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Manufactured by BioVendor

TNF-alpha Rat ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Cachectin, Tumor necrosis factor alpha, DIF
  • Species:Rat
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Cat. No. Size Price


RAF130R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Cell culture supernatant

Sample Requirements

50 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

39.1–2500 pg/ml

Limit of Detection

11 pg/ml

Intra-assay (Within-Run)

CV = 5.0%

Inter-assay (Run-to-Run)

CV = 10.0%

Spiking Recovery

109,00%

Dilutation Linearity

114,00%

Summary

Research topic

Cytokines and chemokines and related molecules, Energy metabolism and body weight regulation, Animal studies

Summary

TNF-α (17.5 kDa, 157 amino acid) is a non-glycosylated protein that is a potent lymphoid factor, which exerts cytotoxic effects on a wide range of tumor cells and other target cells. It is secreted by macrophages, monocytes, neutrophils, T-cells and NK-cells following their stimulation by bacterial lipopolysaccha­rides. TNF-α has been suggested to play a pro-inflammatory role and has been detected in synovial fluid of patients with rheumatoid arthritis.

Product References (5)

References

  • Cavaliere G, Viggiano E, Trinchese G, De Filippo C, Messina A, Monda V,Valenzano A, Cincione RI, Zammit C, Cimmino F, Catapano A, Sessa F, Messina G,Monda M, Crispino M, Mollica MP. Long Feeding High-Fat Diet Induces Hypothalamic Oxidative Stress and Inflammation, and Prolonged Hypothalamic AMPK Activation in Rat Animal Model. Front Physiol. 2018 Jul 6;9:818. doi: 10.3389/fphys.2018.00818.eCollection 2018. PubMed PMID: 30034345; PubMed Central PMCID: PMC6043859. See more on PubMed
  • Chiorescu S, Andercou OA, Grad NO, Mironiuc IA. Intraperitoneal administrationof rosuvastatin prevents postoperative peritoneal adhesions by decreasing therelease of tumor necrosis factor. Clujul Med. 2018;91(1):79-84. doi:10.15386/cjmed-859. Epub 2018 Jan 15. PubMed PMID: 29440955; PubMed CentralPMCID: PMC5808273. See more on PubMed
  • Peric Kacarevic Z, Snajder D, Maric A, Bijelic N, Cvijanovic O, Domitrovic R, Radic R. High-fat diet induced changes in lumbar vertebra of the male ratoffsprings. Acta Histochem. 2016 Sep;118(7):711-721. doi:10.1016/j.acthis.2016.08.002. Epub 2016 Aug 28. PubMed PMID: 27577321. See more on PubMed
  • Trinchese G, Cavaliere G, De Filippo C, Aceto S, Prisco M, Chun JT, Penna E,Negri R, Muredda L, Demurtas A, Banni S, Berni-Canani R, Mattace Raso G,Calignano A, Meli R, Greco L, Crispino M, Mollica MP. Human Milk and Donkey Milk,Compared to Cow Milk, Reduce Inflammatory Mediators and Modulate Glucose andLipid Metabolism, Acting on Mitochondrial Function and Oleylethanolamide Levelsin Rat Skeletal Muscle. Front Physiol. 2018 Jan 30;9:32. doi:10.3389/fphys.2018.00032. eCollection 2018. PubMed PMID: 29472867; PubMed CentralPMCID: PMC5810302. See more on PubMed
  • Trinchese G, Cavaliere G, Penna E, De Filippo C, Cimmino F, Catapano A, Musco N, Tudisco R, Lombardi P, Infascelli F, Messina G, Muredda L, Banni S, Monda M,Crispino M, Mollica MP. Milk From Cow Fed With High Forage/Concentrate RatioDiet: Beneficial Effect on Rat Skeletal Muscle Inflammatory State and OxidativeStress Through Modulation of Mitochondrial Functions and AMPK Activity. FrontPhysiol. 2019 Jan 17;9:1969. doi: 10.3389/fphys.2018.01969. eCollection 2018.PubMed PMID: 30705640; PubMed Central PMCID: PMC6344429. See more on PubMed
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