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Manufactured by BioVendor

TNF-alpha Mouse ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Cachectin, Tumor necrosis factor alpha, DIF
  • Species:Mouse
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Cat. No. Size Price


RAF129R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

31.3–2000 pg/mL

Limit of Detection

3.7 pg/ml

Intra-assay (Within-Run)

CV = 6.5%

Inter-assay (Run-to-Run)

CV = 5.7%

Spiking Recovery

97,00%

Dilution Linearity

94,00%

Summary

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Animal studies

Summary

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

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