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Thyroid Stimulating Hormone Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:TSH, Thyrotropin
  • Species:Rat
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Cat. No. Size Price


RTC007R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2.5–80 ng/ml

Limit of Detection

0.081 ng/ml

Summary

Research topic

Animal studies

Summary

Thyroid stimulating hormone (TSH) is a glycoprotein hormone secreted by the anterior pituitary gland. TSH has two subunits, namely α and β. The α subunit of TSH is similar to the α subunit found in the LH, FSH and hCG glycoprotein hormones. However, the β subunit is specific and differs from hormone to hormone. The thyroid hormones are secreted and produced by the thyroid gland. The production of thyroid hormones is under the regulation of TSH. Also, TSH acts as a stimulator of iodide transport and the gland itself is under the positive control of TSH. The concentrations of thyroid hormones control the secretion of TSH, therefore, a negative feedback exists. It is to be noted that the secretion of thyroid hormones are under the direct, positive effect of the sympathetic nervous system. The major protein component of the thyroid gland is thyroglobulin, a glycoprotein of which the secretion in the blood stream is stimulated by TSH. Therefore, TSH plays an important role in the proper function and development of the thyroid gland. It is recommended to assay both the glycoprotein hormone and the target organ hormones. For example, in primary hypothyroidism the serum level of thyroxine is low while the TSH level is high. In secondary hypothyroidism, both thyroxine and TSH are low. The TSH level is decreased in hyperthyroidism. Today, with all the sensitive assays available, if there were to be only one test to be prescribed for thyroid function, TSH would be the test. TSH determinations are also helpful to monitor patients who receive thyroxine replacement therapy.

References to Summary

References to Thyroid Stimulating Hormone

  • Allen KR, Scott RD, Hewitt JV, Watson D. Clinical value of a sensitive immunoradiometric assay for TSH. Ann Clin Biochem. 1985 Sep;22 ( Pt 5):506-8
  • Benkirane M, Bon D, Bellot F, Prince P, Hassoun J, Carayon P, Delori P. Characterization of monoclonal antibodies against human thyrotropin and use in an immunoradiometric assay and immunohistochemistry. J Immunol Methods. 1987 Apr 16;98 (2):173-81
  • Carayon P, Guibout M, Lissitzky S. The interaction of radioiodinated thyrotropin with human plasma membranes from normal and diseased thyroid glands. Relation of thyrotropin binding to adenylate cyclase activity. Ann Endocrinol (Paris). 1979;40 (3):211-27
  • Carayon P, Martino E, Bartalena L, Grasso L, Mammoli C, Costagliola S, Pinchera A. Clinical usefulness and limitations of serum thyrotropin measurement by 'ultrasensitive' methods. Comparisons of five kits. Horm Res. 1987;26 (1-4):105-17
  • Clark PM, Price CP. Enzyme-amplified immunoassays: a new ultrasensitive assay of thyrotropin evaluated. Clin Chem. 1986 Jan;32 (1 Pt 1):88-92
  • Cornell JS, Pierce JG. The subunits of human pituitary thyroid-stimulating hormone. Isolation, properties, and composition. J Biol Chem. 1973 Jun 25;248 (12):4327-33
  • Cusick CF, Mistry K, Addison GM. Interference in a two-site immunoradiometric assay for thyrotropin in a child. Clin Chem. 1985 Feb;31 (2):348-9
  • Dumont JE. The action of thyrotropin on thyroid metabolism. Vitam Horm. 1971;29:287-412
  • Evans M, Croxson MS, Wilson TM, Ibbertson HK. The screening of patients with suspected thyrotoxicosis using a sensitive TSH radioimmunoassay. Clin Endocrinol (Oxf). 1985 Apr;22 (4):445-51
  • Greenspan FS, Lew W, Okerlund MD, Lowenstein JM. Falsely positive bovine TSH radioimmunoassay responses in sera from patients with thyroid cancer. J Clin Endocrinol Metab. 1974 Jun;38 (6):1121-2
  • Hall R, Amos J, Ormston BJ. Radioimmunoassay of human serum thyrotrophin. Br Med J. 1971 Mar 13;1 (5749):582-5
  • Howanitz PJ, Howanitz JH, Lamberson HV, Ennis KM. Incidence and mechanism of spurious increase in serum thyrotropin. Clin Chem. 1982 Mar;28 (3):427-31
  • Malter JS, Manotti SE, Knee GR, Goodman DB, Strauss JF 3rd. Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin. Clin Chem. 1985 Apr;31 (4):642-4
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