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TNF-alpha Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Cachectin, Tumor necrosis factor alpha, DIF
  • Species:Rat
Cat. No. Size Price

RAF130R 96 wells (1 kit) $694,89
PubMed Product Details
Technical Data


Sandwich ELISA, Biotin-labelled antibody


Serum, Cell culture supernatant

Sample Requirements

50 µl/well


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

39.1–2500 pg/ml

Limit of Detection

11 pg/ml

Intra-assay (Within-Run)

CV = 5.0%

Inter-assay (Run-to-Run)

CV = 10.0%

Spiking Recovery


Dilution Linearity



Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Animal studies


TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Product References (5)


  • Trinchese G, Cavaliere G, Penna E, De Filippo C, Cimmino F, Catapano A, Musco N, Tudisco R, Lombardi P, Infascelli F, Messina G, Muredda L, Banni S, Monda M, Crispino M, Mollica MP. Milk From Cow Fed With High Forage/Concentrate Ratio Diet: Beneficial Effect on Rat Skeletal Muscle Inflammatory State and Oxidative Stress Through Modulation of Mitochondrial Functions and AMPK Activity. Front Physiol. 2019 Jan 17;9:1969. doi: 10.3389/fphys.2018.01969. eCollection 2018. PubMed PMID: 30705640. PubMed CentralPMCID: PMC6344429. See more on PubMed
  • Cavaliere G, Viggiano E, Trinchese G, De Filippo C, Messina A, Monda V, Valenzano A, Cincione RI, Zammit C, Cimmino F, Catapano A, Sessa F, Messina G, Monda M, Crispino M, Mollica MP. Long Feeding High-Fat Diet Induces Hypothalamic Oxidative Stress and Inflammation, and Prolonged Hypothalamic AMPK Activation in Rat Animal Model. Front Physiol. 2018 Jul 6;9:818. doi: 10.3389/fphys.2018.00818. eCollection 2018. PubMed PMID: 30034345. PubMed CentralPMCID: PMC6043859. See more on PubMed
  • Trinchese G, Cavaliere G, De Filippo C, Aceto S, Prisco M, Chun JT, Penna E, Negri R, Muredda L, Demurtas A, Banni S, Berni-Canani R, Mattace Raso G, Calignano A, Meli R, Greco L, Crispino M, Mollica MP. Human Milk and Donkey Milk, Compared to Cow Milk, Reduce Inflammatory Mediators and Modulate Glucose and Lipid Metabolism, Acting on Mitochondrial Function and Oleylethanolamide Levels in Rat Skeletal Muscle. Front Physiol. 2018 Jan 30;9:32. doi: 10.3389/fphys.2018.00032. eCollection 2018. PubMed PMID: 29472867. PubMed CentralPMCID: PMC5810302. See more on PubMed
  • Chiorescu S, Andercou OA, Grad NO, Mironiuc IA. Intraperitoneal administration of rosuvastatin prevents postoperative peritoneal adhesions by decreasing the release of tumor necrosis factor. Clujul Med. 2018;91(1):79-84. doi: 10.15386/cjmed-859. Epub 2018 Jan 15. PubMed PMID: 29440955. PubMed CentralPMCID: PMC5808273. See more on PubMed
  • Peric Kacarevic Z, Snajder D, Maric A, Bijelic N, Cvijanovic O, Domitrovic R, Radic R. High-fat diet induced changes in lumbar vertebra of the male rat offsprings. Acta Histochem. 2016 Sep;118(7):711-721. doi: 10.1016/j.acthis.2016.08.002. Epub 2016 Aug 28. PubMed PMID: 27577321. See more on PubMed
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