United States set
Menu Shopping cart $0 Search
Manufactured by BioVendor

Rat Thyroid Stimulating Hormone ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Other names:TSH, Thyrotropin
  • Species:Rat
Cat. No. Size Price

RTC007R 96 wells (1 kit) $570,15
PubMed Product Details
Technical Data


Sandwich ELISA, HRP-labelled antibody


The BioVendor Rat TSH ELISA is an enzyme immunoassay for the quantitative measurement of TSH in rat serum. For research use only. Not for use in diagnostic procedures.



Sample Requirements

25 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

2.5–80 ng/ml

Limit of Detection

0.081 ng/ml



  • RUO
  • calibration range 2.5-80 ng/ml
  • limit of detection 0.081 ng/ml

Research topic

Animal studies


Thyroid stimulating hormone (also known as thyrotropin or TSH) is a glycoprotein produced by the anterior pituitary gland. Through its action on the thyroid gland, it plays a major role in maintaining normal circulating levels of the iodothyronines, T4 and T3. The production and secretion of TSH is controlled on the one side by negative feedback from circulating T4 and T3, and on the other side by the hypothalamic thyrotropin-releasing hormone (TRH). The TSH molecule is composed of two non-identical subunits, α and β, that are bound together in a noncovalent manner. Within a species, the TSH α unit is structurally identical to the α subunits of related glycoprotein hormones (LH, FSH). The β subunits of the related hormones are structurally hormone-specific and therefore determine their unique biological activities. The mechanism controlling thyroid function in rats is exactly analogous to the mechanism operating in humans. This means that thyrotropin-releasing hormone stimulates the release of TSH from the pituitary gland as well as the serum concentrations of T4 and T3 influence the action of the pituitary gland. This similarity between rat and human thyroid physiology makes the rat a very useful model for evaluating the effects of new drugs on thyrometabolic status.

Summary References (13)

References to Thyroid Stimulating Hormone

  • Allen KR, Scott RD, Hewitt JV, Watson D. Clinical value of a sensitive immunoradiometric assay for TSH. Ann Clin Biochem. 1985 Sep;22 ( Pt 5):506-8
  • Benkirane M, Bon D, Bellot F, Prince P, Hassoun J, Carayon P, Delori P. Characterization of monoclonal antibodies against human thyrotropin and use in an immunoradiometric assay and immunohistochemistry. J Immunol Methods. 1987 Apr 16;98 (2):173-81
  • Carayon P, Guibout M, Lissitzky S. The interaction of radioiodinated thyrotropin with human plasma membranes from normal and diseased thyroid glands. Relation of thyrotropin binding to adenylate cyclase activity. Ann Endocrinol (Paris). 1979;40 (3):211-27
  • Carayon P, Martino E, Bartalena L, Grasso L, Mammoli C, Costagliola S, Pinchera A. Clinical usefulness and limitations of serum thyrotropin measurement by 'ultrasensitive' methods. Comparisons of five kits. Horm Res. 1987;26 (1-4):105-17
  • Clark PM, Price CP. Enzyme-amplified immunoassays: a new ultrasensitive assay of thyrotropin evaluated. Clin Chem. 1986 Jan;32 (1 Pt 1):88-92
  • Cornell JS, Pierce JG. The subunits of human pituitary thyroid-stimulating hormone. Isolation, properties, and composition. J Biol Chem. 1973 Jun 25;248 (12):4327-33
  • Cusick CF, Mistry K, Addison GM. Interference in a two-site immunoradiometric assay for thyrotropin in a child. Clin Chem. 1985 Feb;31 (2):348-9
  • Dumont JE. The action of thyrotropin on thyroid metabolism. Vitam Horm. 1971;29:287-412
  • Evans M, Croxson MS, Wilson TM, Ibbertson HK. The screening of patients with suspected thyrotoxicosis using a sensitive TSH radioimmunoassay. Clin Endocrinol (Oxf). 1985 Apr;22 (4):445-51
  • Greenspan FS, Lew W, Okerlund MD, Lowenstein JM. Falsely positive bovine TSH radioimmunoassay responses in sera from patients with thyroid cancer. J Clin Endocrinol Metab. 1974 Jun;38 (6):1121-2
  • Hall R, Amos J, Ormston BJ. Radioimmunoassay of human serum thyrotrophin. Br Med J. 1971 Mar 13;1 (5749):582-5
  • Howanitz PJ, Howanitz JH, Lamberson HV, Ennis KM. Incidence and mechanism of spurious increase in serum thyrotropin. Clin Chem. 1982 Mar;28 (3):427-31
  • Malter JS, Manotti SE, Knee GR, Goodman DB, Strauss JF 3rd. Identification of hyperthyroid patients by means of a sensitive assay for thyrotropin. Clin Chem. 1985 Apr;31 (4):642-4
Related Products Docs