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Vaspin ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:Visceral adipose-specific serpin, Serpin A12, Visceral adipose tissue-derived serine protease inhibitor, OL-64, SERPINA12
  • Species:Human
Cat. No. Size Price

E106 96 wells (1 kit) $642
PubMed Product Details
Technical Data


Sandwich ELISA


The enzyme immunoassay for Vaspin E106 is a so-called Sandwich-Assay. It utilizes specific and high affinity polyclonal antibodies for this protein.



Sample Requirements

100 µl


Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.


Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

10-1000 pg/ml

Limit of Detection

4 pg/ml

Intra-assay (Within-Run)


Inter-assay (Run-to-Run)


Spiking Recovery




For Research Use Only
is suited for Vaspin determination in serum samples
is extremely sensitive (Limit of Blank 4 pg/ml)
is fast: incubation time a total of 3.5 hours
Single Standards with 10, 75, 200, 500,1000 pg/ml human Vaspin are provided in the kit
2 Control Sera are provided for quality control purposes according GLP
is calibrated with recombinant Vaspin
Microtiter plates are separately break apart, tests can be adapted to individual requirements

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction


The ELISA for quantitative measurement of Vaspin is based on polyclonal rabbit antisera raised by genetic immunization of the rabbits (cDNA Vaspin SC306941). Vaspin also known as SerpinA12 is a serine protease inhibitor and consists of 395 amino acids forming 3 β-sheets and 9 α-helices. Molecular weight of Vaspin is about 45.2 kDa. It does not form multimeric aggregates or intra-molecular disulfide bridges and no binding proteins in human serum are known. The Vaspin gene is not only expressed by subcutaneous and visceral adipose but also by liver tissue, in the pancreas1 and in the human epidermis (granular keratinocytes / GK cells)2.

Summary References (7)

References to Vaspin

  • Hida K, Wada J, Eguchi J, Zhang H, Baba M, Seida A, Hashimoto I, Okada T, Yasuhara A, Nakatsuka A, Shikata K, Hourai S, Futami J, Watanabe E, Matsuki Y, Hiramatsu R, Akagi S, Makino H, Kanwar YS. Visceral adipose tissue-derived serine protease inhibitor: a unique insulin-sensitizing adipocytokine in obesity. Proc Natl Acad Sci U S A. 2005 Jul 26;102 (30):10610-5
  • Kloting N, Berndt J, Kralisch S, Kovacs P, Fasshauer M, Schon MR, Stumvoll M, Bluher M. Vaspin gene expression in human adipose tissue: association with obesity and type 2 diabetes. Biochem Biophys Res Commun. 2006 Jan 6;339 (1):430-6
  • Li Q, Chen R, Moriya J, Yamakawa J, Sumino H, Kanda T, Takahashi T. A novel adipocytokine, visceral adipose tissue-derived serine protease inhibitor (vaspin), and obesity. J Int Med Res. 2008 Jul-Aug;36 (4):625-9
  • Seeger J, Ziegelmeier M, Bachmann A, Lossner U, Kratzsch J, Bluher M, Stumvoll M, Fasshauer M. Serum levels of the adipokine vaspin in relation to metabolic and renal parameters. J Clin Endocrinol Metab. 2008 Jan;93 (1):247-51
  • Tan BK, Heutling D, Chen J, Farhatullah S, Adya R, Keay SD, Kennedy CR, Lehnert H, Randeva HS. Metformin decreases the adipokine vaspin in overweight women with polycystic ovary syndrome concomitant with improvement in insulin sensitivity and a decrease in insulin resistance. Diabetes. 2008 Jun;57 (6):1501-7
  • Wada J. Vaspin: a novel serpin with insulin-sensitizing effects. Expert Opin Investig Drugs. 2008 Mar;17 (3):327-33
  • Youn BS, Kloting N, Kratzsch J, Lee N, Park JW, Song ES, Ruschke K, Oberbach A, Fasshauer M, Stumvoll M, Bluher M. Serum vaspin concentrations in human obesity and type 2 diabetes. Diabetes. 2008 Feb;57 (2):372-7
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