Type
Sandwich ELISA, AChE-labelled antibody
Applications
Plasma-EDTA+PHMB
Sample Requirements
20 µl/well
Shipping
On ice. Upon receipt, store the product at the temperature recommended below.
Storage/Expiration
Store the kit at -20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Calibration Range
250 - 2 pg/ml
Limit of Detection
The limit of detection, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.8 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is less than 5 pg/mL.
Intra-assay (Within-Run)
CV = 6,8% (20h at +4°C) CV = 5.7% (3h at RT)
Inter-assay (Run-to-Run)
CV = 7 % (20h at +4°C) CV = 6.1% (3h at RT)
Spiking Recovery
99.5% (20h at +4°C) 98.6% (3h at RT)
Dilution Linearity
105 %