Type
Sandwich ELISA, Biotin-labelled antibody
Description
The mouse TNF-α ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse TNF-α. Cell culture supernatant, serum, and plasma (EDTA, citrate) have been tested with this assay.
Applications
Serum, Plasma, Cell culture supernatant
Sample Requirements
50 µl/well
Shipping
On blue ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
31.3–2000 pg/ml
Limit of Detection
3.7 pg/ml
Intra-assay (Within-Run)
CV = 6.5%
Inter-assay (Run-to-Run)
CV = 5.7%
Spiking Recovery
97,00%
Dilution Linearity
94,00%
Specificity
The assay detects both natural and recombinant mouse TNF-α. The interference of circulating factors of the immune systeme was evaluated by spiking these proteins at physiologically relevant concentrations into mouse IL-10 positive serum. There was no crossreactivity detected.