Sandwich ELISA, AChE-labelled antibody
Store the kit at –20°C. Under these conditions, the kit is stable until expiration date (see label on the box).
Limit of Detection
The limit of detection, calculated as the concentration of Acylated Ghrelin corresponding to the NSB average (n = 8) plus three standard deviations is 0.8 pg/mL. Due to the minimal plasma dilution (1:5), the limit of detection in the samples is less than 5 pg/mL.
CV = 8.2% (20h at +4°C) CV = 5.5% (3h at RT)
CV = 8.2% (20h at +4°C) CV = 6.5% (3h at RT)
90.0% (20h at +4°C) 99.8% (3h at RT)