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Ghrelin Acylated Human Express ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, AChE-labelled antibody
  • Other names:M46, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, MTLRP, UNQ524/PRO1066
  • Species:Human
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Cat. No. Size Price


RA194062400R 96 wells (1 kit)
PubMed Product Details
Technical Data

Type

Sandwich ELISA, AChE-labelled antibody

Applications

Plasma-EDTA+PHMB

Sample Requirements

20 µl/well

Storage/Expiration

Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Curve

Calibration Range

1.96–250 pg/ml

Limit of Detection

The Limit of Determination, calculated as the concentration of acylated ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.3 pg/ml and 0.8 pg/ml for long and short first incubation period, respectively for long and short immunological reaction.

Intra-assay (Within-Run)

CV = 8.0% (20h at +4°C)
CV = 7.0% (3h at RT)

Inter-assay (Run-to-Run)

CV = 8.4% (20h at +4°C)
CV = 8.2% (3h at RT)

Spiking Recovery

101.6% (20h at +4°C)
111.0% (3h at RT)

Dilutation Linearity

89,20%

Summary

Features

  • The kit measures plasma acylated ghrelin.
  • Special blood collection procedure is needed.
  • Quality controls are human-plasma based.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation

Summary

Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.

References to Product

References

  • Kaliora AC, Kanellos PT, Gioxari A, Karathanos VT. Regulation of GIP and Ghrelin in Healthy Subjects Fed on Sun-Dried Raisins: A Pilot Study with a Crossover Trial Design. J Med Food. 2017 Mar;20(3):301-308. doi: 10.1089/jmf.2016.0123
  • Leclerc L, Thorin C, Flanagan J, Biourge V, Serisier S, Nguyen P. Higher neonatal growth rate and body condition score at 7 months are predictive factors of obesity in adult female Beagle dogs. BMC Vet Res. 2017 Apr 13;13(1):104. doi: 10.1186/s12917-017-0994-7
  • Meier U, Gressner AM. Endocrine regulation of energy metabolism: review of pathobiochemical and clinical chemical aspects of leptin, ghrelin, adiponectin, and resistin. Clin Chem. 2004 Sep;50 (9):1511-25
  • Moshtaghi-Kashanian GR, Razavi F. Ghrelin and leptin levels in relation to puberty and reproductive function in patients with beta-thalassemia. Hormones (Athens). 2009 Jul-Sep;8 (3):207-13
  • Tvarijonaviciute A, Martinez-Subiela S, Ceron JJ. Validation of two ELISA assays for total ghrelin measurement in dogs. J Anim Physiol Anim Nutr (Berl. 2010 Dec 28;
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