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Ghrelin Acylated Mouse/Rat Express ELISA

  • Regulatory status:RUO
  • Type:Sandwich ELISA, AChE-labelled antibody
  • Other names:M46, Appetite-regulating hormone, Growth hormone secretagogue, Growth hormone-releasing peptide, Motilin-related peptide, Protein M46, GHRL, MTLRP, UNQ524/PRO1066
  • Species:Mouse, Rat
Cat. No. Size Price

RA394062400R 96 wells (1 kit) $906
PubMed Product Details
Technical Data


Sandwich ELISA, AChE-labelled antibody



Sample Requirements

20 µl/well


On ice. Upon receipt, store the product at the temperature recommended below.


Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).

Calibration Range

250 - 2 pg/ml

Limit of Detection

The limit of detection, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.8 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is less than 5 pg/mL.

Intra-assay (Within-Run)

CV = 6,8% (20h at +4°C) CV = 5.7% (3h at RT)

Inter-assay (Run-to-Run)

CV = 7 % (20h at +4°C) CV = 6.1% (3h at RT)

Spiking Recovery

99.5% (20h at +4°C) 98.6% (3h at RT)

Dilution Linearity

105 %



  • The kit measures plasma acylated ghrelin.
  • Special blood collection procedure is needed.
  • Quality controls are rat-plasma based.

Research topic

Diabetology - Other Relevant Products, Energy metabolism and body weight regulation, Animal studies


Ghrelin, an endogenous ligand for the growth hormone secretagogue receptor, is synthesized principally in the stomach. It stimulates food intake and transduces signals to hypothalamic regulatory nuclei that controls energy homeostasis. The peptide consists of 28 amino acids, with a n-octanoylation of the serine-3 residue, which is necessary for the biological activity mentioned below. Ghrelin is present in the peripheral circulation under two forms: acylated and unacylated.

Product References (1)


  • van Ginhoven TM, Huisman TM, van den Berg JW, Ijzermans JN, Delhanty PJ, de Bruin RW. Preoperative fasting induced protection against renal ischemia/reperfusion injury is independent of ghrelin in mice. Nutr Res. 2010 Dec;30(12):865-9. doi: 10.1016/j.nutres.2010.09.014. PubMed PMID: 21147370. See more on PubMed
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