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Manufactured by BioVendor

Testosterone Mouse/Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antigen
  • Species:Mouse, Rat
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Cat. No. Size Price


RTC001R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antigen

Applications

Serum, Plasma

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.1–25 ng/ml

Limit of Detection

0.066 ng/ml

Summary

Research topic

Steroid hormones, Animal studies

Summary

Testosterone is the most important male sex hormone, it is responsible for genital development, beard growth, muscle development and general male characteristics. The measurement of serum or plasma levels is an index of leydig cell function and high or low values correlate well with hypo or hyper gonadism. In females small amounts of testosterone are produced by the adrenals and ovaries. High levels of testosterone in females indicates excessive androgen production and are found in progressive hirsutism and virilization, Cushing’s syndrome and a deficiency in one or more of the specific enzymes required for normal steroid biosynthesis.

References to Product

References

  • Awodele O, Momoh AA, Awolola NA, Kale OE. The Combined Fixed-Dose Antituberculous Drugs Alter Some Reproductive Functions with oxidative stress involvement in Wistar Rats. Toxicology reports. July 2016;
  • Awodele O, Badru WA, Busari AA, et al. Toxicological evaluation of therapeutic and supra-therapeutic doses of Cellgevity® on reproductive function and biochemical indices in Wistar rats. BMC Pharmacol Toxicol. 2018;19(1):68. Published 2018 Oct 25. doi:10.1186/s40360-018-0253-y
  • Bae WJ, Zhu GQ, Choi SW, et al. Antioxidant and Antifibrotic Effect of a Herbal Formulation In Vitro and in the Experimental Andropause via Nrf2/HO-1 Signaling Pathway. Oxid Med Cell Longev. 2017;2017:6024839.
  • Dkhil MA, Zrieq R, Al-Quraishy S, Abdel Moneim AE. Selenium Nanoparticles Attenuate Oxidative Stress and Testicular Damage in Streptozotocin-Induced Diabetic Rats. Molecules. 2016;21(11):1517. Published 2016 Nov 19. doi:10.3390/molecules21111517
  • Garza-Contreras J, Duong P, Snyder BD, Schreihofer DA, Cunningham RL. Presence of Androgen Receptor Variant in Neuronal Lipid Rafts. eNeuro. 2017;4(4):ENEURO.0109-17.2017. Published 2017 Aug 29. doi:10.1523/ENEURO.0109-17.2017
  • Choi SW, Jeon SH, Kwon EB, et al. Effect of Korean Herbal Formula (Modified Ojayeonjonghwan) on Androgen Receptor Expression in an Aging Rat Model of Late Onset Hypogonadism. World J Mens Health. 2018;37(1):105-112.
  • Jayusman PA, Mohamed IN, Alias E, Mohamed N, Shuid AN. The Effects of Quassinoid-Rich Eurycoma longifolia Extract on Bone Turnover and Histomorphometry Indices in the Androgen-Deficient Osteoporosis Rat Model. Nutrients. 2018;10(7):799. Published 2018 Jun 21. doi:10.3390/nu10070799
  • Jayusman PA, Mohamed IN, Shuid AN. The Effects of Chemical Castration with Degarelix on Bone Turnover: Densitometric and Biomechanics Bone Properties of Male Rats. Int J Endocrinol Metab. 2018;16(3):e64038. Published 2018 Jul 2. doi:10.5812/ijem.64038
  • Kaçar S, Şahintürk V, Can B, Musmul A. L-Cysteine Partially Protects Against Acrylamide-Induced Testicular Toxicity[published online ahead of print, 2018 Jul 24]. Balkan Med J. 2018;35(4):311–319. doi:10.4274/balkanmedj.2017.0830
  • Kobyliak NM, Falalyeyeva TM, Kuryk OG, et al. Antioxidative effects of cerium dioxide nanoparticles ameliorate age-related male infertility: optimistic results in rats and the review of clinical clues for integrative concept of men health and fertility. EPMA J. 2015;6(1):12. Published 2015 Jun 10. doi:10.1186/s13167-015-0034-2
  • Makar AB, McMartin KE, Palese M, Tephly TR. Formate assay in body fluids: application in methanol poisoning. Biochem Med. 1975 Jun;13 (2):117-26
  • Skovmand A, Jacobsen Lauvås A, Christensen P, Vogel U, Sørig Hougaard K, Goericke-Pesch S. Pulmonary exposure to carbonaceous nanomaterials and sperm quality. Part Fibre Toxicol. 2018;15(1):10. Published 2018 Jan 31. doi:10.1186/s12989-018-0242-8
References to Summary

References to Testosterone

  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Dechaud H, Lejeune H, Garoscio-Cholet M, Mallein R, Pugeat M. Radioimmunoassay of testosterone not bound to sex-steroid-binding protein in plasma. Clin Chem. 1989 Aug;35 (8):1609-14
  • Ekins R. Hirsutism: free and bound testosterone. Ann Clin Biochem. 1990 Jan;27 ( Pt 1):91-4
  • Joshi UM, Shah HP, Sudhama SP. A sensitive and specific enzymeimmunoassay for serum testosterone. Steroids. 1979 Jul;34 (1):35-46
  • Manni A, Pardridge WM, Cefalu W, Nisula BC, Bardin CW, Santner SJ, Santen RJ. Bioavailability of albumin-bound testosterone. J Clin Endocrinol Metab. 1985 Oct;61 (4):705-10
  • Marcus GJ, Durnford R. A simple enzyme-linked immunosorbent assay for testosterone. Steroids. 1985 Dec;46 (6):975-86
  • Oka K, Hirano T, Noguchi M. Changes in the concentration of testosterone in serum during the menstrual cycle, as determined by liquid chromatography. Clin Chem. 1988 Mar;34 (3):557-60
  • Palatsi R, Hirvensalo E, Liukko P, Malmiharju T, Mattila L, Riihiluoma P, Ylostalo P. Serum total and unbound testosterone and sex hormone binding globulin (SHBG) in female acne patients treated with two different oral contraceptives. Acta Derm Venereol. 1984;64 (6):517-23
  • Pardridge WM, Demers LM. Bioavailable testosterone in salivary glands. Clin Chem. 1991 Feb;37 (2):139-40
  • Rassaie MJ, Kumari GL, Rao PN, Shrivastav TG, Pandey HP. Influence of different combinations of antibodies and penicillinase-labeled testosterone derivatives on sensitivity and specificity of immunoassays. Steroids. 1992 Mar;57 (3):112-8
  • Sheldon PE, Coppenger CJ. A rapid radioimmunoassay for serum testosterone. Steroids. 1977 Aug;30 (2):149-57
  • Singer F, Zumoff B. Subnormal serum testosterone levels in male internal medicine residents. Steroids. 1992 Feb;57 (2):86-9
  • Swinkels LM, Meulenberg PM, Ross HA, Benraad TJ. Salivary and plasma free testosterone and androstenedione levels in women using oral contraceptives containing desogestrel or levonorgestrel. Ann Clin Biochem. 1988 Jul;25 ( Pt 4):354-9
  • Swinkels LM, Ross HA, Benraad TJ. A symmetric dialysis method for the determination of free testosterone in human plasma. Clin Chim Acta. 1987 Jun 15;165 (2-3):341-9
  • Tateiki, K., et al. Enzymeimmunoassay of serum Testosterone. Steroids. 30:25, 1977;
  • Wilke TJ, Utley DJ. Total testosterone, free-androgen index, calculated free testosterone, and free testosterone by analog RIA compared in hirsute women and in otherwise-normal women with altered binding of sex-hormone-binding globulin. Clin Chem. 1987 Aug;33 (8):1372-5
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