Type
Sandwich ELISA, AChE-labelled antibody
Applications
Plasma-EDTA+PHMB
Sample Requirements
20 µl/well
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
2–250 pg/ml
Limit of Detection
The Limit of Determination, calculated as the concentration of acylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.3 pg/ml and 0.8 pg/ml for long and short first incubation period, respectively for long and short immunological reaction.
Intra-assay (Within-Run)
CV = 8.0% (20h at +4°C)
CV = 7.0% (3h at RT)
Inter-assay (Run-to-Run)
CV = 8.4% (20h at +4°C)
CV = 8.2% (3h at RT)
Spiking Recovery
101.6% (20h at +4°C)
111.0% (3h at RT)
Dilution Linearity
89,20%