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Fastin™ Elastin Assay (Standard Kit Size)

  • Regulatory status:RUO
  • Type:Colorimetric assay
  • Species:Multispecies
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RBCF2000 110 assays (1 kit)
PubMed Product Details
Technical Data

Type

Colorimetric assay

Applications

Tissue extract, Cell culture and/or animal studies, Cell

Sample Requirements

A sample volume of between 50 and 500 μl is required, containing not less than 5 μg and not more than 70 μg elastin.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store the kit at RT or 2–8°C. Under these conditions, the kit is stable until the expiration date (see label on the box).

Calibration Curve

Calibration Range

5 – 70 μg

Limit of Detection

5 μg

Summary

Features

  • The Fastin™ Elastin Assay is a quantitative dye-binding method for the analysis of elastins released into tissue culture medium and extracted from biological materials.
  • Elastin forms that can be measured by the Fastin Assay as α-elastin: - - soluble tropoelastins; - - lathyrogenic elastins; - - insoluble elastins (following solubilization to elastin polypeptides [α-elastin, κ-elastin])
  • The Fastin Elastin Assay is a quantitative dye-binding method for the analysis of elastins extracted from mammalian sources.
  • The dye label employed is 5,10,15,20-tetraphenyl-21H,23H-porphine tetra-sulfonate (TPPS).
  • Assay run time - 4 hours

Research topic

Extracellular matrix

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