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Aldosterone Test Kit ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Other names:Aldocorten; Aldocortin; Electrocortin; Reichstein X; 18-Aldocorticosterone; 18-Oxocorticosterone; 11β,21-Dihydroxy-3,20-dioxopregn-4-en-18-al
  • Species:Human
Cat. No. Size Price

RCD030R 96 wells (1 kit) $413,06
PubMed Product Details
Technical Data


Competitive ELISA


Serum, Urine, Plasma

Sample Requirements

50 µL


At ambient temperature. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

15–1000 pg/ml

Limit of Detection

9.1 pg/ml

Intra-assay (Within-Run)

n = 4

CV = 7.7%

Inter-assay (Run-to-Run)

n = 5

CV = 10.8%

Spiking Recovery


Dilution Linearity




It is intended for research use only.

The kit measures aldosterone levels in serum, plasma and urine.

The total assay time is less than 2.5 hours.
Assay format is 96 wells

Standard is dried aldesterone in a protein-based buffer with a non-mercury preservative. It is prepared by spiking buffer with a defined quantity of aldosterone.

Research topic

Blood pressure regulation and NO metabolism, Bone and cartilage metabolism, Cardiovascular disease, Renal disease, Steroid hormones


Aldosterone is a potent mineralocorticoid whose synthesis and release are controlled by the renin-angiotensin system of the body. Aldosterone promotes the reabsorption of sodium in the distal tubules of the kidney resulting in potassium secretion along with sodium retention, which controls the circulating blood volume. Chronic overproduction and secretion of aldosterone leads to hypertension.

Product References (2)


  • Mori Y, Shimizu H, Kushima H, Hiromura M, Terasaki M, Tanaka M, Osaki A, Hirano T. Increased blood pressure in nesfatin/nuclebindin-2-transgenic mice. Hypertens Res. 2017 Oct 5;40(10):861-867. doi: 10.1038/hr.2017.61. Epub 2017 Apr 27. PubMed PMID: 28446807. See more on PubMed
  • Pollock M, Cameron S, Allpress S. Peripheral neuropathy in elderly CBA mice. Aust Paediatr J. 1988;24 Suppl 1:64-5. PubMed PMID: 2849396. See more on PubMed
Summary References (12)

References to Aldosterone

  • Al-Dujaili EA, Edwards CR. Optimization of a direct radioimmunoassay for plasma aldosterone. J Steroid Biochem. 1981 May;14 (5):481-7
  • Cartledge S, Lawson N. Aldosterone and renin measurements. Ann Clin Biochem. 2000 May;37 ( Pt 3):262-78
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Corry DB, Tuck ML. Secondary aldosteronism. Endocrinol Metab Clin North Am. 1995 Sep;24 (3):511-29
  • Himathongkam T, Dluhy RG, Williams GH. Potassim-aldosterone-renin interrelationships. J Clin Endocrinol Metab. 1975 Jul;41 (1):153-9
  • Lun S, Espiner EA, Nicholls MG, Yandle TG. A direct radioimmunoassay for aldosterone in plasma. Clin Chem. 1983 Feb;29 (2):268-71
  • Miller MA, Sagnella GA, MacGregor GA. Extraction method and nonextracted kit method compared for measuring plasma aldosterone. Clin Chem. 1997 Oct;43 (10):1995-7
  • Oelkers W, Diederich S, Bahr V. Diagnosis and therapy surveillance in Addison's disease: rapid adrenocorticotropin (ACTH) test and measurement of plasma ACTH, renin activity, and aldosterone. J Clin Endocrinol Metab. 1992 Jul;75 (1):259-64
  • Sequeira SJ, Loughlin T, Cunningham S, Culliton MT, Hannon S, Heffernan A, McKenna TJ. Evaluation of an aldosterone radioimmunoassay: the renin-angiotensin-aldosterone axis as a function of sex and age. Ann Clin Biochem. 1986 Jan;23 ( Pt 1):65-75
  • Stabler TV, Siegel AL. Chemiluminescence immunoassay of aldosterone in serum. Clin Chem. 1991 Nov;37 (11):1987-9
  • Vallotton MB. Primary aldosteronism. Part I. Diagnosis of primary hyperaldosteronism. Clin Endocrinol (Oxf). 1996 Jul;45 (1):47-52
  • Varsano-Aharon N, Ulick S. Further simplifications in the immunoassay of plasma aldosterone. J Clin Endocrinol Metab. 1974 Aug;39 (2):375-9
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