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Manufactured by BioVendor

Canine Uromodulin ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA
  • Other names:Tamm-Horsfall urinary glycoprotein, THP, UMOD
  • Species:Canine
Cat. No. Size Price

New RBL023R 96 wells (1 kit) $526,55
PubMed Product Details
Technical Data


Sandwich ELISA


Serum, Urine, Plasma

Sample Requirements



Shipped on blue ice packs. Upon receipt, store the product at the temperature recommended below.


Store the complete kit at 2–8°C. Under these conditions, all components are stable until the expiration date (see label on the box).

Calibration Range

0,16 - 10 ng/ml

Limit of Detection

10 pg/ml

Intra-assay (Within-Run)

n = 8 CV = 3.9%

Inter-assay (Run-to-Run)

n = 8, CV = 7%

Spiking Recovery


Dilution Linearity




It is intended for research use only The total assay time is less than 3 hours The kit measures canine uromodulin Assay format is 96 wells Standard is a recombinant protein Components of the kit are provided ready to use, concentrated or dried

Research topic

Renal disease


It has been suggested that uromodulin protein may be an important component of epithelia, which absorb sodium and chloride ions but are impermeable to water. Uromodulin interacts with other molecules and cells including IL-1, IL-2, TNF, IgG, neuthrophils, lymphocytes and monocytes. Uromodulin also acts as a host defense factor against urinary tract infections, specifically targeting type 1 fimbriated Escherichia coli. Uromodulin also acts as an inhibitor of stone formation in healthy individuals by trapping crystals in the same manner. However, this function may be subverted under some circumstances and uromodulin may facilitate crystal aggregation and then promote stone formation. It means that it has been proposed to play a dual role as promoter or inhibitor of nephrolithiasis. Previous reports have described urinary uromodulin as biomarker for distal tubular dysfunction in dogs. Its potential use is illustrated by decreased urinary uromodulin in dogs with chronic kidney diseases (CKD) and in dogs with urolithiasis. The high metabolic activity of the cells of the thick ascending limb of Henle’s loop makes them particularly vulnerable to ischemic injury. Consequently, in acute kidney injury (AKI) caused by ischemic insults, evaluation of uromodulin as an early AKI biomarker is potentially valuable. The study on the Bernese mountain dogs concluded that uromodulin together with the other identified urinary proteins may be valuable markers for the diagnosis of juvenile nephropathy.

Summary References (12)

References to Uromodulin

  • Bleyer AJ, Zivna M, Kmoch S. Uromodulin-associated kidney disease. Nephron Clin Pract. 2011;118 (1):c31-6
  • Cavallone D, Malagolini N, Monti A, Wu XR, Serafini-Cessi F. Variation of high mannose chains of Tamm-Horsfall glycoprotein confers differential binding to type 1-fimbriated Escherichia coli. J Biol Chem. 2004 Jan 2;279 (1):216-22
  • Devuyst O, Dahan K, Pirson Y. Tamm-Horsfall protein or uromodulin: new ideas about an old molecule. Nephrol Dial Transplant. 2005 Jul;20 (7):1290-4
  • Gudbjartsson DF, Holm H, Indridason OS, Thorleifsson G, Edvardsson V, Sulem P, de Vegt F, d'Ancona FC, den Heijer M, Wetzels JF, Franzson L, Rafnar T, Kristjansson K, Bjornsdottir US, Eyjolfsson GI, Kiemeney LA, Kong A, Palsson R, Thorsteinsdottir U, Stefansson K. Association of variants at UMOD with chronic kidney disease and kidney stones-role of age and comorbid diseases. PLoS Genet. 2010 Jul;6 (7):e1001039
  • Kreft B, Jabs WJ, Laskay T, Klinger M, Solbach W, Kumar S, van Zandbergen G. Polarized expression of Tamm-Horsfall protein by renal tubular epithelial cells activates human granulocytes. Infect Immun. 2002 May;70 (5):2650-6
  • Lau WH, Leong WS, Ismail Z, Gam LH. Qualification and application of an ELISA for the determination of Tamm Horsfall protein (THP) in human urine and its use for screening of kidney stone disease. Int J Biol Sci. 2008;4 (4):215-22
  • Lhotta K. Uromodulin and chronic kidney disease. Kidney Blood Press Res. 2010;33 (5):393-8
  • Pourmand G, Nasseh H, Sarrafnejad A, Mehrsai A, Hamidi Alamdari D, Nourijelyani K, Shekarpour L. Urinary Tamm-Horsfall protein and citrate: a case-control study of inhibitors and promoters of calcium stone formation. Urol J. 2005 Spring;2 (2):79-85
  • Raffi HS, Bates JM Jr, Laszik Z, Kumar S. Tamm-horsfall protein protects against urinary tract infection by proteus mirabilis. J Urol. 2009 May;181 (5):2332-8
  • Rhodes DC. Binding of Tamm-Horsfall protein to complement 1q and complement 1, including influence of hydrogen-ion concentration. Immunol Cell Biol. 2002 Dec;80 (6):558-66
  • Zasloff M. Antimicrobial peptides, innate immunity, and the normally sterile urinary tract. J Am Soc Nephrol. 2007 Nov;18 (11):2810-6
  • Zaucke F, Boehnlein JM, Steffens S, Polishchuk RS, Rampoldi L, Fischer A, Pasch A, Boehm CW, Baasner A, Attanasio M, Hoppe B, Hopfer H, Beck BB, Sayer JA, Hildebrandt F, Wolf MT. Uromodulin is expressed in renal primary cilia and UMOD mutations result in decreased ciliary uromodulin expression. Hum Mol Genet. 2010 May 15;19 (10):1985-97
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