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Manufactured by BioVendor

hsa-miR-142-5p Two-Tailed PRIMERs

  • Regulatory status:RUO
  • Type:Two-Tailed RT/ PCR Primers
  • Species:Human
Cat. No. Size Price

RDTT0000433PRI 50 rxn/ 150 rxn
PubMed Product Details
Technical Data

Don't forget

For successful completion of the assay, we strongly recommend purchasing of our Two-Tailed cDNA Synthesis System and Two-Tailed qPCR Master Mix. The combination of these three products will provide you with the highest quality results.


Two-Tailed RT/ PCR Primers


Frozen. Upon receipt, store the product at the temperature recommended below.


Store the kit at -20°C. Under these conditions, assay components are stable till the expiry date is over. (See the expiry date indicated on the kit label).


Below, links to independent papers developing and using Two-Tailed PCR can be found

  • Androvic P, Valihrach L, Elling J, Sjoback R, Kubista M. Two-tailed RT-qPCR: a novel method for highly accurate miRNA quantification. Nucleic Acids Res. 2017 Sep 6;45(15):e144. doi: 10.1093/nar/gkx588. PMID: 28911110; PMCID: PMC5587787. See more on PubMed
  • Damayanti F, Lombardo F, Masuda JI, Shinozaki Y, Ichino T, Hoshikawa K, Okabe Y, Wang N, Fukuda N, Ariizumi T, Ezura H. Functional Disruption of the Tomato Putative Ortholog of HAWAIIAN SKIRT Results in Facultative Parthenocarpy, Reduced Fertility and Leaf Morphological Defects. Front Plant Sci. 2019 Oct 14;10:1234. doi: 10.3389/fpls.2019.01234. PMID: 31681360; PMCID: PMC6801985. See more on PubMed
  • Anna BB, Grzegorz B, Marek K, Piotr G, Marcin F. Exposure to High-Intensity Light Systemically Induces Micro-Transcriptomic Changes in Arabidopsis thaliana Roots. Int J Mol Sci. 2019 Oct 16;20(20):5131. doi: 10.3390/ijms20205131. PMID: 31623174; PMCID: PMC6829545. See more on PubMed
  • Click here for more papers citing Two-tailed PCR


  • For research use only.
  • For measurements specific miRNA in biological fluids.
  • Components of the kit are provided ready to use.

Research topic

Cardiovascular disease, Oncology, Renal disease


MicroRNAs (miRNAs) are small non-coding RNA molecules, approximately 22 nucleotides in length that regulate gene translation through silencing or degradation of target mRNAs. They are involved in multiple biological processes, including differentiation and proliferation, metabolism, hemostasis, apoptosis or inflammation, and in the pathophysiology of many diseases. Numerous studies have suggested circulating miRNAs as promising diagnostic and prognostic biomarkers of many diseases.

The gene encoding hsa-miR-142-5p is located on the human chromosome 17. hsa-miR-142-5p is highly specific for hematopoietic cells and is overexpressed in pathological conditions such as cancer, immunologically related disorders, small bowel inflammation, renal fibrosis where inflammation occurs and in biopsies from renal transplant patients with acute rejection. It has been suggested that overexpression of hsa-miR-142-5p in patients with chronic antibody-mediated rejection is associated with immunological disorders rather than renal dysfunction. Furthermore, hsa-miR-142-5p may play an onco-miRNA role in progression of renal cell carcinoma.

hsa-miR-142-5p was shown to be downregulated in hepatocellular carcinoma cells and to suppress hepatocelullar carcinoma cell migration. Moreover hsa-miR-142-5p is related to recurrence risk in gastric cancer patients. miRNAs are also implicated in normal heart development and function as well as in cardiac disorders. Downregulation of hsa-miR-142-5p was described in cardiac hypertrophy. Moreover, altered levels of hsa-miR-142-5p in blood were described in dilated cardiomyopathy.

Summary References (9)

References to miR-142-5p

  • TSANG, Felice Ho-Ching, et al. MicroRNA-142-3p and microRNA-142-5p are downregulated in hepatocellular carcinoma and exhibit synergistic effects on cell motility. Frontiers of medicine, 2015, 9.3: 331-343.
  • ZHANG, X., et al. Combination of hsa-miR-375 and hsa-miR-142-5p as a predictor for recurrence risk in gastric cancer patients following surgical resection. Annals of oncology, 2011, 22.10: 2257-2266.
  • LANDGRAF, Pablo, et al. A mammalian microRNA expression atlas based on small RNA library sequencing. Cell, 2007, 129.7: 1401-1414.
  • DANGER, Richard, et al. Expression of miR-142-5p in peripheral blood mononuclear cells from renal transplant patients with chronic antibody-mediated rejection. PloS one, 2013, 8.4: e60702.
  • ANGLICHEAU, Dany, et al. MicroRNA expression profiles predictive of human renal allograft status. Proceedings of the National Academy of Sciences, 2009, 106.13: 5330-5335.
  • LIU, Lingqi, et al. MicroRNA-142-5p promotes cell growth and migration in renal cell carcinoma by targeting BTG3. American journal of translational research, 2017, 9.5: 2394.
  • SHARMA, Salil, et al. Repression of miR‐142 by p300 and MAPK is required for survival signalling via gp130 during adaptive hypertrophy. EMBO molecular medicine, 2012, 4.7: 617-632.
  • BARSANTI, Cristina, et al. Differential regulation of microRNAs in end-stage failing hearts is associated with left ventricular assist device unloading. BioMed research international, 2015, 2015.
  • VOELLENKLE, Christine, et al. MicroRNA signatures in peripheral blood mononuclear cells of chronic heart failure patients. Physiological genomics, 2010, 42.3: 420-426.
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