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Urocortin 3 Mouse/Rat ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA, Immobilized antibody
  • Other names:Stresscopin, Ucn3, SCP
  • Species:Mouse, Rat
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Cat. No. Size Price


RSCYK200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA, Immobilized antibody

Applications

Serum, Brain extract, Plasma

Sample Requirements

25 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.41–100 ng/ml

Intra-assay (Within-Run)

Mouse plasma: 6.1-12.3%
Mouse serum: 5.1-13.5%
Rat plasma: 10.5-15.5%
Rat serum: 8.3-13.1%

Inter-assay (Run-to-Run)

Mouse plasma: 2.5-9.3%
Mouse serum: 5.6-10.2%
Rat plasma: 14.6-23.4%
Rat serum: 11.3-16.9%

Spiking Recovery

Mouse plasma: 86.2%
Mouse serum: 90.7%
Rat plasma: 101.9%
Rat serum: 97.9%
Mouse brain: 100.3 %
Rat brain: 88.7%

Summary

Research topic

Animal studies

Summary

Urocortin 3 (Ucn3) or stresscopin (SCP) is a new member of the corticotropin-releasing factor (CRF) peptide family
identified in the mouse and human. The CRF family of neuropeptides includes mammalian peptides CRF, urocortin
1(Ucn1) and urocortin 2 (Ucn2) or stress-related peptide (SRP), as well as piscine urotensin 1 and frog sauvagine.
Mouse and human Ucn3 share 90% identity in the 38-aa putative mature peptide.
In the human, Ucn1 immunoreactivity was marked in the medulla, whereas Ucn3 was immunostained mostly in the cortex. The receptors for Ucn1, Ucn2, Ucn3 and CRF are all expressed in human adrenal cortex and medulla, therefore these peptides are expected to play important roles in physiological adrenal functions. Ucn3 was also detected by RIA in human heart 0.74-1.15 pmol/g wet weight, kidney 1.21 pmol /g wet weight, pituitary 2.72 pmol /g wet weight and brain tissues 1-2 pmol /g wet weight. Furthermore, immunoreactive Ucn3 was present in human plasma 51.8 pmol/L and urine 266 pmol/L obtained from healthy subjects. It was also detected in human rectum 15.4 pmol/g wet weight and sigmoid colon 6.5 pmol/g wet weight. These data suggest that Ucn3 regulates the cardiac and renal functions as a local factor and a circulating hormone and plays some physiological or pathological roles in the modulation of gastrointestinal functions during stressful conditions in different manners from those of Ucn1. Pharmacological studies showed that Ucn3 is a high-affinity ligand for the type 2 CRF receptor (CRFR2). In the rat, Ucn3-positive neurons were found predominantly within the hypothalamus and medial amygdala. Ucn3 fibers were
distributed mainly in the hypothalamus and limbic structures. These data support that Ucn3 is an endogenous ligand
for CRFR2 in these areas. The results also suggest that Ucn3 is positioned to play a role in mediating physiological
functions, including food intake and neuroendocrine regulation.´n the mouse, Ucn3 was expressed in pancreatic beta-cells and in a mouse beta cell line, MIN6. High potassium, forskolin or high glucose could stimulate Ucn3 secretion from these cells. Ucn3 injections to the rat resulted in significant increase of plasma insulin level. Ucn3 also stimulated glucagon and insulin release from isolated rat islets. Pancreatic Ucn3 acting through CRFR2 was suggested to be involved in the local regulation of glucagon and insulin secretion. Treatment with Ucn3 (SCP) or Ucn2 (SRP) suppressed food intake, delayed gastric emptying and decreased heat-induced edema. Thus Ucn3 (SCP) and Ucn2 (SRP) might represent endogenous ligands for maintaining homeostasis after stress, and could allow the design of drugs to ameliorate stress-related diseases. The use of CRFR2 selective agonists, Ucn2 and Ucn3, to treat ischemic heart disease was proposed because of their potent cardioprotective effects in murine heart and their minimal impact on the hypothalamic stress axis. Ucn1 is able to bind to two types of G-protein coupled receptors: CRFR1 and CRFR2, whereas Ucn3 (SCP) and Ucn2 (SRP) bind exclusively and with high affinity to CRFR2. Ucn3 (SCP) is expressed in rat cardiomyocytes and the levels of Ucn3 (SCP) and Ucn2 (SRP) were increased by hypoxic stress. All these three peptide were shown to have potent cardioprotective effects in cells exposed to hypoxia/reoxygenation.

References to Product

References

  • Brar BK, Jonassen AK et al: (2004) Urocortin-II and urocortin-III are cardioprotective against ischemia reperfusion injury: an essential endogenous cardioprotective role for corticotropin releasing factor receptor type 2 in the murine heart. Endocrinology. 145, 24-35
  • Fukuta T, Takahashi K et al: (2005) Urocortin 1, urocortin3/stresscopin, and corticotropin- releasing factor receptors in human adrenal and its disorders. J Clin Endocrinol Metab. 90, 4671-467
  • Hsu SY and Hsueh AJ (2001) Human stresscopin and stresscopin-related peptide are selective ligands for the type 2 corticotropin-releasing hormone receptor. Nat Med. 7, 605-611
  • Chanalaris A, Lawrence KM et al: (2003) Protective effects of the urocortin homologues stresscopin (SCP) and stresscopin-related peptide (SRP) against hypoxia/reoxygenation injury in rat neonatal cardiomyocytes. J Mol Cell Cardiol. 35, 1295-1305
  • Lewis K, Li C et al: (2001) Identification of urocortin III, an additional member of the corticotropin-releasing factor (CRF) family with high affinity for the CRF2 receptor. Proc Natl Acad Sci USA. 98, 7570-7575
  • Li C, Vaughan J et al: (2002) Urocortin III-immunoreactive projections in rat brain: partial overlap with sites of type 2 corticotrophin-releasing factor receptor expression. J Neurosci. 22, 991-1001
  • Li C, Chen P et al: (2003) Urocortin III is expressed in pancreatic beta-cells and stimulates insulin and glucagon secretin. Endocrinology. 144, 3216-3224
  • Saruta M, Takahashi K et al: (2005) Urocortin3/stresscopin in human colon: possible modulators of gastrointestinal function during stressful conditions. Peptide. 26, 1196-1206
  • Takahashi K, Totsune K et al: (2004) Expression of urocortin III/stresscopin in human heart and kidney. J Clin Endocrinol Metab. 89, 1897-1903
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