Western blotting, ELISA
Source of Antigen
The antibody was raised in rabbits by immunization with the recombinant Rat Clusterin.
Amino Acid Sequence
The immunization antigen (26.5 kDa) is a protein containing 240 AA of recombinant Rat Clusterin. N-Terminal T7-Tag and C-Terminal His-tag, 25 extra AA (highlighted).
The amino acid sequence of the recombinant Rat Clusterin is 100% homologous to the amino acid sequence (AA 146–360) of the Rat Clusterin precursor
Immunoaffinity chromatography on a column with immobilized recombinant Rat Clusterin.
0.1 mg (determined by BCA method, BSA was used as a standard)
The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.
We advise you to centrifuge this product vial before use. Add 0.1 ml of deionized water, mix gently and let the lyophilized pellet dissolve completely (at least 15 minutes with occasionally gently shaking, not to foam). Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
At ambient temperature. Upon receipt, store the product at the temperature recommended below.
The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.
Quality Control Test
Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody
This product is for research use only.