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Fetuin-B Human (HEK), Sheep Polyclonal Antibody

  • Regulatory status:RUO
  • Type:Polyclonal Antibody
  • Other names:16G2, Fetuin-like protein IRL685, Gugu, FETUB
  • Species:Human
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Cat. No. Size Price


RD184172100 0.1 mg
PubMed Product Details
Technical Data

Type

Polyclonal Antibody

Applications

Western blotting, ELISA

Source of Antigen

HEK293

Hosts

Sheep

Preparation

The antibody was raised in sheep by immunization with the recombinant Human Fetuin-B.

Amino Acid Sequence

MW: 41.8 kDa (calculated), migrates at ~ 55 kDa on SDS-PAGE; N-terminal linker (4 extra AA highlighted), C-terminal His-tag (6 extra AA highlighted).

HSHVCGAMSPPQLALNPSALLSRGCNDSDVLAVAGFALRDINKDRKDGYVLRLNRVNDAQEYRRGGLGSLFYLTLDVLETDCHVLRKKAWQDCGMRIFFESVYGQCKAIFYMNNPSRVLYLAAYNCTLRPVSKKKIYMTCPDCPSSIPTDSSNHQVLEAATESLAKYNNENTSKQYSLFKVTRASSQWVVGPSYFVEYLIKESPCTKSQASSCSLQSSDSVPVGLCKGSLTRTHWEKFVSVTCDFFESQAPATGSENSAVNQKPTNLPKVEESQQKNTPPTDSPSKAGPRGSVQYLPDLDDKNSQEKGPQEAFPVHLDLTTNPQGETLDISFLFLEPMEEKLVVLPFPKEKARTAECPGPAQNASPLVLPPHHHHHH

Species Reactivity

Human. Not yet tested in other species.

Purification Method

Immunoaffinity chromatography on a column with immobilized recombinant Human Fetuin-B.

Antibody Content

0.1 mg (determined by BCA method, BSA was used as a standard)

Formulation

The antibody is lyophilized in 0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE.

Reconstitution

Add 0.2 ml of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.

Shipping

At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

The lyophilized antibody remains stable and fully active until the expiry date when stored at –20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles and store frozen at –80°C. Reconstituted antibody can be stored at 4°C for a limited period of time; it does not show decline in activity after one week at 4°C.

Quality Control Test

Indirect ELISA – to determine titer of the antibody SDS PAGE – to determine purity of the antibody

Note

This product is for research use only.

Summary

Research topic

Bone and cartilage metabolism, Energy metabolism and body weight regulation, Immune Response, Infection and Inflammation, Oncology, Others

Summary

Fetuin B is a member of cystatin superfamily proteins, comprises two cystatin domains. It was found in secreted form in serum, it is expressed in liver, tongue and placenta. It regulates osteogenesis by inhibition effect on mineralization. It plays a role in oocyte maturation.

References to Summary

References to Fetuin-B

  • Arnaud P, Miribel L, Emerson DL. Alpha 2-HS glycoprotein. Methods Enzymol. 1988;163:431-41
  • Choi JW, Aseer KR, Chaudhari HN, Mukherjee R, Choi M, Yun JW. Gender dimorphism in regulation of plasma proteins in streptozotocin-induced diabetic rats. Proteomics. 2013 Aug;13 (16):2482-94
  • Choi JW, Liu H, Mukherjee R, Yun JW. Downregulation of fetuin-B and zinc-alpha2-glycoprotein is linked to impaired fatty acid metabolism in liver cells. Cell Physiol Biochem. 2012;30 (2):295-306
  • Denecke B, Graber S, Schafer C, Heiss A, Woltje M, Jahnen-Dechent W. Tissue distribution and activity testing suggest a similar but not identical function of fetuin-B and fetuin-A. Biochem J. 2003 Nov 15;376 (Pt 1):135-45
  • Dietzel E, Wessling J, Floehr J, Schafer C, Ensslen S, Denecke B, Rosing B, Neulen J, Veitinger T, Spehr M, Tropartz T, Tolba R, Renne T, Egert A, Schorle H, Gottenbusch Y, Hildebrand A, Yiallouros I, Stocker W, Weiskirchen R, Jahnen-Dechent W. Fetuin-B, a liver-derived plasma protein is essential for fertilization. Dev Cell. 2013 Apr 15;25 (1):106-12
  • Gharbi M, Sanchez C, Mazzucchelli G, De Pauw E, Henrotin Y. Identification of differential pattern of protein expression in canine osteoarthritis serum after anterior cruciate ligament transection: a proteomic analysis. Vet J. 2013 Sep;197 (3):848-53
  • Hsu SJ, Nagase H, Balmain A. Identification of Fetuin-B as a member of a cystatin-like gene family on mouse chromosome 16 with tumor suppressor activity. Genome. 2004 Oct;47 (5):931-46
  • Murakami T, Walczak R, Caron S, Duhem C, Vidal V, Darteil R, Staels B. The farnesoid X receptor induces fetuin-B gene expression in human hepatocytes. Biochem J. 2007 Nov 1;407 (3):461-9
  • Olivier E, Soury E, Risler JL, Smih F, Schneider K, Lochner K, Jouzeau JY, Fey GH, Salier JP. A novel set of hepatic mRNAs preferentially expressed during an acute inflammation in rat represents mostly intracellular proteins. Genomics. 1999 May 1;57 (3):352-64
  • Olivier E, Soury E, Ruminy P, Husson A, Parmentier F, Daveau M, Salier JP. Fetuin-B, a second member of the fetuin family in mammals. Biochem J. 2000 Sep 1;350 Pt 2:589-97
  • Palviainen M, Raekallio M, Vainionpaa M, Kosonen S, Vainio O. Proteomic profiling of dog urine after European adder (Vipera berus berus) envenomation by two-dimensional difference gel electrophoresis. Toxicon. 2012 Dec 1;60 (7):1228-34
  • Pan TL, Wang PW, Al-Suwayeh SA, Huang YJ, Fang JY. Toxicological effects of cationic nanobubbles on the liver and kidneys: biomarkers for predicting the risk. Food Chem Toxicol. 2012 Nov;50 (11):3892-901
  • Rosenling T, Stoop MP, Attali A, van Aken H, Suidgeest E, Christin C, Stingl C, Suits F, Horvatovich P, Hintzen RQ, Tuinstra T, Bischoff R, Luider TM. Profiling and identification of cerebrospinal fluid proteins in a rat EAE model of multiple sclerosis. J Proteome Res. 2012 Apr 6;11 (4):2048-60
  • Schroeder AC, Schultz RM, Kopf GS, Taylor FR, Becker RB, Eppig JJ. Fetuin inhibits zona pellucida hardening and conversion of ZP2 to ZP2f during spontaneous mouse oocyte maturation in vitro in the absence of serum. Biol Reprod. 1990 Nov;43 (5):891-7
  • Woltje M, Tschoke B, von Bulow V, Westenfeld R, Denecke B, Graber S, Jahnen-Dechent W. CCAAT enhancer binding protein beta and hepatocyte nuclear factor 3beta are necessary and sufficient to mediate dexamethasone-induced up-regulation of alpha2HS-glycoprotein/fetuin-A gene expression. J Mol Endocrinol. 2006 Apr;36 (2):261-77
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