Type
Sandwich ELISA
Description
The enzyme immunoassay for Vaspin E106 is a so-called Sandwich-Assay. It utilizes specific and
high affinity polyclonal antibodies for this protein.
Applications
Serum
Sample Requirements
100 µl
Shipping
Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Range
10-1000 pg/ml
Limit of Detection
4 pg/ml
Intra-assay (Within-Run)
CV=2,69%
Inter-assay (Run-to-Run)
CV=5,94%
Spiking Recovery
97%
Features
For Research Use Only
is suited for Vaspin determination in serum samples
is extremely sensitive (Limit of Blank 4 pg/ml)
is fast: incubation time a total of 3.5 hours
Single Standards with 10, 75, 200, 500,1000 pg/ml human Vaspin are provided in the kit
2 Control Sera are provided for quality control purposes according GLP
is calibrated with recombinant Vaspin
Microtiter plates are separately break apart, tests can be adapted to individual requirements
Research topic
Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Reproduction
Summary
The ELISA for quantitative measurement of Vaspin is based on polyclonal rabbit antisera
raised by genetic immunization of the rabbits (cDNA Vaspin SC306941).
Vaspin also known as SerpinA12 is a serine protease inhibitor and consists of 395 amino
acids forming 3 β-sheets and 9 α-helices. Molecular weight of Vaspin is about 45.2 kDa. It
does not form multimeric aggregates or intra-molecular disulfide bridges and no binding
proteins in human serum are known. The Vaspin gene is not only expressed by
subcutaneous and visceral adipose but also by liver tissue, in the pancreas1 and in the
human epidermis (granular keratinocytes / GK cells)2.
Find documents for the lot
Example Instructions for Use (RUO)
Example Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
MSDS (RUO)
