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Uromodulin Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:Tamm-Horsfall urinary glycoprotein, THP, UMOD
  • Species:Human
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Cat. No. Size Price


RD191163200R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Urine

Sample Requirements

10 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

0.5–32 ng/ml

Limit of Detection

0.12 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 2.0%

Inter-assay (Run-to-Run)

n = 6; CV = 6.4%

Spiking Recovery

95,70%

Dilutation Linearity

99,90%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • monkey Yes
  • human Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 3.5 hours
  • The kit measures uromodulin in serum, plasma (EDTA, citrate, heparin) and urine
  • Assay format is 96 wells
  • Standard is native protein based
  • Quality Controls are human serum based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Renal disease

Summary

Uromodulin (Tamm-Horsfall protein, UMOD) is approx. 85-kDa glycoprotein that is produced in the thick ascending limb of Henle´s loop and early distal convoluted tubules of the nephron. It is a transmembrane protein, which is secreted into the urine through proteolytic cleavage of the glycosylphospha­tidylinositol (GPI) anchor. It belongs to the GPI family. Healthy individuals excrete tens of miligrams of uromodulin per day, making in the most abundant protein in the urine. Uromodulin modulates cell adhesion and signal transduction by interacting with cytokines and it inhibits the aggregation of calcium crystals. By reducing calcium oxalate precipitation, uromodulin plays a protective role with respect to renal stone formation as demonstrated by recent studies on THP- deficient mice prone to nephrolithiasis. THP acts as a host defense factor against urinary tract infections induced by uropathogens such as Esherichia coli, Staphylococcus saphrophyticus, Proteus mirabilis and Klebsiela pneumonie. Uromodulin binds to type 1 fimbriae of Escherichia coli and thereby blocks colonization of urothelial cells. Tamm-Horsfall protein interacts with other molecules and cells including IL-1, IL-2, TNF, IgG, neuthrophils, lymphocytes and monocytes. Binding of uromodulin to neutrophils induces synthesis of IL-8, provokes the respiratory burst and degranulation and stimulates chemotaxis and phagocytosis. Recently, genome-wide association studies identified uromodulin as a risk factor for chronic kidney disease and hypertension. Mutations in the Uromodulin gene are associated with three autosomal dominant tubulo-interstitial nephropathies such as familial juvenile hyperuricemic nephropathy (FJHN), medullary cystic kidney disease (MCKD2) and glomerulocystic kidney disease (GCKD). These disorders are characterized by juvenile onset of hyperuricemia, gout and progressive renal failure.

References to Product

References

  • Porrini E, Lima SL, Estupiñán S, Delgado P, Cobo M, Miquel R, Delgado AG, Mena NN, Marrero D, Perona AA, Rinne FG, Martín LD, Torres A; MP308 USING ESTIMATED GFR TO DIAGNOSE CKD STAGES: FLICKING THE COIN. Nephrol Dial Transplant 2017; 32 (suppl_3): iii539. doi: 10.1093/ndt/gfx168
  • Gast C, Marinaki T, Arenas-Hernandez M, Campbell S, Venkat-Raman G. Genetic testing reveals increased prevalence of uromodulin associated kidney disease. Nephrol Dial Transpl. 2015;30 (Supplement 3):iii55-iii56
  • Khasun M, Kayukov I, Beresneva O, Parastaeva M, Galkina O, Sipovskii V, Smirnov A; MP309 SERUM UROMODULIN IS EARLY BIOMARKER OF INTERSTITIAL FIBROSIS/TUBULAR ATROPHY IN PATIENTS WITH GLOMERLOPATHIES. Nephrol Dial Transplant 2017; 32 (suppl_3): iii539. doi: 10.1093/ndt/gfx168.MP309
  • Leiherer A, Muendlein A, Saely CH, Kinz E, Brandtner EM, Fraunberger P, Drexel H. Serum uromodulin is associated with impaired glucose metabolism. Medicine (Baltimore). 2017 Feb;96 (5):e5798
  • Leiherer A, Muendlein A, Saely CH, Ebner J, Brandtner EM, Fraunberger P, Drexel H. Data on the power of the creatinine to uromodulin ratio in serum to predict cardiovascular events in coronary patients. Data in Brief . March 2017; (11):576-580
  • Leiherer A, Muendlein A, Saely CH, Ebner J, Brandtner EM, Fraunberger P, Drexel H. Serum uromodulin is a predictive biomarker for cardiovascular events and overall mortality in coronary patients. Int J Cardiol. 2017 Mar 15;231:6-12. doi: 10.1016/j.ijcard.2016.12.183
  • Leiherer A, Muendlein A, Saely CH, Brandtner EM, Geiger K, Fraunberger P, Drexel H. The value of uromodulin as a new serum marker to predict decline in renal function. J Hypertens. 2017 Aug 30. doi: 10.1097/HJH.0000000000001527. PubMed PMID: 28858977.
  • Onoe T, Yamada K, Mizushima I, Ito K, Kawakami T, Daimon S, Muramoto H, Konoshita T, Yamagishi M, Kawano M. Hints to the diagnosis of uromodulin kidney disease. Clin Kidney J. 2016 Feb;9 (1):69-75
  • Risch L, Lhotta K, Meier D, Medina-Escobar P, Nydegger UE, Risch M. The serum uromodulin level is associated with kidney function. Clin Chem Lab Med. 2014 Jun 14;
  • Soukup V, Kalousova M, Capoun O, Sobotka R, Breyl Z, Pesl M, Zima T, Hanus T. Panel of Urinary Diagnostic Markers for Non-Invasive Detection of Primary and Recurrent Urothelial Urinary Bladder Carcinoma. Urol Int. 2015;95 (1):56-64
  • Botelho TE, Pereira AK, Teixeira PG, Lage EM, Osanan GC, Silva AC. Uromodulin: a new biomarker of fetal renal function? J Bras Nefrol. 2016 Dec;38(4):427-434. doi: 10.5935/0101-2800.20160068.
  • Chalupova L, Halupova L, Zakovska A, Krejci G, Svestak M, et al. (2016) CTRP1: A Molecular Link between Obesity and Hypertension . J Mol Biomark Diagn 7: 289. doi:10.4172/2155-9929.1000289
  • Fedak D, Kuźniewski M, Fugiel A, Wieczorek-Surdacka E, Przepiórkowska-Hoyer B, Jasik P, Miarka P, Dumnicka P, Kapusta M, Solnica B, Sułowicz W. Serum uromodulin concentrations correlate with glomerular filtration rate in patients with chronic kidney disease. Pol Arch Med Wewn. 2016 Dec 5;126(12):995-1004. doi: 10.20452/pamw.3712. Epub 2016 Dec 5.
  • Żyłka A, Dumnicka P, Kuśnierz-Cabala B, et al. Markers of Glomerular and Tubular Damage in the Early Stage of Kidney Disease in Type 2 Diabetic Patients. Mediators Inflamm. 2018;2018:7659243. Published 2018 Aug 9. doi:10.1155/2018/7659243
References to Summary

References to Uromodulin

  • Bleyer AJ, Zivna M, Kmoch S. Uromodulin-associated kidney disease. Nephron Clin Pract. 2011;118 (1):c31-6
  • Cavallone D, Malagolini N, Monti A, Wu XR, Serafini-Cessi F. Variation of high mannose chains of Tamm-Horsfall glycoprotein confers differential binding to type 1-fimbriated Escherichia coli. J Biol Chem. 2004 Jan 2;279 (1):216-22
  • Devuyst O, Dahan K, Pirson Y. Tamm-Horsfall protein or uromodulin: new ideas about an old molecule. Nephrol Dial Transplant. 2005 Jul;20 (7):1290-4
  • Gudbjartsson DF, Holm H, Indridason OS, Thorleifsson G, Edvardsson V, Sulem P, de Vegt F, d'Ancona FC, den Heijer M, Wetzels JF, Franzson L, Rafnar T, Kristjansson K, Bjornsdottir US, Eyjolfsson GI, Kiemeney LA, Kong A, Palsson R, Thorsteinsdottir U, Stefansson K. Association of variants at UMOD with chronic kidney disease and kidney stones-role of age and comorbid diseases. PLoS Genet. 2010 Jul;6 (7):e1001039
  • Kreft B, Jabs WJ, Laskay T, Klinger M, Solbach W, Kumar S, van Zandbergen G. Polarized expression of Tamm-Horsfall protein by renal tubular epithelial cells activates human granulocytes. Infect Immun. 2002 May;70 (5):2650-6
  • Lau WH, Leong WS, Ismail Z, Gam LH. Qualification and application of an ELISA for the determination of Tamm Horsfall protein (THP) in human urine and its use for screening of kidney stone disease. Int J Biol Sci. 2008;4 (4):215-22
  • Lhotta K. Uromodulin and chronic kidney disease. Kidney Blood Press Res. 2010;33 (5):393-8
  • Pourmand G, Nasseh H, Sarrafnejad A, Mehrsai A, Hamidi Alamdari D, Nourijelyani K, Shekarpour L. Urinary Tamm-Horsfall protein and citrate: a case-control study of inhibitors and promoters of calcium stone formation. Urol J. 2005 Spring;2 (2):79-85
  • Raffi HS, Bates JM Jr, Laszik Z, Kumar S. Tamm-horsfall protein protects against urinary tract infection by proteus mirabilis. J Urol. 2009 May;181 (5):2332-8
  • Rhodes DC. Binding of Tamm-Horsfall protein to complement 1q and complement 1, including influence of hydrogen-ion concentration. Immunol Cell Biol. 2002 Dec;80 (6):558-66
  • Zasloff M. Antimicrobial peptides, innate immunity, and the normally sterile urinary tract. J Am Soc Nephrol. 2007 Nov;18 (11):2810-6
  • Zaucke F, Boehnlein JM, Steffens S, Polishchuk RS, Rampoldi L, Fischer A, Pasch A, Boehm CW, Baasner A, Attanasio M, Hoppe B, Hopfer H, Beck BB, Sayer JA, Hildebrandt F, Wolf MT. Uromodulin is expressed in renal primary cilia and UMOD mutations result in decreased ciliary uromodulin expression. Hum Mol Genet. 2010 May 15;19 (10):1985-97
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