Select country change
Shopping cart (0 , 0,00 ) Menu Search
Manufactured by BioVendor

Testosterone Mouse/Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Mouse, Rat
Please select your region to see available products and prices.
Cat. No. Size Price


RTC001R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum, Plasma

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–25 ng/ml

Limit of Detection

0.066 ng/ml

Summary

Research topic

Steroid hormones, Animal studies

Summary

Testosterone is the most important male sex hormone, it is responsible for genital development, beard growth, muscle development and general male characteristics. The measurement of serum or plasma levels is an index of leydig cell function and high or low values correlate well with hypo or hyper gonadism. In females small amounts of testosterone are produced by the adrenals and ovaries. High levels of testosterone in females indicates excessive androgen production and are found in progressive hirsutism and virilization, Cushing’s syndrome and a deficiency in one or more of the specific enzymes required for normal steroid biosynthesis.

Product References (15)

References

  • Awodele O, Momoh AA, Awolola NA, Kale OE, Okunowo WO. The combined fixed-dose antituberculous drugs alter some reproductive functions with oxidative stressinvolvement in wistar rats. Toxicol Rep. 2016 Jul 19;3:620-627. doi:10.1016/j.toxrep.2016.06.007. eCollection 2016. PubMed PMID: 28959585; PubMedCentral PMCID: PMC5615933. See more on PubMed
  • Awodele O, Badru WA, Busari AA, Kale OE, Ajayi TB, Udeh RO, Emeka PM.Toxicological evaluation of therapeutic and supra-therapeutic doses ofCellgevity® on reproductive function and biochemical indices in Wistar rats. BMC Pharmacol Toxicol. 2018 Oct 25;19(1):68. doi: 10.1186/s40360-018-0253-y. PubMedPMID: 30359320; PubMed Central PMCID: PMC6202817. See more on PubMed
  • Bae WJ, Zhu GQ, Choi SW, Jeong HC, Bashraheel F, Kim KS, Kim SJ, Cho HJ, HaUS, Hong SH, Lee JY, Oh HA, Koo HC, Kim DR, Hwang SY, Kim SW. Antioxidant andAntifibrotic Effect of a Herbal Formulation In Vitro and in the ExperimentalAndropause via Nrf2/HO-1 Signaling Pathway. Oxid Med Cell Longev.2017;2017:6024839. doi: 10.1155/2017/6024839. Epub 2017 Sep 17. PubMed PMID:29075365; PubMed Central PMCID: PMC5623782. See more on PubMed
  • Dkhil MA, Zrieq R, Al-Quraishy S, Abdel Moneim AE. Selenium NanoparticlesAttenuate Oxidative Stress and Testicular Damage in Streptozotocin-InducedDiabetic Rats. Molecules. 2016 Nov 19;21(11). pii: E1517. PubMed PMID: 27869771; PubMed Central PMCID: PMC6274080. See more on PubMed
  • Garza-Contreras J, Duong P, Snyder BD, Schreihofer DA, Cunningham RL. Presenceof Androgen Receptor Variant in Neuronal Lipid Rafts. eNeuro. 2017 Aug 29;4(4).pii: ENEURO.0109-17.2017. doi: 10.1523/ENEURO.0109-17.2017. eCollection 2017Jul-Aug. PubMed PMID: 28856243; PubMed Central PMCID: PMC5575139. See more on PubMed
  • Choi SW, Jeon SH, Kwon EB, Zhu GQ, Lee KW, Choi JB, Jeong HC, Kim KS, Bae SR, Bae WJ, Kim SJ, Cho HJ, Ha US, Hong SH, Hwang SY, Kim SW. Effect of Korean HerbalFormula (Modified Ojayeonjonghwan) on Androgen Receptor Expression in an AgingRat Model of Late Onset Hypogonadism. World J Mens Health. 2019Jan;37(1):105-112. doi: 10.5534/wjmh.180051. PubMed PMID: 30584994; PubMedCentral PMCID: PMC6305862. See more on PubMed
  • Jayusman PA, Mohamed IN, Alias E, Mohamed N, Shuid AN. The Effects ofQuassinoid-Rich Eurycoma longifolia Extract on Bone Turnover and HistomorphometryIndices in the Androgen-Deficient Osteoporosis Rat Model. Nutrients. 2018 Jun21;10(7). pii: E799. doi: 10.3390/nu10070799. PubMed PMID: 29933617; PubMedCentral PMCID: PMC6073572. See more on PubMed
  • Jayusman PA, Mohamed IN, Shuid AN. The Effects of Chemical Castration withDegarelix on Bone Turnover: Densitometric and Biomechanics Bone Properties ofMale Rats. Int J Endocrinol Metab. 2018 Jul 2;16(3):e64038. doi:10.5812/ijem.64038. eCollection 2018 Jul. PubMed PMID: 30197659; PubMed CentralPMCID: PMC6113792. See more on PubMed
  • Kaçar S, Şahintürk V, Can B, Musmul A. L-Cysteine Partially Protects AgainstAcrylamide-Induced Testicular Toxicity. Balkan Med J. 2018 Apr 4. doi:10.4274/balkanmedj.2017.0830. [Epub ahead of print] PubMed PMID: 29636310; PubMedCentral PMCID: PMC6060964. See more on PubMed
  • Kobyliak NM, Falalyeyeva TM, Kuryk OG, Beregova TV, Bodnar PM, Zholobak NM,Shcherbakov OB, Bubnov RV, Spivak MY. Antioxidative effects of cerium dioxidenanoparticles ameliorate age-related male infertility: optimistic results in ratsand the review of clinical clues for integrative concept of men health andfertility. EPMA J. 2015 Jun 10;6(1):12. doi: 10.1186/s13167-015-0034-2.eCollection 2015. PubMed PMID: 26097523; PubMed Central PMCID: PMC4475301. See more on PubMed
  • Makar AB, McMartin KE, Palese M, Tephly TR. Formate assay in body fluids:application in methanol poisoning. Biochem Med. 1975 Jun;13(2):117-26. PubMedPMID: 1. See more on PubMed
  • Skovmand A, Jacobsen Lauvås A, Christensen P, Vogel U, Sørig Hougaard K,Goericke-Pesch S. Pulmonary exposure to carbonaceous nanomaterials and spermquality. Part Fibre Toxicol. 2018 Jan 31;15(1):10. doi:10.1186/s12989-018-0242-8. PubMed PMID: 29386028; PubMed Central PMCID:PMC5793436. See more on PubMed
  • Awodele O, Kale OE, Odewabi AO, Ekor M, Salau BA, Adefule-Ositelu AO. Safetyevaluation of Bon-santé cleanser(®) polyherbal in male Wistar rats: Furtherinvestigations on androgenic and toxicological profile. J Tradit Complement Med. 2017 Jun 20;8(1):212-219. doi: 10.1016/j.jtcme.2017.06.002. eCollection 2018 Jan.PubMed PMID: 29322011; PubMed Central PMCID: PMC5756022. See more on PubMed
  • Wilson EN, Anderson M, Snyder B, Duong P, Trieu J, Schreihofer DA, Cunningham RL. Chronic intermittent hypoxia induces hormonal and male sexual behavioralchanges: Hypoxia as an advancer of aging. Physiol Behav. 2018 May 15;189:64-73.doi: 10.1016/j.physbeh.2018.03.007. Epub 2018 Mar 8. PubMed PMID: 29526572;PubMed Central PMCID: PMC5882542. See more on PubMed
  • Skovmand A, Jensen ACØ, Maurice C, Marchetti F, Lauvås AJ, Koponen IK, Jensen KA, Goericke-Pesch S, Vogel U, Hougaard KS. Effects of maternal inhalation ofcarbon black nanoparticles on reproductive and fertility parameters in afour-generation study of male mice. Part Fibre Toxicol. 2019 Mar 18;16(1):13.doi: 10.1186/s12989-019-0295-3. PubMed PMID: 30879468; PubMed Central PMCID:PMC6421671. See more on PubMed
Summary References (16)

References to Testosterone

  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Dechaud H, Lejeune H, Garoscio-Cholet M, Mallein R, Pugeat M. Radioimmunoassay of testosterone not bound to sex-steroid-binding protein in plasma. Clin Chem. 1989 Aug;35 (8):1609-14
  • Ekins R. Hirsutism: free and bound testosterone. Ann Clin Biochem. 1990 Jan;27 ( Pt 1):91-4
  • Joshi UM, Shah HP, Sudhama SP. A sensitive and specific enzymeimmunoassay for serum testosterone. Steroids. 1979 Jul;34 (1):35-46
  • Manni A, Pardridge WM, Cefalu W, Nisula BC, Bardin CW, Santner SJ, Santen RJ. Bioavailability of albumin-bound testosterone. J Clin Endocrinol Metab. 1985 Oct;61 (4):705-10
  • Marcus GJ, Durnford R. A simple enzyme-linked immunosorbent assay for testosterone. Steroids. 1985 Dec;46 (6):975-86
  • Oka K, Hirano T, Noguchi M. Changes in the concentration of testosterone in serum during the menstrual cycle, as determined by liquid chromatography. Clin Chem. 1988 Mar;34 (3):557-60
  • Palatsi R, Hirvensalo E, Liukko P, Malmiharju T, Mattila L, Riihiluoma P, Ylostalo P. Serum total and unbound testosterone and sex hormone binding globulin (SHBG) in female acne patients treated with two different oral contraceptives. Acta Derm Venereol. 1984;64 (6):517-23
  • Pardridge WM, Demers LM. Bioavailable testosterone in salivary glands. Clin Chem. 1991 Feb;37 (2):139-40
  • Rassaie MJ, Kumari GL, Rao PN, Shrivastav TG, Pandey HP. Influence of different combinations of antibodies and penicillinase-labeled testosterone derivatives on sensitivity and specificity of immunoassays. Steroids. 1992 Mar;57 (3):112-8
  • Sheldon PE, Coppenger CJ. A rapid radioimmunoassay for serum testosterone. Steroids. 1977 Aug;30 (2):149-57
  • Singer F, Zumoff B. Subnormal serum testosterone levels in male internal medicine residents. Steroids. 1992 Feb;57 (2):86-9
  • Swinkels LM, Meulenberg PM, Ross HA, Benraad TJ. Salivary and plasma free testosterone and androstenedione levels in women using oral contraceptives containing desogestrel or levonorgestrel. Ann Clin Biochem. 1988 Jul;25 ( Pt 4):354-9
  • Swinkels LM, Ross HA, Benraad TJ. A symmetric dialysis method for the determination of free testosterone in human plasma. Clin Chim Acta. 1987 Jun 15;165 (2-3):341-9
  • Tateiki, K., et al. Enzymeimmunoassay of serum Testosterone. Steroids. 30:25, 1977;
  • Wilke TJ, Utley DJ. Total testosterone, free-androgen index, calculated free testosterone, and free testosterone by analog RIA compared in hirsute women and in otherwise-normal women with altered binding of sex-hormone-binding globulin. Clin Chem. 1987 Aug;33 (8):1372-5
Related Products Docs