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Manufactured by BioVendor

Ghrelin Acylated Human Express ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, AChE-labelled antibody
  • Other names:M46, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, MTLRP, UNQ524/PRO1066
  • Species:Human
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Cat. No. Size Price

RA194062400R 96 wells (1 kit)
PubMed Product Details
Technical Data


Sandwich ELISA, AChE-labelled antibody



Sample Requirements

20 µl/well


On ice. Upon receipt, store the product at the temperature recommended below.


Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Range

2–250 pg/ml

Limit of Detection

The Limit of Determination, calculated as the concentration of acylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.3 pg/ml and 0.8 pg/ml for long and short first incubation period, respectively for long and short immunological reaction.

Intra-assay (Within-Run)

CV = 8.0% (20h at +4°C)
CV = 7.0% (3h at RT)

Inter-assay (Run-to-Run)

CV = 8.4% (20h at +4°C)
CV = 8.2% (3h at RT)

Spiking Recovery

101.6% (20h at +4°C)
111.0% (3h at RT)

Dilution Linearity




  • The kit measures plasma acylated ghrelin.
  • Special blood collection procedure is needed.
  • Quality controls are human-plasma based.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation


Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.

Product References (7)


  • Yu AP, Ugwu FN, Tam BT, Lee PH, Lai CW, Wong CSC, Lam WW, Sheridan S, Siu PM. One Year of Yoga Training Alters Ghrelin Axis in Centrally Obese Adults With Metabolic Syndrome. Front Physiol. 2018 Sep 20;9:1321. doi: 10.3389/fphys.2018.01321. eCollection 2018. PubMed PMID: 30294284. PubMed CentralPMCID: PMC6158302. See more on PubMed
  • Yu AP, Ugwu FN, Tam BT, Lee PH, Lai CW, Wong CSC, Siu PM. Ghrelin Axis Reveals the Interacting Influence of Central Obesity and Hypertension. Front Endocrinol (Lausanne). 2018 Sep 12;9:534. doi: 10.3389/fendo.2018.00534. eCollection 2018. PubMed PMID: 30258404. PubMed CentralPMCID: PMC6145011. See more on PubMed
  • Leclerc L, Thorin C, Flanagan J, Biourge V, Serisier S, Nguyen P. Higher neonatal growth rate and body condition score at 7 months are predictive factors of obesity in adult female Beagle dogs. BMC Vet Res. 2017 Apr 13;13(1):104. doi: 10.1186/s12917-017-0994-7. PubMed PMID: 28407742. PubMed CentralPMCID: PMC5390368. See more on PubMed
  • Kaliora AC, Kanellos PT, Gioxari A, Karathanos VT. Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. J Med Food. 2017 Mar;20(3):301-308. doi: 10.1089/jmf.2016.0123. Epub 2017 Feb 7. PubMed PMID: 28170279. See more on PubMed
  • Tvarijonaviciute A, Martínez-Subiela S, Ceron JJ. Validation of two ELISA assays for total ghrelin measurement in dogs. J Anim Physiol Anim Nutr (Berl). 2012 Feb;96(1):1-8. doi: 10.1111/j.1439-0396.2010.01112.x. Epub 2010 Dec 28. PubMed PMID: 21198962. See more on PubMed
  • Moshtaghi-Kashanian GR, Razavi F. Ghrelin and leptin levels in relation to puberty and reproductive function in patients with beta-thalassemia. Hormones (Athens). 2009 Jul-Sep;8(3):207-13. doi: 10.14310/horm.2002.1237. PubMed PMID: 19671520. See more on PubMed
  • Meier U, Gressner AM. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem. 2004 Sep;50(9):1511-25. doi: 10.1373/clinchem.2004.032482. Epub 2004 Jul 20. PubMed PMID: 15265818. See more on PubMed
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