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Two-Tailed RT-qPCR

Patented Two-Tailed RT-qPCR technology with a unique mechanism of RT-primers to provide superior performance with excelent specificity in comparison to other methods. Two-Tailed RT-qPCR is standardized for miRNA and piRNA total quantification. 

The challenge detecting small microRNAs is that two conventional PCR primers do not fit the target as their combined length is almost twice the length of the microRNA. Older techniques have solved this by extending the microRNA using e.g., a hairpin primer, adding a poly A-tail, or adding a fragment by ligation. This, however, compromises the assay sensitivity and specificity, as only one of the PCR primers sense the actual microRNA sequence; the other senses the added extension. Further, these methods fail to detect microRNAs modified in the 3’-end as it interferes with the extension process.

13032

The technology has been developed in TATAABiocenter and bought by BioVendor. The miRNA two-Tailed RT-qPCR assays offers a superior solution. Instead of using a single binding probe, Two-tailed PCR uses two hemiprobes, which bind to different stretches of the microRNA, that are connected by a folded tether. While each hemiprobe is too short to bind the microRNA, when both hemiprobes are complementary they bind cooperatively. Binding is exceeding specific, as a mismatch is much more profound in a short hemiprobe. The cDNA formed can then be PCR amplified using two sequence specific primers. SYBR used for detection. High melting resolution analysis can be used for non-specific products detection. 

Customized assays

To place a customized assay order, contact us on the following mail: info@biovendor.com 

 

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Product Size Price
Two-Tailed cDNA Synthesis System 50 rxn

Type: Two-Tailed cDNA Synthesis System

New RDTTRT50 50 rxn
 
Two-Tailed qPCR Master Mix 150 rxn

Type: Two-Tailed qPCR Master Mix

New RDTTPCR150 150 rxn
 
Product Size Price
hsa-miR-122-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000421PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-125a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0004602PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-130a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000425PRI 50 rxn/ 150 rxn
 
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hsa-miR-16-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000069PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-1909-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0007883PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-191-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000440PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-199-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000232PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-20b-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0001413PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-21-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000076PRI 50 rxn/ 150 rxn
 
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hsa-miR-23a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000078PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-29c-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000681PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-301a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000688PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-30b-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000420PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-31-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000089RTPRI 50 rxn/ 150 rxn