| Cat. No. | Size |
Price |
|
|---|---|---|---|
| RA19023R | 96 wells (1 kit) |
Competitive ELISA
Urine
On ice. Upon receipt, store the product at the temperature recommended below.
Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
7,8–1000 pg/mL
10 pg/mL
CV 2,7-12,9%
CV 13,2-17,9%
CV 3,3-15,7%
This DHN-MA ELISA Kit is a competitive assay that can be used for quantification of DHN-MA in urine samples. The ELISA typically displays an IC50 (50% B/B0) of approximately 75 pg/ml and a detection limit (80% B/B0) of approximately 10 pg/ml. Measurement of 4-hydroxy-2-nonenal (HNE), an aldehyde formed during the lipid peroxidation process, is one of several accepted biomarkers for lipid peroxidation. 1,4-Dihydroxynonane mercapturic acid (DHN-MA) is the major urinary metabolite of HNE present at physiological levels in rat and human urine. It can be measured without extraction and offers an alternative lipid peroxidation biomarker aside from 8-isoprostane or TBARS.
Oxidative stress
Lipid peroxidation is a well-established mechanism of cellular injury in both plants and animals and is used as an indicator of oxidative stress in cells and tissues. Measurement of 4-hydroxy-2-nonenal (HNE), an aldehyde formed during the lipid peroxidation process, is one of several accepted biomarkers for lipid peroxidation. 1,4-Dihydroxynonane mercapturic acid (DHN-MA) is the major urinary metabolite of HNE present at physiological levels in rat and human urine. It can be measured without extraction and offers an alternative lipid peroxidation biomarker aside from 8-isoprostane or TBARS.