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Anti-IgA isotype IgG Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, HRP-labelled antibody
  • Species:Human
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Cat. No. Size Price


RD199145100R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, HRP-labelled antibody

Applications

Serum, Plasma

Sample Requirements

5 μl/well

Storage/Expiration

Store the complete kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

1.56–100 U/ml

Limit of Detection

0.71 U/ml

Intra-assay (Within-Run)

n = 8; CV = 4.8%

Inter-assay (Run-to-Run)

n = 5; CV = 5.3%

Spiking Recovery

102,00%

Dilutation Linearity

91,00%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 3 hours.
  • The kit measures IgG anti-IgA antibodies in serum and plasma (EDTA, citrate, heparin).
  • Assay format is 96 wells.
  • Calibrator is serum based.
  • Quality Controls are human serum based. No animal sera are used.
  • Components of the kit are provided ready to use, concentrated or lyophilized.

Research topic

Autoimmunity, Immune Response, Infection and Inflammation, Immunology, Transplantation

Summary

Anti-IgA in the IgG class are specific IgG antibodies against immunoglobulin A. Patients with IgA deficiency often demonstrate circulating antibodies against IgA, which have been suggested to be associated with transfusion reactions. Anti-IgA antibodies occur in about 10% of patients with selective IgA deficiency (IgAD) and are detected approximately in the same percentage of patients with Common Variable Immunodeficiency (CVID). Patients with anti-IgA antibodies are at risk to develop an anaphylactoid reaction to substitution gamma globulin treatment, especially upon intravenous administration.

References to Product

References

  • Chovancova Z, Vlkova M, Litzman J, Lokaj J, Thon V. Antibody forming cells and plasmablasts in peripheral blood in CVID patients after vaccination. Vaccine. 2011 Apr 5;
  • Thon, V. Substituční imunoglobulinová léčba. In Miloš Jeseňák, Peter Bánovčin. Vrodené poruchy imunity. A - medi management. 2014;Bratislava:503 -517
  • Torabi Sagvand B, Mirminachi B, Abolhassani H, Shokouhfar T, Keihanian T, Amirzargar A, Mahdaviani A, Aghamohammadi A. IgG anti-IgA antibodies in paediatric antibody-deficient patients receiving intravenous immunoglobulin. Allergol Immunopathol (Madr). 2014 Sep 5;
References to Summary

References to Anti-IgA IgG

  • Davenport RD, Mintz PD. Transfusion medicine. In Henry's Clinical Diagnosis. 2006;:707-708
  • de Albuquerque Campos R, Sato MN, da Silva Duarte AJ. IgG anti-IgA subclasses in common variable immunodeficiency and association with severe adverse reactions to intravenous immunoglobulin therapy. J Clin Immunol. 2000 Jan;20 (1):77-82
  • Hammarstrom L, Persson MA, Smith CI. Anti-IgA in selective IgA deficiency. In vitro effects and Ig subclass pattern of human anti-IgA. Scand J Immunol. 1983 Dec;18 (6):509-13
  • Horn J, Thon V, Bartonkova D, Salzer U, Warnatz K, Schlesier M, Peter HH, Grimbacher B. Anti-IgA antibodies in common variable immunodeficiency (CVID): diagnostic workup and therapeutic strategy. Clin Immunol. 2007 Feb;122 (2):156-62
  • Koskinen S, Hirvonen M, Tolo H. An enzyme immunoassay for the determination of anti-IgA antibodies using polyclonal human IgA. J Immunol Methods. 1995 Feb 13;179 (1):51-8
  • Limaye S, Walls RS, Riminton S. Safe and effective use of chromatographically purified intravenous immunoglobulin despite profound anti-IgA sensitization. Intern Med J. 2001 May-Jun;31 (4):256-7
  • Litzman J, Sevcikova I, Stikarovska D, Pikulova Z, Pazdirkova A, Lokaj J. IgA deficiency in Czech healthy individuals and selected patient groups. Int Arch Allergy Immunol. 2000 Oct;123 (2):177-80
  • Quartier P. [IgA deficiency]. Arch Pediatr. 2001 Jun;8 (6):629-33
  • Salama A, Schwind P, Schonhage K, Genth R, Cotting C, Hustinx H, Krieg R, Nydegger U, Aebischer I. Rapid detection of antibodies to immunoglobulin A molecules by using the particle gel immunoassay. Vox Sang. 2001 Jul;81 (1):45-8
  • Sandler SG, Eckrich R, Malamut D, Mallory D. Hemagglutination assays for the diagnosis and prevention of IgA anaphylactic transfusion reactions. Blood. 1994 Sep 15;84 (6):2031-5
  • Sandler SG, Zantek ND. Review: IgA anaphylactic transfusion reactions. Part II. Clinical diagnosis and bedside management. Immunohematology. 2004;20 (4):234-8
  • Sanz C, Freire C, Ordinas A, Pereira A. An enzyme-linked immunosorbent assay applicable to screen blood donors for IgA deficiency. Haematologica. 1999 Oct;84 (10):887-90
  • Steensma DP, Pruthi RK. Hematology. In Mayo Clinic Internal Medici. 2008;:427
  • Sundin U, Nava S, Hammarstrom L. Induction of unresponsiveness against IgA in IgA-deficient patients on subcutaneous immunoglobulin infusion therapy. Clin Exp Immunol. 1998 May;112 (2):341-6
  • Vassallo RR. Review: IgA anaphylactic transfusion reactions. Part I. Laboratory diagnosis, incidence, and supply of IgA-deficient products. Immunohematology. 2004;20 (4):226-33
  • Vorechovsky I, Webster AD, Plebani A, Hammarstrom L. Genetic linkage of IgA deficiency to the major histocompatibility complex: evidence for allele segregation distortion, parent-of-origin penetrance differences, and the role of anti-IgA antibodies in disease predisposition. Am J Hum Genet. 1999 Apr;64 (4):1096-109
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