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Two-Tailed qPCR: Advantages

  • Highly sensitive (up to less than 10 molecules)
  • miRNA detection and quantification in wide spectrum of materials (plasma/serum, blood, tissues, …)
  • Very high specificity
  • Exceeding performance compared to other RT-qPCR techniques
  • superior solution of older RT-qPRC techniques insufficiencies
  • Two hemiprobes, which bind to different stretches of the microRNA, that are connected by a folded tether
  • both hemiprobes are complementary and they bind cooperatively
  • Wide dynamic range (up to 9 log)
  • High degree two-tube multiplexing of the RT followed by single-plex qPCR
  • Customized assays available

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