Two-Tailed RT/ PCR Primers
Frozen. Upon receipt, store the product at the temperature recommended below.
Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).
Below, links to independent papers developing and using Two-Tailed PCR can be found
- Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
- Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
- Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
- Click here for more papers citing Two-tailed PCR
- For research use only.
- For measurements specific miRNA in biological fluids.
- Components of the kit are provided ready to use.
Cardiovascular disease, Immune Response, Infection and Inflammation, Oncology, Neurodegenerative disease
MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.
Let-7b has been found to act as a tumor suppressor, in order to halt cell proliferation, adhesion, and invasion by targeting the genes of PKA1, DIAPH2, RDX, Ras, c-myc, and HMGA2 proteins. Let-7b expression is differently regulated in breast cancer and the data reveal its possible role in DNA repair capacity during breast carcinogenesis. Similarly, miR-let-7b was shown to be significantly downregulated in osteosarcoma tissues and cell lines and the functional studies revealed that the antitumor effect of miR-let-7b was probably due to targeting and suppressing IGF1R expression.
Let-7b has differential expression patterns also in inflamed tissues compared with healthy controls. The data demonstrate that overexpression of miR-let-7b caused a marked decrease in the expression of the proinflammatory genes, whereas blocking of miR-let-7b caused a reciprocal increase in cytokine expression. Circulating miR-let-7b-5p together with miR-125b-5p function as regulators of the inflammatory response in endometriosis. It was published that up-regulation of miR-let-7b was characteristic of prostatic tumor-associated macrophages and might play a vital role in regulating macrophage polarization, thus modulating the prognosis of prostate cancer. Furthermore, results suggest that miR-let-7b contributes to the epithelial immune responses against H. pylori infection. It has also been shown that miR-let-7b may protect human mesenchymal stem cells implanted into infarcted myocardium from apoptosis and autophagy.
Moreover, miR-let-7b has been linked to neurodegeneration; elevated amounts of miR-let-7b were found in the cerebrospinal fluid of patients with Alzheimer’s disease. In addition, miR-let-7b might be a promising blood-derived miRNA biomarker in multiple sclerosis.