Western blotting, ELISA, Dot, IS
WB (with ECL): 1:1000–10,000 dilution (incubate for 1 hour at room temperature) IS: 1:500–2000 dilution For best results with other assays (e.g.: Dot, ELISA, etc), please determine optimal working dilution by titration test
Protein A affinity chromatography from mouse ascites fluid
1 mg/mL in 10 mM PBS, pH 7.2, with 0.05% NaN3
Store at –20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1–2 weeks
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme commonly known to be involved in glycolysis. The enzyme exists as a tetramer of identical 37 kDa subunits. GAPDH catalyzes the reversible reduction of 1,3– bisphos-phoglycerate to glyceraldehyde 3– phosphophate in the presence of NADPH. Apart from playing a key role in glycolysis, this ubiquitously expressed enzyme also displays other activities unrelated to its glycolytic function. GAPDH is reported to be involved in the processes of DNA replication, DNA repair, nuclear RNA export, membrane fusion and microtubule bundling. Other studies also provide evidence of GAPDH playing an essential part of the program of gene expression observed in apoptosis and as part of the cellular phenotype of age-related neurodegenerative diseases.