Type
Sandwich ELISA, Biotin-labelled antibody
Applications
Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate, Cell culture supernatant
Sample Requirements
50 µl/well
Shipping
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).
Calibration Curve
Calibration Range
15.6–1000 pg/ml
Limit of Detection
7.9 pg/ml
Intra-assay (Within-Run)
CV = 6.2%
Inter-assay (Run-to-Run)
CV = 4.3%
Research topic
Cytokines and chemokines and related molecules, Oncology
Summary
VEGF is characterized by its highly specific mitogenic activity in endothelial cells and its angiogenic effect observed in vitro and in vivo. The VEGF family consists of four isoforms (A-D). VEGF-A is considered the most important of these with respect to tumor angiogenesis. and the splice variant isoforms VEGF121 and VEGF165 were found to be secreted by a wide spectrum of cell types, including smooth muscle cells, fibroblasts, epithelial cells, keratinocytes, macrophages, cardiac myocytes and various tumor cells. Inhibition of VEGF with a specific monoclonal antibody can suppress tumor growth in vivo.
Instructions for Use (RUO)
Instructions for Use (RUO)
Safety Information (RUO)
MSDS (RUO)
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