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ALDOSTERONE ELISA

  • Regulatory status:RUO
  • Type:Competitive ELISA
  • Species:Human
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New DKO053 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Competitive ELISA

Applications

Serum, Urine, Plasma

Shipping

Shipped on ice packs. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Range

20 - 2000 pg/ml

Limit of Detection

15 pg/ml

Summary

Features

European Union: for in vitro diagnostic use

Rest of the world: for research use only!

The total assay time is less than 2.5 hours

Quantitative determination of aldosterone in human serum, plasma and urine

No cross-reactivity with the other steroid hormones

Assay format is 96 wells

Quality Controls (A and B) are ready to use

Standards are ready to use

For routine analysis

Research topic

Blood pressure regulation and NO metabolism, Cardiovascular disease, Renal disease, Steroid hormones

Summary

Aldosterone is a steroid hormone produced by the adrenal cortex in the adrenal gland, is the most potent mineralocorticoid in humans, it regulate sodium and potassium balance in the blood. Aldosterone secretion appears to be stimulated primarily through the renin-angiotensin system. Acting on mineralocorticoid receptors (MR) on principal cells in the collecting ducts of the kidneys, it increases the permeability of their apical (luminal) membrane to potassium and sodium and activates their basolateral Na+/K+ pumps, stimulating ATP hydrolysis, reabsorbing sodium (Na+) ions and water into the blood, and excreting potassium (K+) ions into the urine. Aldosterone regulate plasma bicarbonate (HCO3-) levels and its acid/base balance. Aldosterone is responsible for the reabsorption of about 2% of filtered sodium in the kidneys. Plasma aldosterone levels normally vary with body position (upright>supine) and salt intake. Overall plasma aldosterone levels show a circadian rhythm which is similar to but less marked than cortisol, with peak levels in the early morning; about 75% of the daily production is secreted between 04:00 am and 10:00 am each day. Age-related levels tend to decline from fetal through adult life. Abnormally high plasma aldosterone concentrations can occur in adenomas, glucocorticoid-responsive hyperaldosteronism, idiopathic. Abnormally low aldosterone secretion occurs in a number of conditions including salt-wasting forms of congenital adrenal hyperplasia, nephropathy, and renal tubular acidosis.

Summary References (12)

References to Aldosterone

  • Al-Dujaili EA, Edwards CR. Optimization of a direct radioimmunoassay for plasma aldosterone. J Steroid Biochem. 1981 May;14 (5):481-7
  • Cartledge S, Lawson N. Aldosterone and renin measurements. Ann Clin Biochem. 2000 May;37 ( Pt 3):262-78
  • Check JH, Ubelacker L, Lauer CC. Falsely elevated steroidal assay levels related to heterophile antibodies against various animal species. Gynecol Obstet Invest. 1995;40 (2):139-40
  • Corry DB, Tuck ML. Secondary aldosteronism. Endocrinol Metab Clin North Am. 1995 Sep;24 (3):511-29
  • Himathongkam T, Dluhy RG, Williams GH. Potassim-aldosterone-renin interrelationships. J Clin Endocrinol Metab. 1975 Jul;41 (1):153-9
  • Lun S, Espiner EA, Nicholls MG, Yandle TG. A direct radioimmunoassay for aldosterone in plasma. Clin Chem. 1983 Feb;29 (2):268-71
  • Miller MA, Sagnella GA, MacGregor GA. Extraction method and nonextracted kit method compared for measuring plasma aldosterone. Clin Chem. 1997 Oct;43 (10):1995-7
  • Oelkers W, Diederich S, Bahr V. Diagnosis and therapy surveillance in Addison's disease: rapid adrenocorticotropin (ACTH) test and measurement of plasma ACTH, renin activity, and aldosterone. J Clin Endocrinol Metab. 1992 Jul;75 (1):259-64
  • Sequeira SJ, Loughlin T, Cunningham S, Culliton MT, Hannon S, Heffernan A, McKenna TJ. Evaluation of an aldosterone radioimmunoassay: the renin-angiotensin-aldosterone axis as a function of sex and age. Ann Clin Biochem. 1986 Jan;23 ( Pt 1):65-75
  • Stabler TV, Siegel AL. Chemiluminescence immunoassay of aldosterone in serum. Clin Chem. 1991 Nov;37 (11):1987-9
  • Vallotton MB. Primary aldosteronism. Part I. Diagnosis of primary hyperaldosteronism. Clin Endocrinol (Oxf). 1996 Jul;45 (1):47-52
  • Varsano-Aharon N, Ulick S. Further simplifications in the immunoassay of plasma aldosterone. J Clin Endocrinol Metab. 1974 Aug;39 (2):375-9
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