United States set
Menu Shopping cart $0 Search
Manufactured by BioVendor

Mouse TNF-alpha ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Cachectin, Tumor necrosis factor alpha, DIF
  • Species:Mouse
This product is not available in United States!
Cat. No. Size Price

RAF129R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


The mouse TNF-α ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TNF-α. Cell culture supernatant, serum, and plasma (EDTA, citrate) have been tested with this assay.


Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well


On blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

31.3–2000 pg/ml

Limit of Detection

3.7 pg/ml

Intra-assay (Within-Run)

CV = 6.5%

Inter-assay (Run-to-Run)

CV = 5.7%

Spiking Recovery


Dilution Linearity



The assay detects both natural and recombinant mouse TNF-α. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into mouse IL-10 positive serum. There was no crossreactivity detected.



  • RUO
  • calibration range 31.3-2000 pg/ml
  • limit of detection 3.7 pg/ml
  • intra-assay CV = 6.5%
  • inter-assay CV = 5.7%

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Animal studies


TNF-α is a multifunctional cytokine involved in many different pathways, in homeostasis and pathophysiology of mammals. It can show opposing biological effects suggesting complex regulatory mechanisms. TNF-α, also known as cachectin, was first detected as a cytotoxic factor inducing lysis of certain tumor cells. The TNF-α gene is member 2 of the TNF-superfamily (consisting of at least 20 distinct members).

Product References (1)


  • Cavaliere G, Trinchese G, Penna E, Cimmino F, Pirozzi C, Lama A, Annunziata C, Catapano A, Mattace Raso G, Meli R, Monda M, Messina G, Zammit C, Crispino M, Mollica MP. High-Fat Diet Induces Neuroinflammation and Mitochondrial Impairment in Mice Cerebral Cortex and Synaptic Fraction. Front Cell Neurosci. 2019 Nov 12;13:509. doi: 10.3389/fncel.2019.00509. eCollection 2019. PubMed PMID: 31798417. PubMed CentralPMCID: PMC6861522. See more on PubMed
Related Products Docs