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Two-Tailed RT-qPCR

Patented Two-Tailed RT-qPCR technology with a unique mechanism of RT-primers to provide superior performance with excelent specificity in comparison to other methods. Two-Tailed RT-qPCR is standardized for miRNA and piRNA total quantification. 

The challenge detecting small microRNAs is that two conventional PCR primers do not fit the target as their combined length is almost twice the length of the microRNA. Older techniques have solved this by extending the microRNA using e.g., a hairpin primer, adding a poly A-tail, or adding a fragment by ligation. This, however, compromises the assay sensitivity and specificity, as only one of the PCR primers sense the actual microRNA sequence; the other senses the added extension. Further, these methods fail to detect microRNAs modified in the 3’-end as it interferes with the extension process.


TWO-TAILED RT-PCR TECHNIQUE

The technology has been developed in TATAABiocenter and bought by BioVendor. The miRNA two-Tailed RT-qPCR assays offers a superior solution. Instead of using a single binding probe, Two-tailed PCR uses two hemiprobes, which bind to different stretches of the microRNA, that are connected by a folded tether. While each hemiprobe is too short to bind the microRNA, when both hemiprobes are complementary they bind cooperatively. Binding is exceeding specific, as a mismatch is much more profound in a short hemiprobe. The cDNA formed can then be PCR amplified using two sequence specific primers. SYBR used for detection. High melting resolution analysis can be used for non-specific products detection. 

Isolation efficiency? Positive control? Absolute quantification?

Try our NEW Synthetic miRNA molecules. You can now order any synthetic miRNA of your interest!

 

Customized assays

To place a customized assay order, contact us on the following mail: gresakova@biovendor.com

 

Frequently Asked Questions

Browse help topics here or download FAQs in PDF
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Product Size Price
TT synthetic miRNA cel-miR-54-3p

Type: Two-Tailed miRNA synthetic

New TTK00000253 50 reactions
 
Two-Tailed cDNA Synthesis System 50 rxn

Type: Two-Tailed cDNA Synthesis System

New RDTTRT50 50 rxn
 
Two-Tailed qPCR Master Mix 150 rxn

Type: Two-Tailed qPCR Master Mix

New RDTTPCR150 150 rxn
 
Product Size Price
hsa-let-7i-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000415PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-103a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000101PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-106-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000103PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-122-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000421PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-124-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000422PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-125a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0004602PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-126-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000445PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-130a-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000425PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-138-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000430PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-147b-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0004928PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-149-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000450PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-155-3p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0004658PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-155-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000646PRI 50 rxn/ 150 rxn
 
Product Size Price
hsa-miR-15b-5p Two-Tailed PRIMERs

Type: Two-Tailed RT/ PCR Primers

New RDTT0000417PRI 50 rxn/ 150 rxn