding RNA molecules typically containing ~22 nucleotides found in human, animals, plants and some viruses which are playing an important regulatory role in the gene translation via silencing or degradation of target mRNAs. During the RNA silencing, miRNA functions as a complementary guide which binds with its target mRNAs, followed by action of AGO proteins family which works as effectors by recruiting factors that induce translational repression, mRNA deadenylation and mRNA decay. (Minju Ha, Nature Reviews, 2014)

MicroRNAs (miRNAs) are involved in virtually all physiologic processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation. The biogenesis and function of miRNAs are tightly regulated, and their dysregulation is often associated with pathology of many diseases such as cancer, heart disease or neurological disorders. Recently, the miRNA has been proposed as powerful biomarker for prediction of the diseases, treatment response or prediction of progression.

 

ID3EAL Biofluid miRNA Panel

ID3EAL Biofluid miRNA Panel

MiRXES ID3EAL Biofluid miRNA knowledge panel was compared to equivalent products from two leading vendors using a common pool of human serum RNA. For each product, RNA extracted from 200 μL of pooled human serum was used at the start of respective workflows. Detection cut-offs were determined based on the respective manufacturers’ instructions (CT ≤ 33 for ID3EAL Biofluid Panel). Of the 3 biofluid miRNA products, the ID3EAL Biofluid Panel detected the highest number of microRNA.

11029

Separately, the ID3EAL Biofluid Panel was used to illustrate distinct miRNA profiles in normal and gastric cancer patient serum.
11030
Format of Panel

 

11051 

Biofluid miRNA knowledge panel

 

CAT. NO. Name DESCRIPTION SAMPLES
1105151 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
2 x 96-well PCR plates
2
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105251 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (2) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
4 x 96-well PCR plates
2
1105252 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
6
1105253 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
12
1105254 ID3EAL miRNA Knowledge Panel 192 Targets, 96-well (24) 4 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
24

 

For Purchase Calculation please download Knowledge Panel calculator here:

Knowledge Panel calculator

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Biofluid miRNA Panel Analysis Template 

 

ID3EAL Cancer miRNA Panel

ID3EAL Cancer miRNA Panel

Four distinct cell types (HDFa, BE2C, HCT116 and U251) were profiled with the ID3EAL Cancer miRNA Panel. One hundred nanograms of RNA from each sample was used for reverse transcription. About 300 miRNAs were detected for each cell line, with 244 common miRNAs detected with CT ≤ 33 across all 4 cell lines.

11056
Number of miRNAs Detected                                                  CT number of miRNAs Detected

Inter-plate calibrator normalization and biological normalization by global mean or reference miRNAs were performed prior to inter-sample miRNA ΔCT calculation. MiRNA expression comparison in HFDa and U251 cells are visualized through a scatter plot. Data points that fall outside of ±1 cycles regression lines indicate miRNAs differentially expressed in the both cell lines.


11049

Format of Panel

11051

 Cancer miRNA knowledge panel 

CAT. NO. Name DESCRIPTION SAMPLES
1105151 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (2) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
2 x 96-well PCR plates
2
1105152 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (6) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
6 x 96-well PCR plates
6
1105153 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (12) 1 x 24 μl of ID3EAL miRNA RT Primers 96-plex
12 x 96-well PCR plates
12
1105154 ID3EAL miRNA Knowledge Panel 96 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
24
1105261 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (2) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
8 x 96-well PCR plates
2
1105262 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (6) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
24 x 96-well PCR plates
6
1105263 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (12) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
48 x 96-well PCR plates
12
1105264 ID3EAL miRNA Knowledge Panel 384 Targets, 96-well (24) 2 x 24 μl of ID3EAL miRNA RT Primers 96-plex
96 x 96-well PCR plates
24


For Purchase Calculation please download Knowledge Panel calculator here:

Knowledge Panel calculator

Choose your thermal cycler

  • Roche
  • Abi Fast
  • Abi Standard
  • Bio-Rad

Cancer miRNA Panel Analysis Template