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Cartilage Oligomeric Matrix Protein Human ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, Biotin-labelled antibody
  • Other names:COMP, Thrombospondin-5, TSP5
  • Species:Human
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Cat. No. Size Price


RD194080200 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, Biotin-labelled antibody

Applications

Serum, Plasma-EDTA, Plasma-Heparin, Plasma-Citrate

Sample Requirements

5 µl/well

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

4–128 ng/ml

Limit of Detection

0.4 ng/ml

Intra-assay (Within-Run)

n = 8; CV = 6.0%

Inter-assay (Run-to-Run)

n = 8; CV = 4.9%

Spiking Recovery

97,60%

Dilution Linearity

95,80%

Crossreactivity

  • bovine Non-detectable
  • cat Non-detectable
  • dog Non-detectable
  • goat Non-detectable
  • hamster Non-detectable
  • horse Non-detectable
  • monkey Non-detectable
  • mouse Non-detectable
  • pig Non-detectable
  • rabbit Non-detectable
  • rat Non-detectable
  • sheep Non-detectable
  • chicken Not tested
  • human Yes
Summary

Features

  • European Union: for in vitro diagnostic use
  • Rest of the world: for research use only!
  • The total assay time is less than 3.5 hours
  • The kit measures COMP in serum and plasma (EDTA, citrate, heparin)
  • Assay format is 96 wells
  • Quality Controls are human serum based
  • Standard is recombinant protein based
  • Components of the kit are provided ready to use, concentrated or lyophilized

Research topic

Atherosclerosis, Bone and cartilage metabolism, Cardiovascular disease, Extracellular matrix, Pulmonary diseases

Summary

Cartilage oligomeric matrix protein (COMP), also designated thrombospondin 5 (TSP 5), is a non-collagenous glycoprotein and is a member of the thrombospondin family of extracellular proteins. COMP is a calcium-binding protein of high molecular weight (>500kDa) present in the extracellular matrix of articular, nasal and tracheal cartilage. COMP is not only cartilage-derived but was found widely in other tissues, including synovium and tendon. Intact COMP is pentameric, with five identical subunits and the carboxy-terminal globular domain of native COMP binds to collagens I, II, and IX. It has been proposed that COMP molecules are important for maintaining the properties and integrity of collagen network. In addition COMP may have a storage and delivery function for hydrophobic cell-signaling molecules such as vitamin D.

The significance of COMP for normal development and function of cartilage has been underscored by the discovery that mutations of the COMP gene result in pseudoachondro¬plasia and some forms of multiple epiphyseal dysplasia. Most published studies have shown that serum levels of COMP provide important information about metabolic changes occurring in the cartilage matrix in joint disease. These studies describe that serum COMP level correlated with cartilage degradation and is a potential prognostic marker in inflammatory joint diseases such as osteoarthritis (OA) and rheumatoid arthritis (RA). Results have demonstrated an association of increasing serum COMP levels with progressive destruction of articular cartilage monitored radiographically. OA and RA are common diseases causing pain and disability in a significant proportion of the adult population and early diagnostics of these diseases is very important for future therapy.

COMP, abundant in cartilage, is now known to be also expressed in tumor tissues from breast, prostate, and colon cancer. Studies have concluded that COMP in breast cancer cells is correlated with poor survival and higher recurrence rates in patients. Elevated levels of COMP in sera can be detected in metastatic breast cancer patients compared with those in early stages of the disease. Additionally, serum COMP could potentially be an independent prognostic marker for metastatic patients. Similarly, studies have shown that COMP expression in prostate cancer correlated with enhanced invasion and with a more progressive disease. Likewise, COMP expression has been correlated with the poor outcome of colon cancer patients. COMP levels in the sera of preoperative patients with colon cancer were much higher than those in healthy donors and were significantly reduced after colectomy. Thus, COMP may be a novel prognostic indicator and biomarker and also a potential therapeutic target for colon cancer.

Increased COMP has been associated with fibrogenesis in systemic sclerosis, skin keloids, vascular atherosclerosis, lung fibrosis, and chronic pancreatitis. Studies have also suggested that COMP contributes to liver fibrosis by regulating collagen-I deposition, with further investigation finding that serum COMP levels could be used as a non-invasive liver fibrosis marker in patients with chronic viral hepatitis (CVH). There is evidence that COMP plays a role in pathogenesis of idiopathic pulmonary fibrosis (IPF) and should be further evaluated as a biomarker for disease activity in IPF.

There is evidence that COMP is essential for maintaining vascular homeostasis, and is an endogenous thrombin inhibitor and negative regulator of hemostasis and thrombosis. Research suggests a pathogenetic role of COMP the development of vascular fibrosis and calcification, and thus COMP may be a novel target in therapy of a number of vascular diseases.

Product References (17)

References

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  • Dragomir AD, Kraus VB, Renner JB, Luta G, Clark A, Vilim V, Hochberg MC,Helmick CG, Jordan JM. Serum cartilage oligomeric matrix protein and clinicalsigns and symptoms of potential pre-radiographic hip and knee pathology.Osteoarthritis Cartilage. 2002 Sep;10(9):687-91. PubMed PMID: 12202121. See more on PubMed
  • Henrotin Y, Bannuru R, Malaise M, Ea HK, Confavreux C, Bentin J,Urbin-Choffray D, Conrozier T, Brasseur JP, Thomas P, Hick AC, Marinello A,Giordan N, Richette P. Hyaluronan derivative HYMOVIS® increases cartilage volume and type ii collagen turnover in osteoarhritic knee: data from MOKHA study. BMCMusculoskelet Disord. 2019 Jun 18;20(1):293. doi: 10.1186/s12891-019-2667-0.PubMed PMID: 31215422; PubMed Central PMCID: PMC6580647. See more on PubMed
  • Huang X, Post JN, Zhong L, Leijten J, Larsson S, Karperien M, Struglics A.Dickkopf-related protein 1 and gremlin 1 show different response thanfrizzled-related protein in human synovial fluid following knee injury and inpatients with osteoarthritis. Osteoarthritis Cartilage. 2018 Jun;26(6):834-843.doi: 10.1016/j.joca.2018.02.904. Epub 2018 Mar 8. PubMed PMID: 29526783. See more on PubMed
  • Chandran V, Cook RJ, Edwin J, Shen H, Pellett FJ, Shanmugarajah S, Rosen CF,Gladman DD. Soluble biomarkers differentiate patients with psoriatic arthritisfrom those with psoriasis without arthritis. Rheumatology (Oxford). 2010Jul;49(7):1399-405. doi: 10.1093/rheumatology/keq105. Epub 2010 Apr 25. PubMedPMID: 20421218. See more on PubMed
  • Jordan JM, Luta G, Stabler T, Renner JB, Dragomir AD, Vilim V, Hochberg MC,Helmick CG, Kraus VB. Ethnic and sex differences in serum levels of cartilageoligomeric matrix protein: the Johnston County Osteoarthritis Project. Arthritis Rheum. 2003 Mar;48(3):675-81. PubMed PMID: 12632420. See more on PubMed
  • Kopec-Medrek M, Kucharz EJ. Fibulin-3 and other cartilage metabolismbiomarkers in relationship to calprotectin (MRP8/14) and disease activity inrheumatoid arthritis patients treated with anti-TNF therapy. Adv Clin Exp Med.2018 Mar;27(3):383-389. doi: 10.17219/acem/68362. PubMed PMID: 29533545. See more on PubMed
  • Lattermann C, Jacobs CA, Bunnell MP, Jochimsen KN, Abt JP, Reinke EK, GammonLG, Huebner JL, Kraus VB, Spindler KP. Logistical challenges and designconsiderations for studies using acute anterior cruciate ligament injury as apotential model for early posttraumatic osteoarthritis. J Orthop Res. 2017Mar;35(3):641-650. doi: 10.1002/jor.23329. Epub 2016 Jun 30. PubMed PMID:27279368. See more on PubMed
  • Liu B, Goode AP, Carter TE, Utturkar GM, Huebner JL, Taylor DC, Moorman CT3rd, Garrett WE, Kraus VB, Guilak F, DeFrate LE, McNulty AL. Matrixmetalloproteinase activity and prostaglandin E2 are elevated in the synovialfluid of meniscus tear patients. Connect Tissue Res. 2017 May -Jul;58(3-4):305-316. doi: 10.1080/03008207.2016.1256391. Epub 2016 Nov 4. PubMed PMID: 27813662; PubMed Central PMCID: PMC5418110. See more on PubMed
  • Misumi K, Vilim V, Clegg PD, Thompson CC, Carter SD. Measurement of cartilage oligomeric matrix protein (COMP) in normal and diseased equine synovial fluids.Osteoarthritis Cartilage. 2001 Feb;9(2):119-27. PubMed PMID: 11237659. See more on PubMed
  • Riegger J, Rehm M, Büchele G, Brenner H, Günther KP, Rothenbacher D, BrennerRE. Serum Cartilage Oligomeric Matrix Protein in Late-Stage Osteoarthritis:Association with Clinical Features, Renal Function, and CardiovascularBiomarkers. J Clin Med. 2020 Jan 18;9(1). pii: E268. doi: 10.3390/jcm9010268.PubMed PMID: 31963737. See more on PubMed
  • Stidham RW, Wu J, Shi J, Lubman DM, Higgins PD. Serum Glycoproteome Profilesfor Distinguishing Intestinal Fibrosis from Inflammation in Crohn's Disease. PLoSOne. 2017 Jan 23;12(1):e0170506. doi: 10.1371/journal.pone.0170506. eCollection2017. PubMed PMID: 28114331; PubMed Central PMCID: PMC5256928. See more on PubMed
  • Swärd P, Struglics A, Englund M, Roos HP, Frobell RB. Soft tissue knee injury with concomitant osteochondral fracture is associated with higher degree of acutejoint inflammation. Am J Sports Med. 2014 May;42(5):1096-102. doi:10.1177/0363546514524924. Epub 2014 Mar 24. PubMed PMID: 24664137. See more on PubMed
  • Vilim V, Lenz ME, Vytasek R, Masuda K, Pavelka K, Kuettner KE, Thonar EJ.Characterization of monoclonal antibodies recognizing different fragments ofcartilage oligomeric matrix protein in human body fluids. Arch Biochem Biophys.1997 May 1;341(1):8-16. PubMed PMID: 9143347. See more on PubMed
  • Vilím V, Olejárová M, Machácek S, Gatterová J, Kraus VB, Pavelka K. Serumlevels of cartilage oligomeric matrix protein (COMP) correlate with radiographic progression of knee osteoarthritis. Osteoarthritis Cartilage. 2002Sep;10(9):707-13. PubMed PMID: 12202123. See more on PubMed
  • Vilím V, Vobůrka Z, Vytásek R, Senolt L, Tchetverikov I, Kraus VB, Pavelka K. Monoclonal antibodies to human cartilage oligomeric matrix protein: epitopemapping and characterization of sandwich ELISA. Clin Chim Acta. 2003Feb;328(1-2):59-69. PubMed PMID: 12559599. See more on PubMed
  • Wang SH, Shen CY, Weng TC, Lin PH, Yang JJ, Chen IF, Kuo SM, Chang SJ, Tu YK, Kao YH, Hung CH. Detection of cartilage oligomeric matrix protein using a quartz crystal microbalance. Sensors (Basel). 2010;10(12):11633-43. doi:10.3390/s101211633. Epub 2010 Dec 20. PubMed PMID: 22163547; PubMed CentralPMCID: PMC3231063. See more on PubMed
Summary References (23)

References to Cartilage Oligomeric Matrix Protein

  • Agarwal P, Schulz JN, Blumbach K, Andreasson K, Heinegård D, Paulsson M, Mauch C, Eming SA, Eckes B, Krieg T. Enhanced deposition of cartilage oligomeric matrix protein is a common feature in fibrotic skin pathologies. Matrix Biol. 2013 Aug 8;32(6):325-31. doi: 10.1016/j.matbio.2013.02.010. Epub 2013 Mar 15. PMID: 23507196. See more on PubMed
  • Bruyere O, Collette JH, Ethgen O, Rovati LC, Giacovelli G, Henrotin YE, SeidelL, Reginster JY. Biochemical markers of bone and cartilage remodeling inprediction of longterm progression of knee osteoarthritis. J Rheumatol. 2003May;30(5):1043-50. PubMed PMID: 12734904. See more on PubMed
  • Canfield AE, Farrington C, Dziobon MD, Boot-Handford RP, Heagerty AM, Kumar SN, Roberts IS. The involvement of matrix glycoproteins in vascular calcification and fibrosis: an immunohistochemical study. J Pathol. 2002 Feb;196(2):228-34. doi: 10.1002/path.1020. PMID: 11793375. See more on PubMed
  • Dragomir AD, Kraus VB, Renner JB, Luta G, Clark A, Vilim V, Hochberg MC,Helmick CG, Jordan JM. Serum cartilage oligomeric matrix protein and clinicalsigns and symptoms of potential pre-radiographic hip and knee pathology.Osteoarthritis Cartilage. 2002 Sep;10(9):687-91. PubMed PMID: 12202121. See more on PubMed
  • Englund E, Bartoschek M, Reitsma B, Jacobsson L, Escudero-Esparza A, Orimo A, Leandersson K, Hagerling C, Aspberg A, Storm P, Okroj M, Mulder H, Jirström K, Pietras K, Blom AM. Cartilage oligomeric matrix protein contributes to the development and metastasis of breast cancer. Oncogene. 2016 Oct 27;35(43):5585-5596. doi: 10.1038/onc.2016.98. Epub 2016 Apr 11. PMID: 27065333. See more on PubMed
  • Englund E, Canesin G, Papadakos KS, Vishnu N, Persson E, Reitsma B, Anand A, Jacobsson L, Helczynski L, Mulder H, Bjartell A, Blom AM. Cartilage oligomeric matrix protein promotes prostate cancer progression by enhancing invasion and disrupting intracellular calcium homeostasis. Oncotarget. 2017 Sep 21;8(58):98298-98311. doi: 10.18632/oncotarget.21176. PMID: 29228690; PMCID: PMC5716730. See more on PubMed
  • Farina G, Lemaire R, Korn JH, Widom RL. Cartilage oligomeric matrix protein is overexpressed by scleroderma dermal fibroblasts. Matrix Biol. 2006 May;25(4):213-22. doi: 10.1016/j.matbio.2006.01.007. Epub 2006 Mar 7. PMID: 16520029. See more on PubMed
  • Inui S, Shono F, Nakajima T, Hosokawa K, Itami S. Identification and characterization of cartilage oligomeric matrix protein as a novel pathogenic factor in keloids. Am J Pathol. 2011 Oct;179(4):1951-60. doi: 10.1016/j.ajpath.2011.06.034. Epub 2011 Aug 26. PMID: 21872564; PMCID: PMC3181360. See more on PubMed
  • Jordan JM, Luta G, Stabler T, Renner JB, Dragomir AD, Vilim V, Hochberg MC,Helmick CG, Kraus VB. Ethnic and sex differences in serum levels of cartilageoligomeric matrix protein: the Johnston County Osteoarthritis Project. Arthritis Rheum. 2003 Mar;48(3):675-81. PubMed PMID: 12632420. See more on PubMed
  • Liang Y, Fu Y, Qi R, Wang M, Yang N, He L, Yu F, Zhang J, Yun CH, Wang X, Liu J, Kong W. Cartilage oligomeric matrix protein is a natural inhibitor of thrombin. Blood. 2015 Aug 13;126(7):905-14. doi: 10.1182/blood-2015-01-621292. Epub 2015 Jun 4. PMID: 26045608. See more on PubMed
  • Liao Q, Kleeff J, Xiao Y, Di Cesare PE, Korc M, Zimmermann A, Büchler MW, Friess H. COMP is selectively up-regulated in degenerating acinar cells in chronic pancreatitis and in chronic-pancreatitis-like lesions in pancreatic cancer. Scand J Gastroenterol. 2003 Feb;38(2):207-15. doi: 10.1080/00365520310000717. PMID: 12678339. See more on PubMed
  • Liu TT, Liu XS, Zhang M, Liu XN, Zhu FX, Zhu FM, Ouyang SW, Li SB, Song CL, Sun HM, Lu S, Zhang Y, Lin J, Tang HM, Peng ZH. Cartilage oligomeric matrix protein is a prognostic factor and biomarker of colon cancer and promotes cell proliferation by activating the Akt pathway. J Cancer Res Clin Oncol. 2018 Jun;144(6):1049-1063. doi: 10.1007/s00432-018-2626-4. Epub 2018 Mar 20. PMID: 29560517. See more on PubMed
  • Magdaleno F, Arriazu E, Ruiz de Galarreta M, Chen Y, Ge X, Conde de la Rosa L, Nieto N. Cartilage oligomeric matrix protein participates in the pathogenesis of liver fibrosis. J Hepatol. 2016 Nov;65(5):963-971. doi: 10.1016/j.jhep.2016.06.003. Epub 2016 Jun 15. PMID: 27318326; PMCID: PMC5831373. See more on PubMed
  • Misumi K, Vilim V, Clegg PD, Thompson CC, Carter SD. Measurement of cartilage oligomeric matrix protein (COMP) in normal and diseased equine synovial fluids.Osteoarthritis Cartilage. 2001 Feb;9(2):119-27. PubMed PMID: 11237659. See more on PubMed
  • Papadakos KS, Bartoschek M, Rodriguez C, Gialeli C, Jin SB, Lendahl U, Pietras K, Blom AM. Cartilage Oligomeric Matrix Protein initiates cancer stem cells through activation of Jagged1-Notch3 signaling. Matrix Biol. 2019 Aug;81:107-121. doi: 10.1016/j.matbio.2018.11.007. Epub 2018 Nov 28. PMID: 30502484. See more on PubMed
  • Papadakos KS, Darlix A, Jacot W, Blom AM. High Levels of Cartilage Oligomeric Matrix Protein in the Serum of Breast Cancer Patients Can Serve as an Independent Prognostic Marker. Front Oncol. 2019 Oct 30;9:1141. doi: 10.3389/fonc.2019.01141. PMID: 31737569; PMCID: PMC6831625. See more on PubMed
  • Skoumal M, Haberhauer G, Feyertag J, Kittl EM, Bauer K, Dunky A. Serum levels of cartilage oligomeric matrix protein are elevated in rheumatoid arthritis, but not in inflammatory rheumatic diseases such as psoriatic arthritis, reactive arthritis, Raynaud's syndrome, scleroderma, systemic lupus erythematosus, vasculitis and Sjögren's syndrome. Arthritis Res Ther. 2004;6(2):73-4. doi: 10.1186/ar1161. Epub 2004 Mar 8. PMID: 15059267; PMCID: PMC400441. See more on PubMed
  • Vilim V, Lenz ME, Vytasek R, Masuda K, Pavelka K, Kuettner KE, Thonar EJ.Characterization of monoclonal antibodies recognizing different fragments ofcartilage oligomeric matrix protein in human body fluids. Arch Biochem Biophys.1997 May 1;341(1):8-16. PubMed PMID: 9143347. See more on PubMed
  • Vilím V, Olejárová M, Machácek S, Gatterová J, Kraus VB, Pavelka K. Serumlevels of cartilage oligomeric matrix protein (COMP) correlate with radiographic progression of knee osteoarthritis. Osteoarthritis Cartilage. 2002Sep;10(9):707-13. PubMed PMID: 12202123. See more on PubMed
  • Vilím V, Vobůrka Z, Vytásek R, Senolt L, Tchetverikov I, Kraus VB, Pavelka K. Monoclonal antibodies to human cartilage oligomeric matrix protein: epitopemapping and characterization of sandwich ELISA. Clin Chim Acta. 2003Feb;328(1-2):59-69. PubMed PMID: 12559599. See more on PubMed
  • Vilím V, Vytásek R, Olejárová M, Machácek S, Gatterová J, Procházka B, KrausVB, Pavelka K. Serum cartilage oligomeric matrix protein reflects the presence ofclinically diagnosed synovitis in patients with knee osteoarthritis.Osteoarthritis Cartilage. 2001 Oct;9(7):612-8. PubMed PMID: 11597173. See more on PubMed
  • Vuga LJ, Milosevic J, Pandit K, Ben-Yehudah A, Chu Y, Richards T, Sciurba J, Myerburg M, Zhang Y, Parwani AV, Gibson KF, Kaminski N. Cartilage oligomeric matrix protein in idiopathic pulmonary fibrosis. PLoS One. 2013 Dec 20;8(12):e83120. doi: 10.1371/journal.pone.0083120. PMID: 24376648; PMCID: PMC3869779. See more on PubMed
  • Zachou K, Gabeta S, Shums Z, Gatselis NK, Koukoulis GK, Norman GL, Dalekos GN. COMP serum levels: A new non-invasive biomarker of liver fibrosis in patients with chronic viral hepatitis. Eur J Intern Med. 2017 Mar;38:83-88. doi: 10.1016/j.ejim.2017.01.007. Epub 2017 Jan 15. PMID: 28100410. See more on PubMed
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