Western blotting, ELISA, Immunohistochemistry
Source of Antigen
293 cell line (Human embryonic kidney)
The antibody was raised in sheep by immunization with the recombinant Human ZA2G.
Amino Acid Sequence
The immunization antigen is a protein containing 290 AA of recombinant Human ZA2G. The AA sequence (AA 13–290) is identical to Swiss-Prot-P25311 (AA 18–295, mature Zinc-Alpha-2– Glycoprotein). 12 extra AA were fused with the N-terminus (highlighted).
Ala 1 to His 5 were confirmed by N-terminal sequencing
Human. Not yet tested in other species.
Immunoaffinity chromatography on a column with immobilized recombinant Human ZA2G.
0.1 mg (determined by BCA method, BSA was used as a standard)
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody BCA - to determine quantity of the antibody
This product is for research use only.